토끼에서 고환 사이질조직의 출생 후 성 성숙에 이르는 변화
Changes in the Testis Interstitium of Rabbits from Birth to Sexual Maturity
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토끼에서 출생 후부터 성 성숙에 이르는 시기까지 고환 사이질조직의 변화를 알아보기 위하여 출생후 1, 7, 21, 35, 49, 70, 105, 147, 196 및 252일령(n=8마리/일령)의 토끼를 이용하였다. 토끼의 고환을 2.5% glutaraldehyde를 이용하여 전신 관류고정하고 조직 처리과정을 거쳐 Epon-araldite에 포매하였다. 초박절편기를 사용하여 1 μm로 절편한 다음 methylene blue로 염색하여 고환사이질조직을 이루고 있는 각 구조물의 일반적인 변화상과 형태계측학적인 방법을 적용하여 고환 사이질조직의 변화를 관찰하였다. 1일령의 토끼에서 고환 사이질조직은 고환실질의 66.3%를 나타내었고 성장하면서 점차적으로 감소하여 252일령에 11.8%를 나타내었다. 성숙 사이질세포는 7일령에 처음 관찰되었고 이 시기에 용적치밀도는 0.001%이었으며 성장하면서 증가하여 252일령에는 2.6%에 도달하였다. 사이질세포, 중간엽세포, 혈관내피세포, 혈관주위세포 및 근육모양세포의 절대용적은 출생 후부터 252일령까지 증가하였다(P⁄0.05). 고환조직 당 혈관내피세포,혈관 주위세포, 근육모양세포, 성숙 사이질세포 및 중간엽세포의 총 숫자는 일령에 따라서 점진적으로 증가하였고 출생 후부터 252일령까지 성숙 사이질세포의 생성률이 중간엽세포의 생성률보다 높게 나타났다(P⁄0.05). 중간엽세포의 평균용적은 1일령부터 70일령까지 증가하였으며 70일령에 최고치를 나타내었다. 성숙 사이질세포의 평균용적은 1, 7, 21 및 35일령에는 유의성이 없었으나 이 후 점차적으로 계속 증가하여 196일령과 252일령에는 3배 증가하였다(P⁄0.05). 고환내 총 정자생성과 1일 정자생성은 105일령부터 252일령까지 유의성 있게증가하였다(P⁄0.05). 이러한 결과는 토끼에서 출생 후부터 성 성숙 시기까지 고환 사이질조직을 이루고 있는 각종 세포의 용적치밀도, 절대용적, 평균용적 및 고환내 총 숫자의 변화는 정자발생과 밀접한 연관이 있음을 확인하였다.
Abstract
Changes in the testis interstitium from birth to adulthood were studied in New Zealand white rabbits of 1, 7, 21, 35, 49, 70, 105, 147, 196, and 252 days (n=8 per group) of age. Testes of rabbits were fixed by whole body perfusion using a fixative containing 2.5% glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using 1 μm sections stained with methylene blueazure II, qualitative and quantitative (stereological) morphological studies were performed. Sperm production was measured by routine technique. The volume density of the interstitium represents 66.3% of the testicular parenchyma at day 1. Adult Leydig cells initially detected at day 7 (0.001%), increased progressively to reach a volume density of 2.6% by day 252. The absolute volume of mesenchymal cells, endothelial cells, pericytes, and myoid cells increased from birth to 252 days. The number of endothelial cells, pericytes, and myoid cells per testis increased gradually with age. The absolute numbers of adult Leydig cells and mesenchymal cells per testis increased linearly from birth to 252 days. The rate of production of adult Leydig cells was greater than that of mesenchymal cells in the postnatal rabbit testis through 252 days. The average volume of a mesenchymal cell increased from day 1 to day 70 and declined thereafter; the highest value was at day 70. Total sperm production and daily sperm production per testis increased significantly from 105 to 252 days of age. These results clarified the pattern of changes in the testis interstitium in rabbits from birth to adulthood and correlation these events with spermatogenesis.
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