토끼에서의 빛간섭단층촬영기를 이용한 망막 및 맥락막두께 분석
The Normative Retinal and Choroidal Thicknesses of the Rabbit as Revealed by Spectral Domain Optical Coherence Tomography
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
목적: 토끼에서 스펙트럼영역 빛간섭단층촬영(spectral domain optical coherence tomography, SD-OCT)을 이용하여 시신경유두(optic nerve head, ONH)로부터 거리에 따른 망막 각 층의 두께를 측정하고 이를 통해 망막 동물실험모델에서의 기본 자료를 얻고자 한다. 대상과 방법: 토끼 15마리의 우안에서 SD-OCT 영상을 얻은 후, ONH 테두리에서 시작하여 하방으로 1, 2, 3, 4 그리고 5 mm 위치에서 전체 망막층, 내망막층, 외망막층, 맥락막층, 신경절세포복합체층, 신경절세포층, 내핵층, 그리고 외핵층의 두께를 얻었으며, 측정 위치에 따른 각 층의 두께를 비교하였다. 결과: 총 망막두께(Pearson’s correlation coefficient [CC]=-0.778, p<0.05), 내망막층 두께(CC=-0.710, p<0.05), 외망막층 두께(CC=-0.495, p<0.05), 신경절세포복합체 두께(CC=-0.292, p<0.05), 신경절세포층 두께(CC=-0.284, p<0.05), 그리고 외핵층 두께(CC=-0.760, p<0.05)는 ONH에서 아래로 멀어질수록 감소하였다. 내핵층 두께도 ONH에서의 거리와 음의 상관관계를 보였으나 상관계수는 낮았다(CC=-0.263, p<0.05). 맥락막두께는 ONH에서 멀어질수록 증가하였다(CC=0.511, p<0.05). 결론: SD-OCT를 이용하여 ONH로부터 거리에 따른 토끼 망막두께를 분석하였다. 이 토끼 안저를 이해하는 데 도움이 될 것이며, 토끼 실험의 표준 자료로 사용할 수 있을 것이다.
Abstract
Purpose: We used spectral domain optical coherence tomography (SD-OCT) to assess the retinal and choroidal thicknesses of the rabbit, a commonly used animal model of ophthalmic disease. We report normative datasets. Methods: Semi-automated measurements were made on 15 normal right eyes of New Zealand white rabbits. Total retinal, inner retinal layer, outer retinal layer, choroidal, ganglion cell layer, ganglion cell complex, inner nuclear layer, and outer nuclear layer thicknesses were measured at fixed distances (0, 1, 2, 3, 4, and 5 mm) below the optic nerve head. Results: Total retinal layer (Pearson’s correlation coefficient [CC] = -0.778, p < 0.05), inner retinal layer (CC = -0.710, p < 0.05), outer retinal layer (CC = -0.495, p < 0.05), ganglion cell complex (CC = -0.292, p < 0.05), ganglion cell layer (CC = -0.284, p < 0.05), and outer nuclear layer thicknesses (CC = -0.760, p < 0.05) decreased with the distance from the optic nerve head. Inner nuclear layer thickness correlated negatively with the distance from the optic nerve head, but the correlation coefficient was low (CC = -0.263, p < 0.05). Choroidal thickness increased with the distance from the optic nerve head (CC = 0.511, p < 0.05). Conclusions: Rabbit retinal thicknesses were measured and analyzed by the distance from the optic nerve head. The datasets will serve as standards when using rabbits.
