감작된 BALB/c 생쥐에서 Schultz-Dale 반응과 Th2 면역반응에 대한 Dehydroepiandrosterone의 효과
Effects of Dehydroepiandrosterone on the Schultz-Dale Reaction and the Th2 Immune Response in Sensitized BALB/c Mice
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Dehydroepiandrosterone (DHEA) increases the production of interferon (IFN)-γ, and IFN-γ suppresses the Th2 response. Objective: To investigate whether DHEA suppresses the in vitro allergen-induced response of airway muscles. Method: Female BALB/c mice were fed mouse chow containing 0, 0.001, 0.01 or 0.1% DHEA during the study period. The animals were sensitized with ovalbumin (OVA), and then the Schultz-Dale reaction to 50μg/ml OVA, serum OVA-specific IgE concentration and cytokine production by cultured splenocytes were measured. Result: OVA-sensitized mice (n=20) developed a Schultz- Dale reaction, had significantly high concentrations of IgE and IL-5 and a significantly low IFN-γ/IL-5 ratio compared to the controls (n=20). The treatment with 0.1% DHEA significantly decreased the IL-5 level (123 vs. 54 pg/ml, P＜0.001) and tended to increase the IFN-γ/IL-5 ratio (2.2±0.3 vs. 3.7±0.7, P=0.09), but did not suppress the Schultz-Dale reaction and IgE levels. The contractile responses of muscles to OVA were significantly related to OVA-specific IgE levels (r=0.446, P＜0.001) and the IFN-γ/IL-5 ratio (r=−0.427, P＜0.001). Conclusion: DHEA did not suppress the Schultz-Dale reaction or the OVA-specific IgE production despite a significant suppression in the Th2 response in mice. (Korean J Asthma Allergy Clin Immunol 2008;28:121-127)
Abstract
Background: Dehydroepiandrosterone (DHEA) increases the production of interferon (IFN)-γ, and IFN-γ suppresses the Th2 response. Objective: To investigate whether DHEA suppresses the in vitro allergen-induced response of airway muscles. Method: Female BALB/c mice were fed mouse chow containing 0, 0.001, 0.01 or 0.1% DHEA during the study period. The animals were sensitized with ovalbumin (OVA), and then the Schultz-Dale reaction to 50μg/ml OVA, serum OVA-specific IgE concentration and cytokine production by cultured splenocytes were measured. Result: OVA-sensitized mice (n=20) developed a Schultz- Dale reaction, had significantly high concentrations of IgE and IL-5 and a significantly low IFN-γ/IL-5 ratio compared to the controls (n=20). The treatment with 0.1% DHEA significantly decreased the IL-5 level (123 vs. 54 pg/ml, P＜0.001) and tended to increase the IFN-γ/IL-5 ratio (2.2±0.3 vs. 3.7±0.7, P=0.09), but did not suppress the Schultz-Dale reaction and IgE levels. The contractile responses of muscles to OVA were significantly related to OVA-specific IgE levels (r=0.446, P＜0.001) and the IFN-γ/IL-5 ratio (r=−0.427, P＜0.001). Conclusion: DHEA did not suppress the Schultz-Dale reaction or the OVA-specific IgE production despite a significant suppression in the Th2 response in mice. (Korean J Asthma Allergy Clin Immunol 2008;28:121-127)
참고문헌 (27)
- Novak N, "Allergic and nonallergic forms of atopic diseases", J Allergy Clin Immunol, 112, pp.252~262 (2003)
- Stephens NL, "Seow CY: Airway smooth muscle: physiology, bronchomotor tone, pharmacology, and relation to asthma, In Weiss EB, Stein M(eds.): Bronchial asthma mechanisms and therapeutics. 3rd ed", pp.314~332 (1993), Little, Brown & Co., Toronto
- Ricci M, "The importance of Th2-like cells in the pathogenesis of airway allergic inflammation", Clin Exp Allergy, 23, pp.360~369 (1993)
- Choi IS, "Effects of BCG revaccination on asthma", Allergy, 58, pp.1114~1116 (2003)
- Daynes RA, "Regulation of murine lymphokine production in vivo. II. Dehydroepiandrosterone is a natural enhancer of interleukin 2 synthesis by helper T cells", Eur J Immunol, 20, pp.793~802 (1990)
