Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Paclitaxel is produced by various species of yew trees and has been extensively used to treat tumors. In our research, a taxadiene synthase (TS) gene from Taxus brevifolia was used to transform the roots of cultured ginseng (Panax ginseng C.A. Meyer) to produce taxadiene, the unique skeletal precursor to taxol. The TS gene was successfully introduced into the ginseng genome, and the de novo formation of taxadiene was identified by mass spectroscopy profiling. Without any change in phenotypes or growth difference in a TS-transgenic ginseng line, the transgenic TSS3-2 line accumulated 9.1 μg taxadiene per gram of dry weight. In response to the treatment of methyl jasmonate for 3 or 6 days, the accumulation was 14.6 and 15.9 μg per g of dry weight, respectively. This is the first report of the production of taxadiene by engineering ginseng roots with a taxadiene synthase gene. [BMB Reports 2012; 45(10):589-594]
Abstract
Paclitaxel is produced by various species of yew trees and has been extensively used to treat tumors. In our research, a taxadiene synthase (TS) gene from Taxus brevifolia was used to transform the roots of cultured ginseng (Panax ginseng C.A. Meyer) to produce taxadiene, the unique skeletal precursor to taxol. The TS gene was successfully introduced into the ginseng genome, and the de novo formation of taxadiene was identified by mass spectroscopy profiling. Without any change in phenotypes or growth difference in a TS-transgenic ginseng line, the transgenic TSS3-2 line accumulated 9.1 μg taxadiene per gram of dry weight. In response to the treatment of methyl jasmonate for 3 or 6 days, the accumulation was 14.6 and 15.9 μg per g of dry weight, respectively. This is the first report of the production of taxadiene by engineering ginseng roots with a taxadiene synthase gene. [BMB Reports 2012; 45(10):589-594]
참고문헌 (35)
- Wani, M. C., "Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia", J. Am. Chem. Soc., 93, pp.2325~2327 (1971)
- Kumar, N., "Taxol-induced polymerization of purified tubulin: mechanism of action", J. Biol. Chem., 256, pp.10435~10441 (1981)
- Parness, J., "Taxol binds to polymerized tubulin in vitro", J. Cell Biol., 91, pp.479~487 (1981)
- Jun-Ho Ahn, "Spry2 does not directly modulate Raf-1 kinase activity in v-Ha-ras-transformed NIH 3T3 fibroblasts", BMB Reports, 43(3), pp.205~211 (2010), 생화학분자생물학회
- Dieras, V., "Taxanes in combination with doxorubicin in the treatment of the treatment of metastatic breast cancer", Semin. Oncol., 25, pp.18~22 (1998)
- Sgadari, C., "Mechanism of paclitaxel activity in Kaposi’s sarcoma", J. Immunol., 165, pp.509~517 (2000)
- Pezzutto, J., "Taxol production in plant cell culture comes of age", Nat. Biotechnol., 14, pp.1083~ (1996)
- Rowinsky, E. K., "Paclitaxel(Taxol)", N. Engl. J. Med., 332, pp.1004~1014 (1995)
- Woo, D. D. L., "Taxol inhibits progression of congenital polycystic kidney disease", Nature, 368, pp.750~753 (1994)
- Arsenaulta, A. L., "Taxol involution of collagen- induced arthritis: Ultrastructural correlation with the inhibition of synovitis and neovascularization", Clin. Immunol. Immunopathol., 86, pp.280~289 (1998)
- Vidensek, N., "Taxol content in bark, wood, root, leaf, twig, andseedling from several Taxus species", J. Nat. Prod., 53, pp.1609~1610 (1990)
- Holton, R. A., "First total synthesis of taxol. 2. Completion of the C and D rings", J. Am. Chem. Soc., 116, pp.1599~1600 (1994)
