돌콩(Glycine soja)의 침수 스트레스 관련 유전자 발굴을 위한 대량전사체 분석
Transcriptome Analysis for Flooding Stress-related Gene Identification in Glycine soja
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Sybeans have been grown by plant breeding for decades. As soybeans have very limited genetic variation, it is difficult for soybeanbreeders to find new genetic resources for abiotic stressors. Recently, soybeans have been exposed to flooding stress from intensive summerrainfall owing to climate change. Glycine soja, a wild soybean, is known to have greater genetic variation and greater resistance to a varietyof biotic and abiotic stresses than ordinary soybeans. In this study, high-throughput transcriptome analysis was performed using flood-treatedGlycine soja. Differentially expressed genes (DEGs) were analyzed using reads mapped to reference sequences, and gene ontology (GO) andKyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed using selected DEGs. In addition, RT-qPCR analysis was performedto further analyze the expression of specific candidate genes. Several novel genes that could explain various mechanisms related to water stresswere identified as related transcripts and adaptation mechanisms through cell wall expansion, alcoholic fermentation under anaerobic conditions,and structural changes. In addition, most of the isoflavonoid daidzein pathway genes exhibited upregulated expression under flooding stress. Interestingly, expression of the DIR (dirigent protein 1-like) gene, which is known to decrease in response to flooding stress in soybeans (Glycinemax), was upregulated in Glycine soja. The expression of DIR revealed that DIR may play a key role in conferring flooding stress resistancein Glycine soja. This study provides useful information regarding the genes and comprehensive adaptation mechanisms related to flooding stresstolerance that can be utilized for cultivated soybeans through the Korean wild soybean.
Abstract
Sybeans have been grown by plant breeding for decades. As soybeans have very limited genetic variation, it is difficult for soybeanbreeders to find new genetic resources for abiotic stressors. Recently, soybeans have been exposed to flooding stress from intensive summerrainfall owing to climate change. Glycine soja, a wild soybean, is known to have greater genetic variation and greater resistance to a varietyof biotic and abiotic stresses than ordinary soybeans. In this study, high-throughput transcriptome analysis was performed using flood-treatedGlycine soja. Differentially expressed genes (DEGs) were analyzed using reads mapped to reference sequences, and gene ontology (GO) andKyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed using selected DEGs. In addition, RT-qPCR analysis was performedto further analyze the expression of specific candidate genes. Several novel genes that could explain various mechanisms related to water stresswere identified as related transcripts and adaptation mechanisms through cell wall expansion, alcoholic fermentation under anaerobic conditions,and structural changes. In addition, most of the isoflavonoid daidzein pathway genes exhibited upregulated expression under flooding stress. Interestingly, expression of the DIR (dirigent protein 1-like) gene, which is known to decrease in response to flooding stress in soybeans (Glycinemax), was upregulated in Glycine soja. The expression of DIR revealed that DIR may play a key role in conferring flooding stress resistancein Glycine soja. This study provides useful information regarding the genes and comprehensive adaptation mechanisms related to flooding stresstolerance that can be utilized for cultivated soybeans through the Korean wild soybean.
참고문헌 (94)
- Alam I, "Proteome analysis soybean roots under waterlogging stress at an early vegetative stage", J Biosci, 35, pp.49~62 (2010)
- Aleem M, "Comprehensive RNA-seq analysis revealed molecular pathways and genes associated with drought tolerance in wild soybean(Glycine soja Sieb. and Zucc. )", Physiol Plant, 172, pp.707~732 (2020)
- Arasan SKT, "Characterization and expression analysis of dirigent family genes related to stresses in Brassica", Plant Physiol Biochem, 67, pp.144~153 (2013)
- Ashburner M, "Gene Ontology : tool for the unification of biology", Nat Genet, 25, pp.25~29 (2000)
