본문 바로가기
KERD
논문KCI 등재

다양한 비생물적 스트레스에 의해 유도되는 RsMYB1 프로모터의 특성분석

Characterization of the RsMYB1 Promoter Induced by Various Abiotic Stresses

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류농학

초록

Plants grown under stress conditions generate excessive reactive oxygen species resulting in cell death. Therefore, plants activatethe protection mechanism via antioxidant accumulation. Anthocyanins are flavonoid-derived secondary metabolites with high antioxidantproperties. In this study, we analyzed and characterized the promoter region of RsMYB1, a positive regulator of anthocyanin biosynthesis. The RsMYB1 promoter was designed with four different fragment lengths (MP1, -1034; MP2, -830; MP3, -633; and MP4, -430 bp), andthen each RsMYB1 promoter region was fused into a GUS gene for Arabidopsis transformation. The expression patterns of the RsMYB1 promoterconstructs were analyzed at different developmental stages and under various abiotic stresses. The GUS expression pattern steadily increasedwith plant growth, and coincided with enzyme activity and a histochemical GUS assay. In response to drought, salt, sucrose, and low temperature,the GUS transcript level was highly expressed in MP4 in parallel with GUS enzyme activity. These assays indicated that the proximal region(-430 to -1 bp) of RsMYB1 was the core sequence that was induced by salt and low temperature. The expression level of RsMYB1 in theleaves of radish was highly activated and was consistent with the anthocyanin content under salt and low temperature conditions. These resultssuggest that induction of the RsMYB1 gene can activate the biosynthesis of anthocyanins, which are expected to help plants adapt to stressconditions due to their antioxidant activity.

Abstract

Plants grown under stress conditions generate excessive reactive oxygen species resulting in cell death. Therefore, plants activatethe protection mechanism via antioxidant accumulation. Anthocyanins are flavonoid-derived secondary metabolites with high antioxidantproperties. In this study, we analyzed and characterized the promoter region of RsMYB1, a positive regulator of anthocyanin biosynthesis. The RsMYB1 promoter was designed with four different fragment lengths (MP1, -1034; MP2, -830; MP3, -633; and MP4, -430 bp), andthen each RsMYB1 promoter region was fused into a GUS gene for Arabidopsis transformation. The expression patterns of the RsMYB1 promoterconstructs were analyzed at different developmental stages and under various abiotic stresses. The GUS expression pattern steadily increasedwith plant growth, and coincided with enzyme activity and a histochemical GUS assay. In response to drought, salt, sucrose, and low temperature,the GUS transcript level was highly expressed in MP4 in parallel with GUS enzyme activity. These assays indicated that the proximal region(-430 to -1 bp) of RsMYB1 was the core sequence that was induced by salt and low temperature. The expression level of RsMYB1 in theleaves of radish was highly activated and was consistent with the anthocyanin content under salt and low temperature conditions. These resultssuggest that induction of the RsMYB1 gene can activate the biosynthesis of anthocyanins, which are expected to help plants adapt to stressconditions due to their antioxidant activity.

참고문헌 (39)

