Neuronal differentiation and developmental characteristics in the dentate gyrus of staggerer mutant mice
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Homozygous staggerer (RORasg/sg) mice showed a severe ataxia caused by cerebellum degeneration. Decreased and dysfunctional Rora is a main cause of this neurologic phenotype. The phenotype of staggerer mice has been well known in cerebellum. However,there has been rarely reported about cerebrum even though of staggerer is expressed in merely cerebellum but hippocampus,thalamus, cortex, and olfactory bulb. The expressions of Ki67,doublecortin (DCX), and NeuN, which are cell proliferation,neuronal differentiation and mature neuron markers, respectively,were measured with immunohistechemistry in dentate gyrus in staggerer mice in order to uncover whether staggerer can affect the change in dentate gyrus. The immunoreactivities of DCX and NeuN were significantly reduced in the dentate gyrus of staggerer mice than normal control, while Ki67 were rarely unchanged in staggerer mice. These results suggest that staggerer mutation has an influence on the neuronal differentiation and development not only in cerebellum but also in dentate gyrus.
Abstract
Homozygous staggerer (RORasg/sg) mice showed a severe ataxia caused by cerebellum degeneration. Decreased and dysfunctional Rora is a main cause of this neurologic phenotype. The phenotype of staggerer mice has been well known in cerebellum. However,there has been rarely reported about cerebrum even though of staggerer is expressed in merely cerebellum but hippocampus,thalamus, cortex, and olfactory bulb. The expressions of Ki67,doublecortin (DCX), and NeuN, which are cell proliferation,neuronal differentiation and mature neuron markers, respectively,were measured with immunohistechemistry in dentate gyrus in staggerer mice in order to uncover whether staggerer can affect the change in dentate gyrus. The immunoreactivities of DCX and NeuN were significantly reduced in the dentate gyrus of staggerer mice than normal control, while Ki67 were rarely unchanged in staggerer mice. These results suggest that staggerer mutation has an influence on the neuronal differentiation and development not only in cerebellum but also in dentate gyrus.
참고문헌 (18)
- Boukhtouche, F., "RORα, a pivotal nuclear receptor for Purkinje neuron survival and differentiation: From development to ageing", Cerebellum, 5, pp.97~104 (2006)
- Frédéric, F., "Enhanced endocrine response to novel environment stress and lack of corticosterone circadian rhythm in staggerer (Rora sg", sg) mutant mice, 2006, pp.83, 1525~1532
- Herrup, K., "Staggerer chimera: intrinsic nature of Purkinje cell defects and implications for normal cerebellar development", Brain Res., 215, pp.49~59 (1979)
- Jarvis, C. I., "Age-related phenotypes in the staggerer mouse expand the RORalpha nuclear re ceptor’s role beyond the cerebellum", Mol. Cell. Endocrinol., 186, pp.1~5 (2002)
- Sidman, R. L., "Staggerer, a new mutation in the mouse affecting the cerebellum", Science, 137, pp.610~612 (1962)
- Sonmez, E., "Ro le of staggerer gene in determining cell number in cerebellar cortex. II. Granule cell death and persistence of the external granule cell layer in young mouse chimeras", Brain Res., 314, pp.271~283 (1984)
- Matsui, T., "An orphan nuclear receptor, mROR alpha, and its spatial expression in adult mouse brain", Mol. Brain Res. Brain Res., 33, pp.217~226 (1995)
- Monnier, Z., "Structural and immunohistological modifications in olfactory bulb of the staggerer mutant mouse", Biol. Cell, 91, pp.29~44 (1999)
- Corcoran, J., "Nerve growth factor acts via retinoic acid synthesis to stimulate neurite outgrowth", Nat. Neurosci., 2, pp.307~308 (1999)
- Misner, D. L., "Vitamin A deprivation results in reversible loss of hippocampal long-term synaptic plasticity", Proc. Natl. Acad. Sci. U.S.A., 98, pp.11714~11719 (2001)
- Wagner, E., "Retinoic acid synthesis in the postnatal mouse brain marks distinct developmental stages and functional systems", Cereb. Cortex, 12, pp.1244~1253 (2002)
- Doulazmi, M., "Cerebellar purkinje cell loss in heterozygous RORA＋", − mice: a longitudinal study, 2006, pp.20, 1~17
- Gold, D. A., "RORα in genetic control of cerebellum development: 50 staggering years", Brain Res., 1140, pp.19~25 (2007)
- Journiac, N., "Quantitative analysis of microglial cells in the degenerating cerebellum of the staggerer (RORAsg", sg) mutant mouse, 2005, pp.19, 143~154
- Hwang, I. K., "Reduced hippocampal cell differentiation in the subgranular zone of the dentate gyrus in a rat model of type II diabetes", Neurochem. Res., 33, pp.394~400 (2008)
- Nacher, J., "Doublecortin expression in the adult rat telecephalon", Eur. J. Neurosci., 14, pp.629~644 (2001)
- Yang, H. K., "Distribution of doublecortin expressing cells near the lateral ventricles in the adult mouse brain", J. Neurosci. Res., 76, pp.282~295 (2004)
- Jacobs, S., "Retinoic acid is required early during adult neurogenesis in the dentate gyrus", Proc. Natl. Acad. Sci. U.S.A., 103, pp.3902~3907 (2006)