- Araghi-Niknam M, "Eskelson CD, Watson RR. Cytokine dysregulation and increased oxidation is prevented by dehydroepiandrosterone in mice infected with murine leukemia retrovirus", Pro Soc Exp Biol Med, 216, pp.386~391 (1997)
- Feher KG, "Adrenocortical function in bronchial asthma", Acta Medica Hungarica, 40, pp.125~131 (1983)
- Dunn PJ, "Dehydroepiandrosterone sulphate concentrations in asthmatic patients: Pilot study", NZ Med J, 97, pp.805~808 (1984)
- Yu CK, "Attenuation of house dust mite Dermatophagoides farinae-induced airway allergic responses in mice by dehydroepiandrosterone is correlated with down-regulation of Th2 response", Clin Exp Allergy, 29, pp.414~422 (1999)
- Yu CK, "Dehydroepiandrosterone attenuates allergic airway inflammation in Dermatophagoides farinae-sensitized mice", J Microbiol Immunol Infection, 35, pp.199~202 (2002)
- Cui Y, "Suppressive effect of dehydroepiandrosterone on the development of asthmatic reaction in BALB", c mice, 2004, pp.24, 116~126
- Vincenc KS, "Comparison of in vivo and in vitro responses to histamine in human airways", Am Rev Respir Dis, 128, pp.875~879 (1983)
- Roberts JA, "Comparison of in vivo airway responsiveness and in vitro smooth muscle sensitivity to methacholine in man", Thorax, 39, pp.837~843 (1984)
- Sudo N, "The requirement of intestinal bacterial flora for the development of an IgE production system fully susceptible to oral tolerance induction", J Immunol, 159, pp.1739~1745 (1997)
- Cameron L, "Synthesis and regulation of immunoglobulin E, In Adkinson NF Jr, Yunginger JW, Busse WW, Bochner BS, Holgate ST, Simons FER(eds.): Middleton’s allergy principles & practice. 6th ed", pp.87~100 (2003), Mosby, Philadelphia
- Herz U, "The relevance of murine animal models to study the development of allergic bronchial asthma", Immunol Cell Biol, 74, pp.209~217 (1996)
- Mehlhop PD, "Allergen-induced bronchial hyperreactivity and eosinophilic inflammation occur in the absence of IgE in a mouse model of asthma", Proc Natl Acad Sci USA, 94, pp.1344~1349 (1997)
- De Jonste JC, "In vitro responses of airways from an asthmatic patient", Eur J Respir Dis, 71, pp.23~29 (1987)
- Bai TR, "Abnormalities in airway smooth muscle in fatal asthma", Am Rev Respir Dis, 141, pp.552~557 (1990)
- Pennings HJ, "Tumour necrosis factor-alpha induces hyperreactivity in tracheal smooth muscle of the guinea-pig in vitro", Eur Respir J, 12, pp.45~49 (1998)
- Reynolds AM, "Scicchitano R. Cytokines enhance airway smooth muscle contractility in response to acetylcholine and neurokinin A", Respirology, 5, pp.135~160 (2000)
- Koh YI, "BCG infection in allergen-presensitized rats suppresses Th2 immune response and prevents the development of allergic asthmatic reaction", J Clin Immunol, 21, pp.51~59 (2001)
- Black JL, "Sensitization alters contractile responses and calcium influx in human airway smooth muscle", J Allergy Clin Immunol, 84, pp.440~447 (1989)
- Cheng GF, "Regulation of murine interleukin-10 production by dehydroepiandrosterone", J Interferon Cytokine Res, 20, pp.474~478 (2000)
- Groux H, "A CD4+ T-cell subset inhibits antigen-specific T-cell responses and prevents colitis", Nature, 389, pp.737~742 (1997)
- Borish L, "Cytokines in allergic inflammation. In Adkinson NF Jr, Yunginger JW, Busse WW, Bochner BS, Holgate ST, Simons FER(eds.): Middleton’s allergy principles & practice. 6th ed", pp.135~157 (2003), Mosby Inc., Philadelphia
- Hernandez-Pando R, "The effects of androstenediol and dehydroepiandrosterone on the course and cytokine profile of tuberculosis in BALB", c mice, 1998, pp.95, 234~241