  1. Ahmed NU, "Anthocyanin biosynthesis for cold and freezing stress tolerance and desirable color in Brassica rapa", Funct Integr Genomics, 15, pp.383~394 (2015)
  2. An JP, "The ERF transcription factor MdERF38 promotes drought stress-induced anthocyanin biosynthesis in apple", Plant J, 101, pp.573~589 (2020)
  3. Apel K, "Reactive oxygen species : metabolism, oxidative stress, and signal transduction", Annu Rev Plant Biol, 55, pp.373~399 (2004)
  4. Baxter A, "ROS as key players in plant stress signalling", J Exp Bot, 65, pp.1229~1240 (2014)
  5. Bleecker AB, "Last exit : senescence, abscission, and meristem arrest in Arabidopsis", Plant Cell, 9, pp.1169~1179 (1997)
  6. Cirillo V, "Anthocyanins are key regulators of drought stress tolerance in tobacco", Biology, 10, pp.39~ (2021)
  7. Clough SJ, "Floral dip : a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana", Plant J, 16, pp.735~743 (1998)
  8. Corbesier L, "The role of carbohydrates in the induction of flowering in Arabidopsis thaliana : comparison between the wild type and a starchless mutant", Planta, 206, pp.131~137 (1998)
  9. Cramer GR, "Supplemental manganese improves the relative growth, net assimilation and photosynthetic rates of salt-stressed barley", Physiol Plant, 84, pp.600~605 (1992)
  10. Dietz KJ, "Redox-and reactive oxygen species-dependent signaling into and out of the photosynthesizing chloroplast", Plant Physiol, 171, pp.1541~1550 (2016)
  11. Eryılmaz F, "The relationships between salt stress and anthocyanin content in higher plants", Biotechnol Biotechnol Equip, 20, pp.47~52 (2006)
  12. Gechev TS, "Reactive oxygen species as signals that modulate plant stress responses and programmed cell death", Bioessays, 28, pp.1091~1101 (2006)
  13. Golldack D, "Tolerance to drought and salt stress in plants : unraveling the signaling networks", Front Plant Sci, 5, pp.151~ (2014)
  14. Gupta AK, "Sugar signalling and gene expression in relation to carbohydrate metabolism under abiotic stresses in plants", J Biosci, 30, pp.761~776 (2005)
  15. Huang H, "Mechanisms of ROS regulation of plant development and stress responses", Front Plant Sci, 10, pp.800~ (2019)
  16. Huang S, "The roles of mitochondrial reactive oxygen species in cellular signaling and stress response in plants", Plant Physiol, 171, pp.1551~1559 (2016)
  17. Dursun Kisa, "Responses of the phenolic compounds of Zea mays under heavy metal stress", Applied Biological Chemistry, 59(6), pp.813~820 (2016), 한국응용생명화학회
  18. Kovinich N, "Abiotic stresses induce different localizations of anthocyanins in Arabidopsis", Plant Signal Behav, 10, pp.e1027850~ (2015)
  19. Lim SH, "Alternative splicing of the basic helix-loop-helix transcription factor gene CmbHLH2 affects anthocyanin biosynthesis in ray florets of chrysanthemum (Chrysanthemum morifolium)", Front Plant Sci, 12, pp.669315~ (2021)
  20. Lim SH, "A radish basic helix-loop-helix transcription factor, RsTT8 acts a positive regulator for anthocyanin biosynthesis", Front Plant Sci, 8, pp.1917~ (2017)
  21. Lim SH, "Activation of anthocyanin biosynthesis by expression of the radish R2R3-MYB transcription factor gene RsMYB1", Plant Cell Rep, 35, pp.641~653 (2016)
  22. Ljung K, "New mechanistic links between sugar and hormone signalling networks", Current Opinion in Plant Biology, 25, pp.130~137 (2015)
  23. Mittler R, "ROS are good", Trends Plant Sci, 22, pp.11~19 (2017)
  24. Mizoi J, "AP2", ERF family transcription factors in plant abiotic stress responses, 2012, pp.1819, 86~96
  25. Mouradov A, "Flavonoids : a metabolic network mediating plants adaptation to their real estate", Front Plant Sci, 5, pp.620~ (2014)
  26. Naing AH, "Overexpression of snapdragon Delila(Del)gene in tobacco enhances anthocyanin accumulation and abiotic stress tolerance", BMC Plant Biol, 17, pp.1~14 (2017)
  27. Pourcel L, "Flavonoid oxidation in plants : from biochemical properties to physiological functions", Trends Plant Sci, 12, pp.29~36 (2007)
  28. Rolland F, "Sugar sensing and signaling in plants", Plant Cell, 14, pp.S185~S205 (2002)
  29. Rook F, "Genetic approaches to understanding sugar-response pathways", J Exp Bot, 54, pp.495~501 (2003)
  30. Sandalio LM, "Peroxisomes sense and respond to environmental cues by regulating ROS and RNS signalling networks", Ann Bot, 116, pp.475~485 (2015)
  31. Sanghera GS, "Engineering cold stress tolerance in crop plants", Current Genomics, 12, pp.30~ (2011)
  32. Smeekens S, "Sugar-induced signal transduction in plants", Annu Rev Plant Physiol Plant Mol Biol, 51, pp.49~81 (2000)
  33. Solfanelli C, "Sucrose-specific induction of the anthocyanin biosynthetic pathway in Arabidopsis", Plant Physiol, 140, pp.637~646 (2006)
  34. Sun W, "Carbon-nitrogen interaction modulates plant growth and expression of metabolic genes in rice", J Plant Growth Regul, 32, pp.575~584 (2013)
  35. Teng S, "Sucrose-specific induction of anthocyanin biosynthesis in arabidopsis requires the MYB75", PAP1 gene, 2005, pp.139, 1840~1852
  36. Weiss D, "Regulation of flower pigmentation and growth : multiple signaling pathways control anthocyanin synthesis in expanding petals", Physiol Plant, 110, pp.152~157 (2000)
  37. Xin Z, "Cold comfort farm : the acclimation of plants to freezing temperatures", Plant Cell Environ, 23, pp.893~902 (2001)
  38. Xu W, "Transcriptional control of flavonoid biosynthesis by MYB-bHLH-WDR complexes", Trends Plant Sci, 20, pp.176~185 (2015)
  39. Xu Z, "ROS induces anthocyanin production via late biosynthetic genes and anthocyanin deficiency confers the hypersensitivity to ROS-generating stresses in Arabidopsis", Plant Cell Physiol, 58, pp.1364~1377 (2017)

관련 논문AI추천

이 간행물의 다른 논문 (22건)