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약물 유도 망막변성 생쥐 모델에서 안구내 면역세포에서의 F4/80와 nestin 발현 양상

Expression Profiles of F4/80 and Nestin in Ocular Immune Cells Following Pharmaceutically Induced Retinal Degeneration in Adult Mice

온영훈|박태관|안상일
대한안과학회 (The Korean Ophthalmological Society)|2013.06|대한안과학회지 (Journal of The Korean Ophthalmological Society)|제54권 제6호|pp. 945-953

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참고문헌

키워드

Retinal degenerationF4/80MNUNestin
주제분류안과학

초록

목적: MNU (N-methyl-N-nitrosourea)로 망막변성을 유도한 생쥐의 섬모체와 시신경에서 F4/80와 nestin의 발현양상을 알아보고자한다. 대상과 방법: 30마리 생쥐를 대상으로 MNU (60 mg/kg)를 복강내 주사한 후 2일, 4일, 7일, 30일에 각각 안구를 적출하여 Hematoxylin and eosin (H&E) 염색 및 Terminal deoxyribonucleotidyl transferase mediated dUTP nick end labeling (TUNEL) 염색, F/80와nestin의 면역조직형광염색을 시행하였다. 결과: MNU 주입후 세포자멸사에 의한 광수용체의 파괴가 나타났다. 면역조직형광염색 결과 대조군에서는 F4/80와 nestin이 함께 발현되지 않았으나, 실험군에서는 시신경 및 섬모체 내부에서 F4/80의 발현이 증가하였고, 이 세포들은 nestin을 함께 발현하였다. 망막의외핵층에서도 F4/80와 nestin을 함께 발현하는 세포가 관찰되었다. 결론: 망막의 손상에 반응하여 F4/80 및 nestin을 함께 발현하는 세포가 섬모체와 시신경을 따라 망막 내부로 이동하여 일시적으로증식 및 활성화된다.

Abstract

Purpose: To evaluate the expression patterns of F4/80 and nestin in the ciliary body and the optic nerve following N-methyl-N-nitrosourea (NMU)-induced retinal degeneration in adult mice. Methods: After intraperitoneal injection of MNU (60 mg/kg) in adult mice, the eyes were enucleated at 2, 4, 7 and 30 days. Hematoxylin and eosin (H&E) stain, terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)stain and immunohistochemical stains of F/80 and nestin were performed. Results: After MNU treatment, the photoreceptors were destroyed by cell apoptosis. According to immunohistochemistry,F4/80 and nestin were not co-expressed in the control group, but F4/80 was expressed within the ciliary body and optic nerve in the MNU-treated group; the expression of nestin also increased. In the outer nuclear layer, F4/80 and nestin co-expressing cells were observed. Conclusions: In response to retinal damage, the F4/80 and nestin co-expressing cells migrated to the retina from the ciliary body and optic nerve and were activated.

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