Expression of fox-related genes in the skin follicles of Inner Mongolia cashmere goat
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Objective: This study investigated the expression of genes in cashmere goats at different periods of their fetal development. Methods: Bioinformatics analysis was used to evaluate data obtained by transcriptome sequencing of fetus skin samples collected from Inner Mongolia cashmere goats on days 45, 55, and 65 of fetal age. Results: We found that FoxN1, FoxE1, and FoxI3 genes of the Fox gene family were probably involved in the growth and development of the follicle and the formation of hair, which is consistent with previous findings. Real-time quantitative polymerase chain reaction detecting system and Western blot analysis were employed to study the relative differentially expressed genes FoxN1, FoxE1, and FoxI3 in the body skin of cashmere goat fetuses and adult individuals. Conclusion: This study provided new fundamental information for further investigation of the genes related to follicle development and exploration of their roles in hair follicle initiation, growth, and development.
Abstract
Objective: This study investigated the expression of genes in cashmere goats at different periods of their fetal development. Methods: Bioinformatics analysis was used to evaluate data obtained by transcriptome sequencing of fetus skin samples collected from Inner Mongolia cashmere goats on days 45, 55, and 65 of fetal age. Results: We found that FoxN1, FoxE1, and FoxI3 genes of the Fox gene family were probably involved in the growth and development of the follicle and the formation of hair, which is consistent with previous findings. Real-time quantitative polymerase chain reaction detecting system and Western blot analysis were employed to study the relative differentially expressed genes FoxN1, FoxE1, and FoxI3 in the body skin of cashmere goat fetuses and adult individuals. Conclusion: This study provided new fundamental information for further investigation of the genes related to follicle development and exploration of their roles in hair follicle initiation, growth, and development.
참고문헌 (21)
- Paus R, "The biology of hair follicles", N Engl J Med, 341, pp.491~497 (1999)[DOI]
- Hardy MH, "The secret life of the hair follicle", Trends Genet, 8, pp.55~61 (1992)[DOI]
- Maini PK, "The Turing model comes of molecular age", Science, 314(5804), pp.1397~1398 (2007)
- Kaestner KH, "Unified nomenclature for the winged helix", forkhead transcription factors, 2000, pp.14, 142~146
- Palamaro L, "FOXN1 in organ development and human diseases", Int Rev Immunol, 33, pp.83~93 (2014)[DOI]
- Bredenkamp N, "An organized and functional thymus generated from FOXN1-reprogrammed fibroblasts", Nat Cell Biol, 16, pp.902~908 (2014)[DOI]
- Mecklenburg L, "Learning from nudity: lessons from the nude phenotype", Exp Dermatol, 14, pp.797~810 (2005)[DOI]
- Weiner L, "Dedicated epithelial recipient cells determine pigmentation patterns", Cell, 130, pp.932~942 (2007)
- Shirokova V, "Foxi3 deficiency compromises hair follicle stem cell specification and activation", Stem Cells, 34, pp.1896~1908 (2016)[DOI]
- Yanjun Z, "Study on skin follicle morphogenesis,development in the fetal period and hox genes of Inner Mongolian cashmere goats" (2007)
- Jun Y, "Study on the development, growth cycle and related genes of Inner Mongolia cashmere goat" (2004)
- Changqing L, "Hair follicles cycling of cashmere goats and mRNA expression of KAP13-1 during anagen" (2005)
- Alonso L, "The hair cycle", J Cell Sci, 119, pp.391~393 (2006)[DOI]
- Schmidt-Ullrich R, "Molecular principles of hair follicle induction and morphogenesis", BioEssays, 27, pp.247~261 (2005)[DOI]
- Botchkarev VA, "Molecular biology of hair morphogenesis: Development and cycling", J Exp Zool Part B Mol Dev Evol, 298, pp.164~180 (2003)
- Frank J, "Exposing the human nude phenotype", Nature, 398(6727), pp.473~474 (1999)
- Kobielak K, "Loss of a quiescent niche but not follicle stem cells in the absence of bone morphogenetic protein signaling", Proc Natl Acad Sci USA, 104, pp.10063~10068 (2007)[DOI]
- Bamforth JS, "Congenital hypothyroidism, spiky hair, and cleft palate", J Med Genet, 26, pp.49~51 (1989)
- Clifton-Bligh RJ, "Mutation of the gene encoding human TTF-2 associated with thyroid agenesis, cleft palate and choanal atresia", Nat Genet, 19, pp.399~401 (1998)[DOI]
- Prowse DM, "Ectopic expression of the nude gene induces hyperproliferation and defects in differentiation: implications for the self-renewal of cutaneous epithelia", Dev Biol, 212, pp.54~67 (1999)[DOI]
- Lee D, "Association between mouse nude gene expression and the initiation of epithelial terminal differentiation", Dev Biol, 208, pp.362~374 (1999)[DOI]