참고문헌 (28)
- 김청환, "스펙트럼 영역 빛간섭단층촬영을 이용한 한국인의 연령과 성별에 따른 황반부 층별 두께 연구", 대한안과학회지, 57(2), pp.264~275 (2016), 대한안과학회
- Gupta P, "Determinants of macular thickness using spectral domain optical coherence tomography in healthy eyes : the Singapore Chinese Eye study", Invest Ophthalmol Vis Sci, 54, pp.7968~7976 (2013)
- 김민환, "특발망막전막에서 유리체절제술 전후 망막층별 두께변화와 시력예후와의 관계", 대한안과학회지, 58(4), pp.420~429 (2017), 대한안과학회
- Ko TH, "Comparison of ultrahighand standard-resolution optical coherence tomography for imaging macular pathology", Ophthalmology, 112, pp.1922.e1~1922.e15 (2005)
- Sayanagi K, "Comparison of retinal thickness measurements between three-dimensional and radial scans on spectral-domain optical coherence tomography", Am J Ophthalmol, 148, pp.431~438 (2009)
- Badaró E, "Spectral-domain optical coherence tomography for macular edema", The Scientific World Journal, 2014, pp.191847~ (2014)
- Ferguson LR, "Retinal thickness measurement obtained with spectral domain optical coherence tomography assisted optical biopsy accurately correlates with ex vivo histology", PLoS One, 9, pp.e111203~ (2014)
- Carpenter CL, "Normative retinal thicknesses in common animal models of eye disease using spectral domain optical coherence tomography", Adv Exp Med Biol, 1074, pp.157~166 (2018)
- Alkin Z, "Quantitative analysis of retinal structures using spectral domain optical coherence tomography in normal rabbits", Curr Eye Res, 38, pp.299~304 (2013)
- Ruggeri M, "In vivo three-dimensional high-resolution imaging of rodent retina with spectral-domain optical coherence tomography", Invest Ophthalmol Vis Sci, 48, pp.1808~1814 (2007)
- Muraoka Y, "Real-time imaging of rabbit retina with retinal degeneration by using spectral-domain optical coherence tomography", PLoS One, 7, pp.e36135~ (2012)
- Penha FM, "Retinal and ocular toxicity in ocular application of drugs and chemicals--part I : animal models and toxicity assays", Ophthalmic Res, 44, pp.82~104 (2010)
- Del Amo EM, "Rabbit as an animal model for intravitreal pharmacokinetics: clinical predictability and quality of the published data", Exp Eye Res, 137, pp.111~124 (2015)
- Chen S, "Animal models of age-related macular degeneration and their translatability into the clinic", Expert Rev Ophthalmol, 9, pp.285~295 (2014)
- Bartuma H, "In vivo imaging of subretinal bleb-induced outer retinal degeneration in the rabbit", Invest Ophthalmol Vis Sci, 56, pp.2423~2430 (2015)
- Lavaud A, "Spectral domain optical coherence tomography in awake rabbits allows identification of the visual streak, a comparison with histology", Transl Vis Sci Technol, 9, pp.13~ (2020)
- Cicinelli MV, "Inner retinal layer and outer retinal layer findings after macular hole surgery assessed by means of optical coherence tomography", J Ophthalmol, 2019, pp.3821479~ (2019)
- Bhagat PR, "Utility of ganglion cell complex analysis in early diagnosis and monitoring of glaucoma using a different spectral domain optical coherence tomography", J Curr Glaucoma Pract, 8, pp.101~106 (2014)
- Anraku A, "Association between optic nerve head microcirculation and macular ganglion cell complex thickness in eyes with untreated normal tension glaucoma and a hemifield defect", J Ophthalmol, 2017, pp.3608396~ (2017)
- Arepalli S, "Assessment of inner and outer retinal layer metrics on the Cirrus HD-OCT Platform in normal eyes", PLoS One, 13, pp.e0203324~ (2018)
- Vaney DI, "The rabbit optic nerve : fibre diameter spectrum, fibre count, and comparison with a retinal ganglion cell count", J Comp Neurol, 170, pp.241~251 (1976)
- Oyster CW, "Density, soma size, and regional distribution of rabbit retinal ganglion cells", J Neurosci, 1, pp.1331~1346 (1981)
- Juliusson B, "Complementary cone fields of the rabbit retina", Invest Ophthalmol Vis Sci, 35, pp.811~818 (1994)
- Hirata M, "Macular choroidal thickness and volume in normal subjects measured by swept-source optical coherence tomography", Invest Ophthalmol Vis Sci, 52, pp.4971~4978 (2011)
- De Schaepdrijver L, "Retinal vascular patterns in domestic animals", Res Vet Sci, 47, pp.34~42 (1989)
- Ninomiya H, "Microvascular architecture of the rabbit eye : a scanning electron microscopic study of vascular corrosion casts", J Vet Med Sci, 70, pp.887~892 (2008)
- 안소민, "Development of a Post-vitrectomy Injection of N-methyl-N-nitrosourea as a Localized Retinal Degeneration Rabbit Model", Experimental Neurobiology, 28(1), pp.62~73 (2019), 한국뇌신경과학회
- Grover S, "Comparison of retinal thickness in normal eyes using stratus and spectralis optical coherence tomography", Invest Ophthalmol Vis Sci, 51, pp.2644~2647 (2010)