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Proteomics approaches for the studies of bone metabolism

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Bone is an active tissue, in which bone formation by osteoblast is followed by bone resorption by osteoclasts, in arepeating cycle. Proteomics approaches may allow thedetection of changes in cell signal transduction, and theregulatory mechanism of cell differentiation. LC-MS/MS-basedquantitative methods can be used with labeling strategies,such as SILAC, iTRAQ, TMT and enzymatic labeling. Whenused in combination with specific protein enrichmentstrategies, quantitative proteomics methods can identifyvarious signaling molecules and modulators, and theirinteracting proteins in bone metabolism, to elucidatebiological functions for the newly identified proteins in thecellular context. In this article, we will briefly review recentmajor advances in the application of proteomics for bonebiology, especially from the aspect of cellular signaling.

Abstract

Bone is an active tissue, in which bone formation by osteoblast is followed by bone resorption by osteoclasts, in arepeating cycle. Proteomics approaches may allow thedetection of changes in cell signal transduction, and theregulatory mechanism of cell differentiation. LC-MS/MS-basedquantitative methods can be used with labeling strategies,such as SILAC, iTRAQ, TMT and enzymatic labeling. Whenused in combination with specific protein enrichmentstrategies, quantitative proteomics methods can identifyvarious signaling molecules and modulators, and theirinteracting proteins in bone metabolism, to elucidatebiological functions for the newly identified proteins in thecellular context. In this article, we will briefly review recentmajor advances in the application of proteomics for bonebiology, especially from the aspect of cellular signaling.

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