저항성 유전자 도입과 벼 후대계통의 열악형질간의 관계분석
Effect of Resistance Genes on the Occurrence of Rice Undesirable Characters in a Wide Cross
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Interspecific and intersubspecific crosses have been an important strategy for rice breeding programs to obtain useful charactersby expanding genetic diversity. However, the wide cross routinely causes undesirable characters in rice, such as spikelet sterility and poorquality. In this study, we developed near-isogenic lines of R-gene (Xa4+xa5+Xa21+Pi40+Bph18) introgression population to investigate thegenetic relationship between the occurrence of poor characters and R-genes induced by MAS. The ANOVA analysis revealed that Pi40, Bph18,and Xa4 were associated with spikelet fertility (SF), ratio of ripened grain (RRG), third internode length (TIL), panicle extraction (PE), andprotein content (Pro), whereas others showed no association with the 14 traits tested. In the three-way ANOVA, the impact of two R-genes(Pi40 and Bph18) on the poor characters was independent, with no interaction with others. Quantitative trait locus (QTL) analysis identifiedQTLs for PE and TIL traits near Pi40, and in particular, QTLs for five traits (SF, RRG, TIL, PE, and Pro) were negatively affected aroundBph18. The occurrence of a poor character in R-gene pyramiding lines could be associated with linkage drag caused by the Bph18 gene. We speculate that the development of DNA markers that effectively eliminate poor characters will not only improve breeding efficiency usingMAS, but will also help improve traits of japonica rice through wide crossing.
Abstract
Interspecific and intersubspecific crosses have been an important strategy for rice breeding programs to obtain useful charactersby expanding genetic diversity. However, the wide cross routinely causes undesirable characters in rice, such as spikelet sterility and poorquality. In this study, we developed near-isogenic lines of R-gene (Xa4+xa5+Xa21+Pi40+Bph18) introgression population to investigate thegenetic relationship between the occurrence of poor characters and R-genes induced by MAS. The ANOVA analysis revealed that Pi40, Bph18,and Xa4 were associated with spikelet fertility (SF), ratio of ripened grain (RRG), third internode length (TIL), panicle extraction (PE), andprotein content (Pro), whereas others showed no association with the 14 traits tested. In the three-way ANOVA, the impact of two R-genes(Pi40 and Bph18) on the poor characters was independent, with no interaction with others. Quantitative trait locus (QTL) analysis identifiedQTLs for PE and TIL traits near Pi40, and in particular, QTLs for five traits (SF, RRG, TIL, PE, and Pro) were negatively affected aroundBph18. The occurrence of a poor character in R-gene pyramiding lines could be associated with linkage drag caused by the Bph18 gene. We speculate that the development of DNA markers that effectively eliminate poor characters will not only improve breeding efficiency usingMAS, but will also help improve traits of japonica rice through wide crossing.
참고문헌 (25)
- 최임수, "중생 복합내병성 고품질 벼 ‘청운’", 한국육종학회지, 47(2), pp.166~172 (2015), 한국육종학회[DOI]
- Jena KK, "High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18 (t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L.)", Theor Appl Genet, 112, pp.288~297 (2006)
- Jena KK, "Development of 25 near-isogenic lines (NILs) with ten BPH resistance genes in rice (Oryza sativa L.): production, resistance spectrum, and molecular analysis", Theor Appl Genet, 130, pp.2345~2360 (2017)
- Jeung JU, "A novel gene, Pi40(t), linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice", Theor Appl Genet, 115, pp.1163~1177 (2007)
- 정지웅, "고온조건에서 등숙이 양호하고 도열병에 강한 조생 고품질 벼 ‘중모1024’ 육성", 한국육종학회지, 48(1), pp.72~84 (2016), 한국육종학회[DOI]
- Kashiwagi T, "Identification and characteristics of quantitative trait locus for grain protein content, TGP12, in rice (Oryza sativa L.)", Euphytica, 214, pp.165~ (2018)
- 김우재, "분자표지를 이용한 Bph18 보유 벼멸구 저항성 자포니카 계통 육성", 한국국제농업개발학회지, 25(4), pp.378~384 (2013), 한국국제농업개발학회[DOI]
- Kubo T, "Epistasis underlying female sterility detected in hybrid breakdown in a Japonica-Indica cross of rice (Oryza sativa L.)", Theor Appl Genet, 110, pp.346~355 (2005)
- 곽도연, "망실을 이용한 벼 바이러스병 저항성 대량 검정체계", 한국작물학회지, 52(2), pp.129~133 (2007), 한국작물학회
- 손재근, "벼멸구 저항성 여교잡 집단의 등숙특성 QTL 분석", 한국육종학회지, 37(5), pp.295~299 (2005), 한국육종학회
- 이종희, "DNA 마커를 이용한 벼멸구 저항성 선발 및 복합내병충성 벼 계통 육성", 한국육종학회지, 43(5), pp.413~421 (2011), 한국육종학회
- Liu S, "Genome-wide association studies of ionomic and agronomic traits in USDA mini core collection of rice and comparative analyses of different mapping methods", BMC Plant Biol, 20, pp.441~ (2020)
- McCouch SR, "Gene nomenclature system for rice", Rice, 1, pp.72~84 (2008)
- Meng L, "QTL IciMapping : integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations", Crop J, 3, pp.269~283 (2015)
- 박현수, "벼흰잎마름병 균계 K1 및 K3a 접종에 따른 조생종 벼 품종의 수량성 및 품질변이", 한국육종학회지, 44(4), pp.516~525 (2012), 한국육종학회[DOI]
- 박현수, "약배양 이용 벼멸구, 흰잎마름병 및 줄무늬잎마름병 저항성 복합 내병충성 벼 계통 육성", 한국육종학회지, 46(1), pp.78~89 (2014), 한국육종학회[DOI]
- 박현수, "표현형 및 분자표지 선발 이용 벼흰잎마름병 저항성 유전자 집적자포니카 벼 계통 개발", 한국육종학회지, 48(2), pp.140~158 (2016), 한국육종학회[DOI]
- RDA, "Standard of analysis and survey for agricultural research", ~ (2012)
- Ruengphayak S, "Pseudo-backcrossing design for rapidly pyramiding multiple traits into a preferential rice variety", Rice, 8, pp.7~ (2015)
- 서명철, "기후변화에 따른 국내 벼 품종과 재배기술의 적응성에 관한 고찰", 한국작물학회지, 65(4), pp.327~338 (2020), 한국작물학회
- Suh JP, "Development of elite breeding lines conferring Bph18 gene-derived resistance to brown planthopper (BPH) by marker-assisted selection and genome-wide background analysis in japonica rice (Oryza sativa L.)", Field Crops Res, 120, pp.215~222 (2011)
- 서정필, "분자육종법을 이용한 벼멸구 저항성 고품질 자포니카 벼 신품종 ‘안미’ 육성", 한국육종학회지, 46(2), pp.152~159 (2014), 한국육종학회[DOI]
- 서정필, "Development of Resistant Gene-Pyramided Japonica Rice for Multiple Biotic Stresses Using Molecular Marker-Assisted Selection", Plant Breeding and Biotechnology, 3(4), pp.333~345 (2015), 한국육종학회[DOI]
- Yara A, "Development of near-isogenic lines for BPH25 (t)and BPH26 (t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stål.) in indica rice ‘ADR52’", Breed Sci, 60, pp.639~647 (2010)
- Zhao DD, "Identific ation of candidate gene for internode length in rice to enhance resistance to lodging using qtl analysis", Plants, 10, pp.1369~ (2021)