Antimetastatic effect of fucoidan against non-small cell lung cancer by suppressing non-receptor tyrosine kinase and extracellular signal-related kinase pathway
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BACKGROUND/OBJECTIVES: Fucoidan, a polysaccharide content in brown algae, has been reported to inhibit the growth of cancer cells. The present study aimed to investigate the suppression effects of fucoidan on A549 non-small cell lung cancer cells migration. MATERIALS/METHODS: The anti-migrator y activity of fucoidan in A549 cells was examined by wound healing assay and phalloidin-rhodamine staining in response to fucoidan (0–100 µg/mL) treatment for 48 h. Western blot analysis was performed to clarif y the protein expressions relevant to migrator y activity. RESULTS: Fucoidan (25–100 µg/mL) significantly suppressed A549 cells migration together with reduced the intensity of phalloidin-rhodamine which detect filopodia and lamellipodia protrusions at 48 h of treatment. The protein expression indicated that fucoidan significantly suppressed the phosphor ylation of focal adhesion kinase (FAK), Src, and extracellular signal- related kinase (ERK). In addition, the phosphor ylation of p38 in A549 cells was found to be increased. CONCLUSIONS: Our data conclude that fucoidan exhibits anti-migrator y activities against lung cancer A549 cells mediated by inhibiting ERK1/2 and FAK-Src pathway.
Abstract
BACKGROUND/OBJECTIVES: Fucoidan, a polysaccharide content in brown algae, has been reported to inhibit the growth of cancer cells. The present study aimed to investigate the suppression effects of fucoidan on A549 non-small cell lung cancer cells migration. MATERIALS/METHODS: The anti-migrator y activity of fucoidan in A549 cells was examined by wound healing assay and phalloidin-rhodamine staining in response to fucoidan (0–100 µg/mL) treatment for 48 h. Western blot analysis was performed to clarif y the protein expressions relevant to migrator y activity. RESULTS: Fucoidan (25–100 µg/mL) significantly suppressed A549 cells migration together with reduced the intensity of phalloidin-rhodamine which detect filopodia and lamellipodia protrusions at 48 h of treatment. The protein expression indicated that fucoidan significantly suppressed the phosphor ylation of focal adhesion kinase (FAK), Src, and extracellular signal- related kinase (ERK). In addition, the phosphor ylation of p38 in A549 cells was found to be increased. CONCLUSIONS: Our data conclude that fucoidan exhibits anti-migrator y activities against lung cancer A549 cells mediated by inhibiting ERK1/2 and FAK-Src pathway.
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