Proteomic analysis of differentially expressed skin proteins in iRhom2Uncv mice
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A mouse homozygous for the spontaneous mutation uncovered(Uncv) has a hairless phenotype. A 309-bp non-frameshiftdeletion mutation in the N-terminal cytoplasmic domainof iRhom2 was identified in Uncv mice (iRhom2Uncv) using targetregion sequencing. The detailed molecular basis for howthe iRhom2 mutation causes the hairless phenotype observedin the homozygous iRhom2Uncv mouse remains unknown. Toidentify differentially expressed proteins in the skin of wild-typeand homozygous iRhom2Uncv littermates at postnatal day 5,proteomic approaches, including two-dimensional gel electrophoresisand mass spectrometry were used. Twelve proteinswere differentially expressed in the skin in a comparison betweenwild-type and homozygous iRhom2Uncv mice. A selectionof the proteomic results were tested and verified usingqRT-PCR, western blot and immunohistochemistry. These dataindicate that differentially expressed proteins, especiallyKRT73, MEMO1 and Coro-1, might participate in the mechanismby which iRhom2 regulates the development of murineskin.
Abstract
A mouse homozygous for the spontaneous mutation uncovered(Uncv) has a hairless phenotype. A 309-bp non-frameshiftdeletion mutation in the N-terminal cytoplasmic domainof iRhom2 was identified in Uncv mice (iRhom2Uncv) using targetregion sequencing. The detailed molecular basis for howthe iRhom2 mutation causes the hairless phenotype observedin the homozygous iRhom2Uncv mouse remains unknown. Toidentify differentially expressed proteins in the skin of wild-typeand homozygous iRhom2Uncv littermates at postnatal day 5,proteomic approaches, including two-dimensional gel electrophoresisand mass spectrometry were used. Twelve proteinswere differentially expressed in the skin in a comparison betweenwild-type and homozygous iRhom2Uncv mice. A selectionof the proteomic results were tested and verified usingqRT-PCR, western blot and immunohistochemistry. These dataindicate that differentially expressed proteins, especiallyKRT73, MEMO1 and Coro-1, might participate in the mechanismby which iRhom2 regulates the development of murineskin.
참고문헌 (23)
- Li SR, "Uncv(uncovered) : a new mutation causing hairloss on mouse chromosome 11", Genet Res, 73, pp.233~238 (1999)
- Shi YZ, "A high-resolution genetic and physical map of a mouse coat abnormality locus(Uncv)", Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao, 35, pp.397~402 (2003)
- Fuchs E, "Scratching the surface of skin development", Nature, 445, pp.834~842 (2007)
- Legue E, "Hair follicle renewal:authentic morphogenesis that depends on a complex progression of stem cell lineages", Development, 137, pp.569~577 (2010)
- Alonso L, "The hair cycle", J Cell Sci, 119, pp.391~393 (2006)
- Nagarajan P, "Transcriptional control of the differentiation program of interfollicular epidermal keratinocytes", Crit Rev Eukaryot Gene Expr, 18, pp.57~79 (2008)
- Nakamura M, "Mutant laboratory mice with abnormalities in hair follicle morphogenesis, cycling, and", or structure: an update, 2013, pp.69, 6~29
- Etheridge SL, "Rhomboid proteins: a role in keratinocyte proliferation and cancer", Cell Tissue Res, 351, pp.301~307 (2013)
- Adrain C, "Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE", Science, 335, pp.225~228 (2012)
- McIlwain DR, "iRhom2regulation of TACE controls TNF-mediated protection against Listeria and responses to LPS", Science, 335, pp.229~232 (2012)
- Zettl M, "Rhomboid family pseudoproteases use the ER quality control machinery to regulate intercellular signaling", Cell, 145, pp.79~91 (2011)
- Maretzky T, "iRhom2controls the substrate selectivity of stimulated ADAM17-dependent ectodomain shedding", Proc Natl Acad Sci U S A, 110, pp.11433~11438 (2013)
- Blaydon DC, "RHBDF2mutations are associated with tylosis, a familial esophageal cancer syndrome", Am J Hum Genet, 90, pp.340~346 (2012)
- Porter RM, "Keratin K6irs is specific to the inner root sheath of hair follicles in mice and humans", Br J Dermatol, 145, pp.558~568 (2001)
- Langbein L, "K25(K25irs1), K26(K25irs2), K27(K25irs3), and K28(K25irs4)represent the type I inner root sheath keratins of the human hair follicle", J Invest Dermatol, 126, pp.2377~2386 (2006)
- Botchkarev VA, "Molecular biology of hair morphogenesis: development and cycling", J Exp Zool B Mol Dev Evol, 298, pp.164~180 (2003)
- Langbein L, "Keratins of the human hair follicle", Int Rev Cytol, 243, pp.1~78 (2005)
- Langbein L, "K6irs1, K6irs2, K6irs3, and K6irs4 represent the inner-root-sheath-specific type II epithelial keratins of the human hair follicle", J Invest Dermatol, 120, pp.512~522 (2003)
- Pieters J, "On guard:coronin proteins in innate and adaptive immunity", Nat Rev Immunol, 13, pp.510~518 (2013)
- Marone R, "Memo mediates ErbB2-driven cell motility", Nat Cell Biol, 6, pp.515~522 (2004)
- Sorokin AV, "MEMO1, a new IRS1-interacting protein, induces epithelial-mesenchymal transition in mammary epithelial cells", Oncogene, 32, pp.3130~3138 (2013)
- Haenzi B, "Loss of Memo, a novel FGFR regulator, results in reduced lifespan", FASEB J, 28, pp.327~336 (2013)
- Cui JW, "Two-dimensional electrophoresis protein profiling as an analytical tool for human acute leukemia classification", Electrophoresis, 26, pp.268~279 (2005)