- Banerjee S, "GST profile expression study in som selected plants : in silico approach", Mol Cell Biochem, 380, pp.283~300 (2009)
- Bohnert HJ, "Plant stress adaptationsmaking metabolism move", Curr Opin Plant Biol, 1, pp.267~274 (1998)
- Chaudhary J, "Expanding omics resources for improvement of soybean seed composition traits", Front Plant Sci, 6, pp.1021~ (2015)
- Chaves MM, "How plants cope with water stress in the field? Photosynthesis and growth", Ann Bot, 89, pp.907~916 (2002)
- Chen W, "Identification and comparative analysis of differential gene expression in soybean leaf tissue under drought and flooding stress revealed by RNA-seq", Front Plant Sci, 7, pp.1044~ (2016)
- Chen Y, "A comprehensive expression analysis of the expansin gene family in potato(Solanum tuberosum)discloses stressresponsive expansin-like B genes for drought and heat tolerances", Plos One, 14, pp.e0219837~ (2019)
- Clouse SD, "BRASSINOSTEROIDS : Essential regulators of plant growth and development", Annu Rev Plant Physiol Plant Mol Biol, 49, pp.427~451 (1998)
- Concibido VC, "Introgression of a quantitative trait locus for yield from Glycine soja into commercial soybean cultivars", Theor Appl Genet, 106, pp.575~582 (2003)
- Cowan IR, "Physiological plant ecology II", pp.589~614 (1982), Springer
- Crawford RMM, "Alcohol dehydrogenase activity in relation to flooding tolerance in roots", J Exp Bot, 18, pp.458~464 (1967)
- Dai F, "RhNAC2 and RhEXPA4 are involved in the regulation of dehydration tolerance during the expansion of rose petals", Plant Physiol, 160, pp.2064~2082 (2012)
- Dahuja A, "Role of ATP-binding cassette transporters in maintaining plant homeostasis under abiotic and biotic stresses", Physiol Plant (2020)
- Danquah A, "The role of ABA and MAPK signaling pathways in plant abiotic stress responses", Biotechnol Adv, 32, pp.40~52 (2014)
- Dastmalchi M, "Transcriptomic evidence for the control of soybean root isoflavonoid content by regulation of overlapping phenylpropanoid pathways", BMC Genom, 18, pp.70~ (2017)
- Dat JF, "Sensing and signaling during plant flooding", Plant Physiol Biochem, 42, pp.273~282 (2004)
- Dhaubhadel S, "Isoflavonoid biosynthesis and accumulation in developing soybean seeds", Plant Mol Biol, 53, pp.733~743 (2003)
- Do THT, "Functions of ABC transporters in plant growth and development", Curr Opin Plant Biol, 41, pp.32~38 (2018)
- Drew MC, "Oxygen deficiency and root metabolism : injury and acclimation under hypoxia and anoxia", Annu Rev Plant Physiol Plant Mol Biol, 48, pp.223~250 (1997)
- Drew MC, "Programmed cell death and aerenchyma formation in roots", Trends Plant Sci, 5, pp.123~127 (2000)
- Feng X, "GsSnRK1 interplays with transcription factor GsERF7, from wild soybean to regulate soybean stress resistance", Plant Cell Environ, 43, pp.1192~1211 (2020)
- Fujiwara K, "Differential expression and elution behavior of basic 7S globulin among cultivars under hot water treatment of soybean seeds", J Biosci Bioeng, 117, pp.742~748 (2014)
- Gamble RL, "Histidine kinase activity of the ETR1 ethylene receptor from Arabidopsis", Proc Natl Acad Sci, 95, pp.7825~7829 (1998)
- Grichko VP, "Ethylene and flooding stress in plants", Plant Physiol Biochem, 39, pp.1~9 (2001)
- Gullner G, "Glutathione S-transferase enzymes in plant-pathogen interactions", Front Plant Sci, 9, pp.1836~ (2018)
- Hamel LP, "Mitogenactivated protein kinase signaling in plant-interacting fungi : distinct messages from conserved messengers", Plant Cell, 24, pp.1327~1351 (2012)
- Han Y, "Characterization of a wheat(Triticum aestivum L. )expansin gene, TaEXPB23, involved in the abiotic stress response and phytohormone regulation", Plant Physiol Biochem, 54, pp.49~58 (2012)
- Hirano H, "Basic 7S globulin in plants", J Proteom, 240, pp.104209~ (2021)
- Ichimura K, "Various abiotic stress rapidly activate Arabidopsis MAP kinase ATMPK4 and ATMPK6", Plant J, 24, pp.655~665 (2000)
- Igamberdiev AU, "The haemoglobin", nitric oxide cycle : involvement in flooding stress and effects on hormone signalling, 2005, pp.96, 557~564
- Kamal AHM, "Gel-free quantitative proteomic approach to identify cotyledon proteins in soybean under flooding stress", J Proteom, 112, pp.1~13 (2015)
