STK899704 inhibits stemness of cancer stem cells and migration via the FAK-MEK-ERK pathway in HT29 cells
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Colon cancer is one of the most lethal and common malignancies worldwide. STK899704, a novel synthetic agent, has been reported to exhibit anticancer effects towards numerous cancer cells. However, the effect of STK899704 on the biological properties of colon cancer, including cancer cell migration and cancer stem cells (CSCs), remains unknown. Here, we examined the inhibitory effect of STK899704 on cell migration and CSC stemness. In the wound healing assay, STK899704 significantly inhibited the motility of colon cancer cells. Furthermore, STK899704 downregulated the mRNA expression levels of the cell migration mediator focal adhesion kinase (FAK). STK899704 also suppressed mitogen-activated protein kinase kinase and extracellular signal-regulated kinase, which are downstream signaling molecules of FAK. Additionally, STK899704 inhibited stemness gene expression and sphere formation in colon cancer stem cells. These results suggest that STK899704 can be used to treat human colon cancer.
Abstract
Colon cancer is one of the most lethal and common malignancies worldwide. STK899704, a novel synthetic agent, has been reported to exhibit anticancer effects towards numerous cancer cells. However, the effect of STK899704 on the biological properties of colon cancer, including cancer cell migration and cancer stem cells (CSCs), remains unknown. Here, we examined the inhibitory effect of STK899704 on cell migration and CSC stemness. In the wound healing assay, STK899704 significantly inhibited the motility of colon cancer cells. Furthermore, STK899704 downregulated the mRNA expression levels of the cell migration mediator focal adhesion kinase (FAK). STK899704 also suppressed mitogen-activated protein kinase kinase and extracellular signal-regulated kinase, which are downstream signaling molecules of FAK. Additionally, STK899704 inhibited stemness gene expression and sphere formation in colon cancer stem cells. These results suggest that STK899704 can be used to treat human colon cancer.
참고문헌 (32)
- Siegel R, "Cancer statistics for Hispanics", Latinos, 2012, 2012, pp.62, 283~298
- Lozano R, "Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the global burden of disease study 2010", Lancet, 380, pp.2095~2128 (2012)
- Jemal A, "Global cancer statistics", CA Cancer J Clin, 61, pp.69~90 (2011)
- Bak Y, "IL-32theta inhibits stemness and epithelialmesenchymal transition of cancer stem cells via the STAT3 pathway in colon cancer", Oncotarget, 7, pp.7307~7317 (2016)
- Merika E, "Review. Colon cancer vaccines: an update", Vivo, 24, pp.607~628 (2010)
- Bonnet D, "Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell", Nat Med, 3, pp.730~737 (1997)
- Jordan CT, "Cancer stem cells: controversial or just misunderstood?", Cell Stem Cell, 4, pp.203~205 (2009)
- Lessard J, "Bmi-1 determines the proliferative capacity of normal and leukaemic stem cells", Nature, 423, pp.255~260 (2003)
- Lee HE, "An increase in cancer stem cell population after primary systemic therapy is a poor prognostic factor in breast cancer", Br J Cancer, 104, pp.1730~1738 (2011)
- Gao XM, "Properties and feasibility of using cancer stem cells in clinical cancer treatment", Cancer Biol Med, 13, pp.489~495 (2016)
- Vinogradov S, "Cancer stem cells and drug resistance: the potential of nanomedicine", Nanomedicine (Lond), 7, pp.597~615 (2012)
- Jordan MA, "Microtubules as a target for anticancer drugs", Nat Rev Cancer, 4, pp.253~265 (2004)
- Zhou J, "Targeting microtubules for cancer chemotherapy", Curr Med Chem Anticancer Agents, 5, pp.65~71 (2005)
- Sakchaisri K, "Anticancer activity of a novel small molecule tubulin inhibitor STK899704", PLoS One, 12, pp.e0173311~ (2017)
- Gialeli C, "Roles of matrix metalloproteinases in cancer progression and their pharmacological targeting", FEBS J, 278, pp.16~27 (2011)
- Liang W, "Combretastatin A4 regulates proliferation, migration, invasion, and apoptosis of thyroid cancer cells via PI3K", Akt signaling pathway, 2016, pp.22, 4911~4917
- McLean GW, "The role of focal-adhesion kinase in cancer - a new therapeutic opportunity", Nat Rev Cancer, 5, pp.505~515 (2005)
- Pastrana E, "Eyes wide open: a critical review of sphere-formation as an assay for stem cells", Cell Stem Cell, 8, pp.486~498 (2011)
- Al-Hajj M, "Prospective identification of tumorigenic breast cancer cells", Proc Natl Acad Sci U S A, 100, pp.3983~3988 (2003)
- Goldman RD, "The role of three cytoplasmic fibers in BHK-21 cell motility. I. Microtubules and the effects of colchicine", J Cell Biol, 51, pp.752~762 (1971)
- Han YS, "Fucoidan inhibits the migration and proliferation of HT-29 human colon cancer cells via the phosphoinositide-3 kinase", Akt, Mol Med Rep, pp.2015, 12[DOI]
- Mu Y, "FAM83D knockdown regulates proliferation, migration and invasion of colorectal cancer through inhibiting FBXW7", Notch-1signalling pathway, 2017, pp.90, 548~554
- Wang YJ, "MicroRNA-433inhibits oral squamous cell carcinoma cells by targeting FAK", Oncotarget, 8, pp.100227~100241 (2017)
- Deramaudt TB, "Altering FAK-paxillin interactions reduces adhesion, migration and invasion processes", PLoS One, 9, pp.e92059~ (2014)
- Pylayeva Y, "Ras- and PI3K-dependent breast tumorigenesis in mice and humans requires focal adhesion kinase signaling", J Clin Invest, 119, pp.252~266 (2009)
- Schlaepfer DD, "Control of motile and invasive cell phenotypes by focal adhesion kinase", Biochim Biophys Acta, 1692, pp.77~102 (2004)
- Suva ML, "Identification of cancer stem cells in Ewing's sarcoma", Cancer Res, 69, pp.1776~1781 (2009)
- Suetsugu A, "Characterization of CD133+hepatocellular carcinoma cells as cancer stem", progenitor cells, 2006, pp.351, 820~824
- Hurt EM, "CD44+ CD24(-) prostate cells are early cancer progenitor", stem cells that provide a model for patients with poor prognosis, 2008, pp.98, 756~765
- 김원태, "Cancer stem cell surface markers on normal stem cells", BMB Reports, 50(6), pp.285~298 (2017), 생화학분자생물학회
- Bak Y, "A1E inhibits proliferation and induces apoptosis in NCI-H460 lung cancer cells via extrinsic and intrinsic pathways", Mol Biol Rep, 40, pp.4507~4519 (2013)
- Bak Y, "IL-32theta downregulates CCL5 expression through its interaction with PKCdelta and STAT3", Cell Signal, 26, pp.3007~3015 (2014)
