Lactoferrin Constitutively Enhances Differentiation of Osteoblastic MC3T3-E1 Cells in Vitro
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
During bone remodeling, there is requirement ofdifferentiation of osteoblastic cells. Previously, we identifiedproteins differentially expressed in soft tissue during bonehealing. Of these proteins, we focused the effect of LTF ondifferentiation of osteoblast. In order to analyze theosteogenic ability of LTF, we treated conditioned mediacollected from human LTF-stably transfected HEK293Tcells into osteoblastic MC3T3-E1. The results showed thatthe activity and expression of alkaline phosphatase wereincreased in MC3T3-E1 cells treated with conditioned mediacontaining LTF in dose- and time-dependent manner. At thesame time, we observed the significant increase of theexpression of osteoblastic genes, such as ALP, BSP,COL1A1, and OCN, and along with matrix mineralizationgenes, such as DMP1 and DMP2, in LTF conditionedmedia-treated groups. Moreover, the result of treatingrecombinant human LTF directly into osteoblasticMC3T3-E1 showed the same pattern of treating conditionedmedia containing LTF. Our study demonstrated that LTFconstitutively enhances osteoblastic differentiation via induction of osteoblastic genes and activation of matrix mineralization in MC3T3-E1 cells.
Abstract
During bone remodeling, there is requirement ofdifferentiation of osteoblastic cells. Previously, we identifiedproteins differentially expressed in soft tissue during bonehealing. Of these proteins, we focused the effect of LTF ondifferentiation of osteoblast. In order to analyze theosteogenic ability of LTF, we treated conditioned mediacollected from human LTF-stably transfected HEK293Tcells into osteoblastic MC3T3-E1. The results showed thatthe activity and expression of alkaline phosphatase wereincreased in MC3T3-E1 cells treated with conditioned mediacontaining LTF in dose- and time-dependent manner. At thesame time, we observed the significant increase of theexpression of osteoblastic genes, such as ALP, BSP,COL1A1, and OCN, and along with matrix mineralizationgenes, such as DMP1 and DMP2, in LTF conditionedmedia-treated groups. Moreover, the result of treatingrecombinant human LTF directly into osteoblasticMC3T3-E1 showed the same pattern of treating conditionedmedia containing LTF. Our study demonstrated that LTFconstitutively enhances osteoblastic differentiation via induction of osteoblastic genes and activation of matrix mineralization in MC3T3-E1 cells.
참고문헌 (17)
- Zuo C, "Osteoblastogenesis regulation signals in bone remodeling", Osteoporos Int, 23, pp.1653~1663 (2012)
- Hadjidakis DJ, "Bone remodeling", Ann N Y Acad Sci, 1092, pp.385~396 (2006)
- Ward PP, "Multifunctional roles of lactoferrin : a critical overview", Cell Mol Life Sci, 62, pp.2540~2548 (2005)
- Teng CT, "Lactoferrin gene expression and regulation : an overview", Biochem Cell Biol, 80, pp.7~16 (2002)
- Oh SM, "Human neutrophil lactoferrin trans-activates the matrix metalloproteinase 1gene through stress-activated MAPK signaling modules", J Biol Chem, 276, pp.42575~42579 (2001)
- Grey A, "The low-density lipoprotein receptor-related protein 1 is a mitogenic receptor for lactoferrin in osteoblastic cells", Mol Endocrinol, 18, pp.2268~2278 (2004)
- Oh SM, "Neutrophil lactoferrin upregulates the human p53 gene through induction of NF-kappaB activation cascade", Oncogene, 23, pp.8282~8291 (2004)
- Guo HY, "Orally administered lactoferrin preserves bone mass and microarchitecture in ovariectomized rats", J Nutr, 139, pp.958~964 (2009)
- Yamano E, "Inhibitory effects of orally administrated liposomal bovine lactoferrin on the LPS-induced osteoclastogenesis", Lab Invest, 90, pp.1236~1246 (2010)
- Du M, "Protective effects of bovine colostrum acid proteins on bone loss of ovariectomized rats and the ingredients identification", Mol Nutr Food Res, 55, pp.220~228 (2011)
- Takayama Y, "Effect of lactoferrin-embedded collagen membrane on osteogenic differentiation of human osteoblast-like cells", J Biosci Bioeng, 107, pp.191~195 (2009)
- Yang HY, "Proteomic analysis of gingival tissue and alveolar bone during alveolar bone healing", Mol Cell Proteomics, 12, pp.2674~2688 (2013)
- Naot D, "Lactoferrin-a novel bone growth factor", Clin Med Res, 3, pp.93~101 (2005)
- Cornish J, "Lactoferrin is a potent regulator of bone cell activity and increases bone formation in vivo", Endocrinology, 145, pp.4366~4374 (2004)
- Khosla S, "Pathophysiology of age-related bone loss and osteoporosis", Endocrinol Metab Clin North Am, 34, pp.1015~1030 (2005)
- Marie PJ, "Osteoblasts in osteoporosis : past, emerging, and future anabolic targets", Eur J Endocrinol, 165, pp.1~10 (2011)
- Riggs BL, "Drugs used to treat osteoporosis : the critical need for a uniform nomenclature based on their action on bone remodeling", J Bone Miner Res, 20, pp.177~184 (2005)