Mycobacterium abscessus MAB2560 induces maturation of dendritic cells via Toll-like receptor 4 and drives Th1 immune response
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In this study, we showed that Mycobacterium abscessusMAB2560 induces the maturation of dendritic cells (DCs),which are representative antigen-presenting cells (APCs). M. abscessus MAB2560 stimulate the production of pro-inflammatorycytokines [interleukin (IL)-6, tumor necrosis factor (TNF)-α,IL-1β, and IL-12p70] and reduce the endocytic capacity andmaturation of DCs. Using TLR4-/- DCs, we found that MAB2560mediated DC maturation via Toll-like receptor 4 (TLR4). MAB2560 also activated the MAPK signaling pathway, whichwas essential for DC maturation. Furthermore, MAB2560-treated DCs induced the transformation of naïve T cells topolarized CD4+ and CD8+ T cells, which would be crucial forTh1 polarization of the immune response. Taken together, ourresults indicate that MAB2560 could potentially regulate thehost immune response to M. abscessus and may have criticalimplications for the manipulation of DC functions fordeveloping DC-based immunotherapy.
Abstract
In this study, we showed that Mycobacterium abscessusMAB2560 induces the maturation of dendritic cells (DCs),which are representative antigen-presenting cells (APCs). M. abscessus MAB2560 stimulate the production of pro-inflammatorycytokines [interleukin (IL)-6, tumor necrosis factor (TNF)-α,IL-1β, and IL-12p70] and reduce the endocytic capacity andmaturation of DCs. Using TLR4-/- DCs, we found that MAB2560mediated DC maturation via Toll-like receptor 4 (TLR4). MAB2560 also activated the MAPK signaling pathway, whichwas essential for DC maturation. Furthermore, MAB2560-treated DCs induced the transformation of naïve T cells topolarized CD4+ and CD8+ T cells, which would be crucial forTh1 polarization of the immune response. Taken together, ourresults indicate that MAB2560 could potentially regulate thehost immune response to M. abscessus and may have criticalimplications for the manipulation of DC functions fordeveloping DC-based immunotherapy.
참고문헌 (25)
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