- Holton, R. A., "First total synthesis of taxol. 1. Functionalization of the B ring", J. Am. Chem. Soc., 116, pp.1597~1598 (1994)
- Nicolaou, K. C., "Total synthesis of taxol. 1. Retrosynthesis, degradation, and reconstitution", J. Am. Chem. Soc., 117, pp.624~633 (1995)
- Danishefsky, S. J., "Total synthesis of baccatin III and taxol", J. Am. Chem. Soc., 118, pp.2843~2859 (1996)
- Castor, T. P., "Determination of taxol in Taxus media needles in the presence of interfering components", J. Liqu. Chromatogr., 16, pp.723~731 (1993)
- Stierle, A., "Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of pacific yew", Science, 260, pp.214~216 (1993)
- Li, J. Y., "Endophytic taxol-producing fungi from bald cypress,Taxodium distichum", Microbiol., 142, pp.2223~2226 (1996)
- Kim, J. H., "Production of taxol and related taxanes in Taxus brevifolia cell cultures: effect of sugar", Biotechnol. Lett., 17, pp.101~106 (1995)
- Srinivasan, V., "Taxol production in bioreactors: kinetics of biomass accumulation, nutrient uptake, and taxol production by cell suspension of Taxus baccata", Biotechnol. Bioeng., 47, pp.666~676 (1995)
- Cusidó, R. M., "Improved paclitaxel and baccatin III production in suspension cultures of Taxus media", Biotechnol. Prog., 18, pp.418~423 (2002)
- Zhang, L., "Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures", Proc. Natl. Acad. Sci. U.S.A., 101, pp.6786~6791 (2004)
- David, C., "Conservation of T-DNA in plants regenerated from hairy root cultures", Nature Biotech., 2, pp.73~76 (1984)
- Jung A Kim, "Hairy Root Cultures of Taxus cuspidata for Enhanced Production of Paclitaxel", Journal of the Korean Society for Applied Biological Chemistry, 52(2), pp.144~150 (2009), 한국응용생명화학회
- Walker, K., "Molecules of interest: Taxol biosynthetic genes", Phytochem., 58, pp.1~7 (2001)
- Hezari, M., "Purification and characterization of taxa-4(5),11(12)-diene synthase from pacific yew (Taxus brevifolia) that catalyzes the first committed step of Taxol biosynthesis", Arch. Biochem. Biophys., 322, pp.437~444 (1995)
- Wang, W., "cDNA cloning, expression and characterization of taxadiene synthase, a diterpene cyclase from Taxus chinensis", Acta. Bot. Sin., 44, pp.181~187 (2002)
- Do Yeon Kim, "Ginsenoside Rh2(S) induces the differentiation and mineralization of osteoblastic MC3T3-E1 cells through activation of PKD and p38 MAPK pathways", BMB Reports, 44(10), pp.659~664 (2011), 생화학분자생물학회
- in transgenic tomato fruit, "Transgenic Res.", 2007, 121~126
- Anterola, A., "Production of taxa-4(5),11(12)-diene by transgenicPhyscomitrella patens", Transgenic Res., 18, pp.655~660 (2009)
- Besumbes, O., "Metabolic engineering of isoprenoid biosynthesis in Arabidopsis for the production of taxadiene, the first committed precursor of taxol", Biotechnol. Bioeng., 88, pp.168~175 (2004)
- Yasumura, Y., "Step-by-step acquisition of the gibberellin- DELLA growth-regulatory mechanism during land-plant evolution", Curr. Biol., 17, pp.1225~1230 (2007)
- Hood, E. E., "New Agrobacterium helper plasmids for gene transfer to plants", Transgenic Res., 2, pp.208~218 (1993)
- Valvekens, D., "Agrobacterium tumefaciens-mediated transformationof Arabidopsis thaliana root explants by using kanamycinselection", Proc. Natl. Acad. Sci. U.S.A., 85, pp.5536~5540 (1988)
- Dellaporta, S. L., "A plant DNA minipreparation: Version II", Plant Mol. Biol. Rep., 1, pp.19~21 (1983)