- Kamal AHM, "Involvement of reactive oxygen species and mitochondrial proteins in biophoton emission in roots of soybean plants under flooding stress", J Proteome Res, 14, pp.2219~2236 (2015)
- Kim JT, "Changes in the accumulation of isoflavonoids in soybean stress", Agric Res Bull Kyungpook Natl Univ, 12, pp.9~22 (1994)
- Kim SH, "Selection of tolerant and susceptible wild soybean(Glycine soja Siebold & Zucc. )accessions under waterlogging condition using vegetation indices", Pol J Environ Stud, 30, pp.3659~3675 (2021)
- Kim YH, "Comparative analysis of endogenous hormones level in two Soybean(Glycine max L. )lines differing in waterlogging tolerance", Front Plant Sci, 6, pp.714~ (2015)
- 김윤하, "작물의 침수스트레스 저항성 및 회피전략", 농업생명과학연구, 53(2), pp.1~13 (2019), 농업생명과학연구원[DOI]
- Kofsky J, "The untapped genetic reservoir : the past, current, and future applications of the wild soybean(Glycine soja)", Front Plant Sci, 9, pp.949~ (2018)
- Kokubun M, "Genetic and cultural improvement of soybean for waterlogged conditions in Asia", Field Crops Res, 152, pp.3~7 (2013)
- Komatsu S, "Characterization of a novel flooding stress-responsive alcohol dehydrogenase expressed in soybean roots", Plant Mol Biol, 77, pp.309~322 (2011)
- KREI(Korea Rural Economic Institute), "Korea Rural Economic Institute. E04-2021-2", ~ (2021)
- Krishnamurthy P, "Brassica rapa expansin-like B1 gene(BrEXLB1)regulate growth and development in transgenic Arabidopsis and elicits response to abiotic stresses", J Plant Biochem Biotechnol, 28, pp.437~446 (2019)
- Kumar S, "Glutathione S-transferases : role in combating abiotic stresses including arsenic detoxification in plants", Front Plant Sci, 9, pp.751~ (2018)
- Lee JD, "Genetic diversity of wild soybean(Glycine soja Sieb. and Zucc. )accessions from South Korea and other countries", Crop Science, 48, pp.606~ (2008)
- 노경호, "콩에 포함된 비배당체 다이드제인과 제니스테인의 회수", Korean Chemical Engineering Research(HWAHAK KONGHAK), 43(5), pp.641~645 (2005), 한국화학공학회
- Li Q, "Quantitative trait locus(QTLs)mapping for quality traits of wheat based on high density genetic map combined with bulked segregant analysis RNA-seq(BSR-Seq)indicates that the Basic 7S Globulin gene is related to falling number", Front Plant Sci, 11, pp.600788~ (2020)
- Li X, "Transcriptomic analysis of Glycine soja and G. max seedlings and functional characterization of GsGSTU24 and GsGSTU42 genes under submergence stress", Environ Exp Bot, 171, pp.103963~ (2020)
- Li Y, "Expansins and cell growth", Curr Opin Plant Biol, 6, pp.603~610 (2003)
- Li m CW, "Functi on of ABA in stomatal defense against biotic and drought stress", Int J Mol Sci, 16, pp.15251~15270 (2015)
- Lin F, "Breeding for disease resistance in soybean: a global perspective", Theor Appl Genet (2022)[DOI]
- Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method", Methods, 25, pp.402~408 (2001)
- Lü P, "RhEXP4, a rose expansin gene, modulates leaf growth and confers drought and salt tolerance to Arabidopsis", Planta, 237, pp.1547~1559 (2013)
- Mignolet-Spruyt L, "Spreading the news : subcellular and organellar reactive oxygen species production and signalling", J Exp Bot, 67, pp.3831~3844 (2016)
- Muthusamy M, "BrEXLB1, a Brassica rapa Expansin-like B1 gene is associated with root development, drought stress response, and seed germination", Genes, 11, pp.404~ (2020)
- Nanjo Y, "Identification of indicator proteins associated with flooding injury in soybean seedlings using label-free quantitative proteomics", J Proteome Res, 12, pp.4785~4798 (2013)
- Paniagua C, "Dirigent proteins in plants : modulating cell wall metabolism during abiotic and biotic stress", J Exp Bot, 68, pp.3287~3301 (2017)
- 박상용, "대량전사체분석을 통한 국내 수발아 저항성 밀의 유전자 발현 분석", 한국육종학회지, 54(2), pp.104~118 (2022), 한국육종학회
- Qian T, "Arabidopsis synaptotagmin 1 mediates lipid transport in a composition-dependent manner", Traffic, 23, pp.346~356 (2022)
- Ralph SG, "Dirigent proteins in conifer defense II : Extended gene discovery, phylogeny, and constitutive and stress-induced gene expression in spruce(Picea spp. )", Phytochemistry, 68, pp.1975~1991 (2007)
- Rehman SU, "Characterization of vascular plant one-zinc finger(VOZ)in soybean(Glycine max and Glycine soja)and their expression analyses under drought condition", Plos One, 16, pp.e0253836~ (2021)
- Merr. )under flooding stress, "Electron J Plant Breed", 2011, 50~57
