벼의 밀양23호/기호벼 재조합자식 유전집단을 이용한 PCR 기반 DNA 마커들로 구성된 분자유전자지도 작성
Development of Rice Molecular Genetic and Physical Map Using PCR-based DNA Markers with the Recombinant Inbred Population Derived from Milyang23/Gihobyeo Cross
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참고문헌
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초록
To construct a molecular genetic map using PCR-based DNA markers with the recombinant inbred population derived from Milyang23/Gihobyeo cross, we analyzed the STS, InDel, RTM, and SSR markers which are mainly able to be analyzed on agarose gel. A genetic map comprised of 224 markers including 37 InDel, 88 STS, 8 RTM and 91 SSR markers was constructed. This map contained 1,425 cM with an average interval size of 6.7 cM. The physical map of these markers was also constructed by analyzing the physical location of each maker using its primer sequences and e-PCR program, and the average physical distance per centimorgan was 250 kbp. Among the mapped markers, 51 (22.8%) showed segregation distortion at 5% significance level. They are mainly located in the middle part of chromosome 3, almost whole chromosome 6, the upper end of chromosome 7,the lower end of chromosome 8, and the upper end of chromosome 12. These molecular genetic and physical maps would provide information on the available markers for mapping genes of useful traits with population derived from the crosses between Japonica varieties and Tongil type or Indica varieties.
Abstract
To construct a molecular genetic map using PCR-based DNA markers with the recombinant inbred population derived from Milyang23/Gihobyeo cross, we analyzed the STS, InDel, RTM, and SSR markers which are mainly able to be analyzed on agarose gel. A genetic map comprised of 224 markers including 37 InDel, 88 STS, 8 RTM and 91 SSR markers was constructed. This map contained 1,425 cM with an average interval size of 6.7 cM. The physical map of these markers was also constructed by analyzing the physical location of each maker using its primer sequences and e-PCR program, and the average physical distance per centimorgan was 250 kbp. Among the mapped markers, 51 (22.8%) showed segregation distortion at 5% significance level. They are mainly located in the middle part of chromosome 3, almost whole chromosome 6, the upper end of chromosome 7,the lower end of chromosome 8, and the upper end of chromosome 12. These molecular genetic and physical maps would provide information on the available markers for mapping genes of useful traits with population derived from the crosses between Japonica varieties and Tongil type or Indica varieties.
참고문헌 (21)
- Causse MA, "Saturated molecular map of the rice genome based on an interspecific backcross population", Genetics, 138, pp.1251~1274 (1994)
- Chen M, "An integrated physical and genetic map of the rice genome", Plant Cell, 14, pp.537~545 (2002)
- 진중현, "Identification of Subspecies-specific STS Markers and Their Association with Segregation Distortion in Rice (Oryza sativa L.)", Journal of Crop Science and Biotechnology, 10(3), pp.175~184 (2007), 한국작물학회
- Cho YG, "Development of RFLP framework map of rice (Oryza sativa L.) using recombinant inbred population derived from Milyang23", Gihobyeo cross, 1998, pp.30, 289~297
- 은무영, "Integrated map of AFLP, SSLP and RFLP markers using a recombinant inbred population of rice (Oryza sativa L.)", THEORETICAL AND APPLIED GENETICS, 97, pp.370~380 (1998), SPRINGER
- Harushima Y, "A high-density rice genetic linkage map with 2275 markers using a single F2 population", Genetics, 148, pp.479~494 (1998)
- IRGSP, "The map-based sequence of the rice genome", Nature, 436, pp.793~800 (2005)
- Kang HJ, "Identification of QTL associated with yield and its components based on molecular map in rice", Korean Journal of Breeding Science, 31, pp.40~47 (1999)
- Kang HJ, "Detection of putative QTL conferring grain size and shape in rice", Korean Journal of Breeding Science, 31, pp.330~335 (1999)
- 곽태순, "벼 밀양 23호 × 기호벼 재조합 자식계통의 지역에 따른 품질 특성 관련 QTL 분석", 한국작물학회지, 49(6), pp.539~545 (2004), 한국작물학회
- 곽태순, "벼 밀양 23호*기호벼 재조합 자식계통의 출수일수 및 미립 형태적 특성의 양적형질 유전자좌(QTL) 분석", 한국국제농업개발학회지, 17(3), pp.192~198 (2005), 한국국제농업개발학회
- Lorieux M, "MapDisto: fast and efficient computation of genetic linkage maps", Molecular Breeding (2012)[DOI]
- McCouch SR, "Molecular mapping of rice chromosomes. Theoretical and Applied Genetics", Theoretical and Applied Genetics, 76, pp.815~829 (1988)
- McCouch SR, "Microsatellite marker development, mapping and applications in rice genetics and breeding", Plant Molecular Biology, 35, pp.89~99 (1997)
- McCouch SR, "Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.)", DNA Research, 9, pp.257~279 (2002)
- Orjuela J, "A universal core genetic map for rice", Theoretical and Applied Genetics, 120, pp.563~572 (2010)
- Panaud O, "Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.)", Molecular and General Genetics, 252, pp.597~607 (1996)
- Rotmistrovsky K, "A web server for performing electronic PCR", Nucleic Acids Research, 32, pp.W108~W112 (2004)
- Shen YJ, "Development of genome-wide DNA polymorphism database for map-based cloning of rice genes", Plant Physiology, 135, pp.1198~1205 (2004)
- Voorrips RE, "MapChart: Software for the graphical presentation of linkage maps and QTLs", The Journal of Heredity, 93, pp.77~78 (2002)
- Xu Y, "Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, doubled haploid, and recombinant inbred populations in rice (Oryza sativa L.)", Molecular and General Genetics, 253, pp.535~545 (1997)