기도상피에서 eotaxin nitric oxide 생성에 미치는 영향
Effect of Eotaxin on Nitric Oxide Expression in Bronchial Epithelial cells
0
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
Background: Eotaxin has been identified as a key mediator of tissue eosinophil inflammation in asthma or allergic rhinitis. However, the concentration of tissue eotaxin is not elevated in asthmatic patients. Therefore, we hypothesized that some other materials such as proinflammatory cytokines are essential in allergic inflammation affected by eotaxin. Objective: The aim of this study was to establish the influence of eotaxin in vitro on the nitric oxide, which is a marker of pulmonary inflammation in asthma. Method: A549 cells were cultured and stimulated with tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interferon-γ (IFN-γ), and eotaxin in various combined concentration. The concentration of supernatant nitrite was analyzed with Griess reaction method and the expression of inducible nitric oxide synthase (iNOS) was evaluated using a reverse transcriptase polymerase chain reaction (RT-PCR) assay. Result: Only the groups stimulated with both eotaxin and the mixture of TNF-α, IL-1β, and IFN-γ showed the increase of the concentration of supernatant nitrite (P＜0.05) and iNOS expression. In addition, with the mixture of TNF-α, IL-1β, and IFN-γ, the concentration of supernatant nitric oxide was correlated to the concentration of the stimulated eotaxin (correlation coeffient=0.592, P＜0.05). Conclusion: Eotaxin, together with TNF-α, IL-1β, and IFN-γ, could make bronchial epithelial cells (A549) produce nitric oxide and augment the production of nitric oxide.
참고문헌 (22)
- "Eosinophil recruitment to the lung in a murine model of allergic inflammation. The role of T cells, chemokines, and adhesion receptors", 98, pp.2332~2345 (1996)
- "Eotaxin expression in Sephadex-induced lung injury in rats", 155, pp.2001~2008 (1999)
- "CC chemokines and interleukin-5 in bronchial lavage fluid from patients with status asthmaticus. Potential implication in eosinophil recruitment", 162, pp.586~592 (2000)
- "Furchgott RF. The 1989 Ulf von Euler lecture. Studies on endothelium-dependent vasodilation and the endothelium-derived relaxing factor. Acta Physiol Scand 1990", pp.257~70
- "Higgs EA. Biosynthesis of nitric oxide from L-arginine a pathway for the regulation of cell function and communication. Biochem Pharmacol 1989", pp.1709~15
- "Vanhoutte PM. Endothelium and control of vascular function. Hypertension 1989", pp.658~67
- "Ignarro LJ. Biosynthesis and metabolism of endothelium-derived nitric oxide. Annu Rev Pharmacol Toxicol 1990", pp.535~60
- "Human alveolar epithelial cells induced nitric oxide synthase-2 expression in alveolar macrophages.", 19, pp.672~683 (2002)
- "Effects of cysteinyl leukotrienes and leukotriene receptor antagonists on markers of inflammation", 111, pp.S49~S59 (2003)
- "Adaptation of the nitrate reductase and Griess reaction methods for the measurement of serum nitrate plus nitrite levels", 34, pp.193~198 (1997)
- "Expression of matrix metalloproteinases and their endogenous tissue inhibitors in skin lesions from patients with tuberous sclerosis", 51, pp.526~533 (2004)
- "Increased inducible nitric oxide synthase expression and nitric oxide concentration in patients with aplastic anemia", 82, pp.104~108 (2003)
- "Eosinophil chemotactic chemokines (eotaxin, eotaxin-2, RANTES, monocyte chemoattractant protein-3 (MCP- 3), and MCP-4), and C-C chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic (Intrinsic) asthmatics", 163, pp.6321~6329 (1999)
- "Eotaxin-3 but not eotaxin gene expression is upregulated in asthmatics 24 hrs after allergen challenge", 24, pp.682~687 (2001)
- "Kinetics of eotaxin expression and its relationship to eosinophil accumulation and activation in bronchial biopsies and bronchoalveolar lavage (BAL) of asthmatic patients after allergen inhalation", 114, pp.137~146 (1998)
- "Variant eotaxin: its effects on the asthma phenotype", 108, pp.946~953 (2001)
- "Interferon gamma and interleukin 4 stimulate prolonged expression of inducible nitric oxide synthase in human airway epithelium through synthesis of soluble mediators", 100, pp.829~838 (1997)
- "Human lung mononuclear cells induce nitric oxide synthase in murine airway epithelial cells in vitro: role of TNF alpha and IL-1beta", 155, pp.268~273 (1997)
- "Expression and regulation of inducible nitrite oxide synthase from human primary airway epithelial cells", 26, pp.144~151 (2002)
- "Intranasal administration of eotaxin increases nasal eosinophils and nitric oxide in patients with allergic rhinitis", 105, pp.58~64 (2000)
- "Expression of the C-C chemokine receptor CCR3 in human airway epithelial cells", 166, pp.1457~1461 (2001)
- "p38 Mitogen-activated protein kinase-induced glucocorticoid receptor phosphorylation reduces its activity: role in steroid-insensitive asthma", 109, pp.649~657 (2002)