- Ruiz-Lopez N, "Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress", Plant Cell, 33, pp.2431~2453 (2021)
- Ruperti B, "Flooding responses on grapevine : A physiological transcriptional, and metabolic perspective", Front Plant Sci, 26, pp.339~ (2019)
- Růžička K, "Ethylene regulates root growth through effects on auxin biosynthesis and transport-dependent auxin distribution", Plant Cell, 19, pp.2197~2212 (2007)
- Sairam RK, "Physiology and biochemistry of waterlogging tolerance in plants", Biol Plant, 52, pp.401~412 (2008)
- Sappl PG, "Proteomic analysis of glutathione S-transferases of Arabidopsis thaliana reveals differential salicylic acid-induced expression of the plant-specific Phi and Tau classes", Plant Mol Biol, 54, pp.205~219 (2004)
- Sasidharan R, "Signal dynamics and interactions during flooding stress", Plant Physiol, 176, pp.1106~1117 (2018)
- Sauter M, "Root responses to flooding", Curr Opin Plant Biol, 16, pp.282~286 (2013)
- Schepetilnikov M, "Auxin signaling in regulation of plant translation reinitiation", Front Plant Sci, 8, pp.1014~ (2017)
- 서창우, "콩 침수 스트레스에 대한 식물생장조절물질 처리 효과", 한국작물학회지, 62(4), pp.325~332 (2017), 한국작물학회[DOI]
- Shabala S, "Membrane transporters mediating root signalling and adptive responses to oxygen deprivation and soil flooding", Plant Cell Environ, 37, pp.2216~2233 (2014)
- Sharmin RA, "RNA-Seq based transcriptomic analysis revealed genes associated with seed-flooding tolerance in wild soybean(Glycine soja Sieb. & Zucc. )", Environ Exp Bot, 171, pp.103906~ (2020)
- Shutov AD, "Soybean basic 7S globulin : subunit heterogeneity and molecular evolution", Biosci Biotechnol Biochem, 74, pp.1631~1634 (2010)
- Steffens B, "Emerging roots alter epidermal cell fate through mechanical and reactive oxygen species signaling", Plant Cell, 24, pp.3296~3306 (2012)
- Strelau M., "Analysis of the Arabidopsis synaptotagmin gene expression and protein accumulation during stress using luciferase-based markers", Doctor, pp.2017
- Teige M, "The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis", Mole Cell, 15, pp.141~152 (2004)
- Theologis A, "Ethylene signaling : redundant receptors all have their say", Curr Biol, 8, pp.R875~R878 (1998)
- Thomas DM, "Functional and biochemical analysis of the C2 domains of Synaptotagmin IV", Mol Biol Cell, 10, pp.2285~2295 (1999)
- Tougou M, "Responses to flooding stress in soybean seedlings with the alcohol dehydrogenase transgene", Plant Biotechnol, 29, pp.301~305 (2012)
- Valliyodan B, "Genetic diversity and genomic strategies for improving drought and waterlogging tolerance in soybeans", J Exp Bot, 68, pp.1835~1849 (2017)
- Vanhee C, "The Arabidopsis multistress regulator TSPO is a heme binding membrane protein and a potential scavenger of porphyrins via an autophagy-dependent degradation mechanism", Plant Cell, 23, pp.782~805 (2011)
- Wang Q, "Photoactivation and inactivation of Arabidopsis cryptochrome 2", Science, 354, pp.343~347 (2016)
- Wang X, "Gel-free", label-free proteomic analysis of root tip of soybean over time under flooding and drought stresses, 2016, pp.130, 42~55
- Warnes GR, "Gplots: Various R programming tools for plotting data", R Package, ~ (2015)
- Wilkinson S, "PH as a stress signal", Plant Growth Regul, 29, pp.87~99 (1999)
- Wu R, "Cloning and expression analysis of a dirigent protein gene from the resurrection plant Boea hygrometrica", Prog Nat Sci, 19, pp.347~352 (2009)
- Xie M, "A referencegrade wild soybean genome", Nat Commun, 10, pp.1216~ (2019)
- Yang Z, "BIC1 acts as a transcriptional coactivator to promote brassinosteroid signaling and plant growth", EMBO J, 40, pp.e104615~ (2021)
- Yin X, "Transcriptomic analysis reveals the flooding tolerant mechanism in flooding tolerant line and abscisic acid treated soybean", Plant Mol Biol, 93, pp.479~496 (2016)
- Zhang J, "Quantitative trait loci underlying flooding tolerance in soybean(Glycine max)", Plant Breed, 141, pp.236~245 (2022)
- Zhao Y, "Control of plant water use by ABA induction of senescence and dormancy : An overlooked lesson from evolution", Plant Cell Physiol, 58, pp.1319~1327 (2017)
- Zhao Y, "Understanding a mechanistic basis of ABA involvement in plant adaptation to soil flooding : the current standing", Plants, 10, pp.1982~ (2021)
