Effects of Murine Dermal Cells on the Regulation of Hair Growth Is Dependent on the Cell Number and Post-Natal Age of Newborn Mice
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Dermal cells from neonatal mice can initiate the formation of hair follicles (HFs) when combined with adult mouse epidermal cells and transplanted subcutaneously into athymic mice. In the present study, the effects of dermal cells on HF formation were tested in terms of total cell number and the time course of cell harvest. Results demonstrated that the number of dermal cells is critical to the formation of HF. Furthermore, hair forming ability is rapidly decreasing as the neonatal mice age. To examine potential differences in gene expression, cDNA array was performed. Results demonstrate that numerous molecules which are directly involved in receptor and signaling correlated with decreased hair inductivity in early time points after delivery. It is reported that bone morphogenic protein (BMP)-6 and Wnt3a treatment increased hair inductivity of dermal papilla cells. But in our study, no changes were observed in the expression levels of BMP-6 and Wnt3a. However, several Wnt related genes demonstrate increased or decreased expression levels. Thus,our results suggest that co-ordinated regulation of these molecules will be important in hair neogenesis within our model system.
Abstract
Dermal cells from neonatal mice can initiate the formation of hair follicles (HFs) when combined with adult mouse epidermal cells and transplanted subcutaneously into athymic mice. In the present study, the effects of dermal cells on HF formation were tested in terms of total cell number and the time course of cell harvest. Results demonstrated that the number of dermal cells is critical to the formation of HF. Furthermore, hair forming ability is rapidly decreasing as the neonatal mice age. To examine potential differences in gene expression, cDNA array was performed. Results demonstrate that numerous molecules which are directly involved in receptor and signaling correlated with decreased hair inductivity in early time points after delivery. It is reported that bone morphogenic protein (BMP)-6 and Wnt3a treatment increased hair inductivity of dermal papilla cells. But in our study, no changes were observed in the expression levels of BMP-6 and Wnt3a. However, several Wnt related genes demonstrate increased or decreased expression levels. Thus,our results suggest that co-ordinated regulation of these molecules will be important in hair neogenesis within our model system.
참고문헌 (10)
- Cotsarelis G, "Epithelial stem cells: a folliculocentric view", J Invest Dermatol, 126, pp.1459~1468 (2006)
- Yang CC, "Review of hair follicle dermal cells", J Dermatol Sci, 57, pp.2~11 (2010)
- Chuong CM, "Defining hair follicles in the age of stem cell bioengineering", J Invest Dermatol, 127, pp.2098~2100 (2007)
- Osada A, "Long-term culture of mouse vibrissal dermal papilla cells and de novo hair follicle induction", Tissue Eng, 13, pp.975~982 (2007)
- Rendl M, "BMP signaling in dermal papilla cells is required for their hair follicle-inductive properties", Genes Dev, 22, pp.543~557 (2008)
- Kishimoto J, "Wnt signaling maintains the hair-inducing activity of the dermal papilla", Genes Dev, 14, pp.1181~1185 (2000)
- Millar SE, "Molecular mechanisms regulating hair follicle development", J Invest Dermatol, 118, pp.216~225 (2002)
- Dai J, "Prostate cancer induces bone metastasis through Wntinduced bone morphogenetic protein-dependent and independent mechanisms", Cancer Res, 68, pp.5785~5794 (2008)
- Xing Y, "Immunolocalization of Wnt5a during the hair cycle and its role in hair shaft growth in mice", Acta Histochem, 113, pp.608~612 (2011)
- Katoh M, "WNT signaling in stem cell biology and regenerative medicine", Curr Drug Targets, 9, pp.565~570 (2008)