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논문

Factors Affecting Agrobacterium tumefaciens-mediatedTransformation of Panax ginseng C.A. Meyer

김옥태
한국약용작물학회|2007.04|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제15권 제2호|pp. 100-104

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키워드

transformationPanax ginsengAgrobacterium tumefacienshygromycin

초록

A protocol for the production of transgenic Panax ginseng C.A. Meyer was established via Agrobacterium tumfaciens- mediated genetic transformation of direct somatic embryos. A number of conditions related to the co-cultivation were tested with respect to maximizing transformation efficiency. The results showed that pH of the co-cultivation medium (5.7), the bacterial growth phase (optical density; OD600 = 0.8), co-cultivation period (3 days), and acetosyringone concentration (100 μM) had positive effects on transformation. Selected plantlets were cultured on the medium at an elevated hygromycin level (30㎎/ℓ). Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using hpt primers and by Southern hybridization using hpt-specific probe. The transgenic plantlets were obtained after 3-month cultivation and did not show any detectable variation in morphology or growth characteristics compared to wild-type plants.

Abstract

A protocol for the production of transgenic Panax ginseng C.A. Meyer was established via Agrobacterium tumfaciens- mediated genetic transformation of direct somatic embryos. A number of conditions related to the co-cultivation were tested with respect to maximizing transformation efficiency. The results showed that pH of the co-cultivation medium (5.7), the bacterial growth phase (optical density; OD600 = 0.8), co-cultivation period (3 days), and acetosyringone concentration (100 μM) had positive effects on transformation. Selected plantlets were cultured on the medium at an elevated hygromycin level (30㎎/ℓ). Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using hpt primers and by Southern hybridization using hpt-specific probe. The transgenic plantlets were obtained after 3-month cultivation and did not show any detectable variation in morphology or growth characteristics compared to wild-type plants.

참고문헌 (19)

  1. "Temperature-sensitive step in Ti plasmidvir region induction and correlation with cytokinin secretionby Agrobacterium", pp.1~8 (1988)
  2. "Agrobacterium-mediated production of transgenic rice plantsexpressing a chimeric α-amylase promoter", β-glucuronidasegene, 1993, 491~506
  3. "Regenerative ability of somatic single and multiple embryos from cotyledons of Korea ginseng on hormone-free medium", 17, pp.544~551 (1998)
  4. "Rapid and efficient Agrobacterium-mediated transformation of Panax ginseng by plasmolyzing pre-treatment of cotyledons", 20, pp.616~621 (2001)
  5. "Production of herbicide-resistant transgenic Panax ginseng through the introduction of the phosphinothricin acetyl transferase gene and successful soil transfer", 21, pp.563~568 (2003)
  6. "Genetic transformation of Populus deltoids and Populus X euramericana clones using Agrobacterium tumefaciens", 48, pp.53~61 (1997)
  7. "A rapid DNA isolation procedure forsmall quantities of fresh leaf tissue", pp.11~15 (1987)
  8. "The use of the polymerase chain reaction in planttransformation studies", pp.221~224 (1991)
  9. "mediated by Agrobacteriumand sequence analysis of the boundaries of the T-DNA", pp.271~282 (1994)
  10. "Plant transformation technology: Agrobacterium-mediated transformation" (2004), John Wiley & Sons Ltd., England
  11. "Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase gene", 45, pp.976~984 (2004)
  12. "Agrobacterium-mediated transformation of plum", pp.853~857 (1991)
  13. "Increase of root induction in Pinus nigra explants using agrobacteria", 15, pp.610~614 (1996)
  14. "A revised medium for rapidgrowth and bioassays with tobacco tissue cultures", pp.473~497 (1962)
  15. "Factors affecting Agrobacterium tumefaciensmediated transformation of peppermint", 19, pp.304~310 (2000)
  16. "High efficiency Agrobacterium-mediated transformation and regeneration of Citrus", 104, pp.183~191 (1995)
  17. "Identification of the signal molecules produced by woundedplant cells that activate T-DNA transfer in Agrobacteriumtumefaciens", pp.624~318 628 (1986)
  18. "Genetic transformation of Torenia fournieri L. mediated by Agrobacterium rhizogenes", 72, pp.211~216 (2006)
  19. "Environmental conditionsdifferentially affect vir gene induction in different Agrobacteriumstrains Role of the VirA sensor protein", pp.1051~1059 (1991)

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