The Mycobacterium avium subsp. Paratuberculosis protein MAP1305 modulates dendritic cell-mediated T cell proliferation through Toll-like receptor-4
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In this study, we show that Mycobacterium avium subsp. paratuberculosis MAP1305 induces the maturation of bonemarrow-derived dendritic cells (BMDCs), a representativeantigen presenting cell (APC). MAP1305 protein induces DCmaturation and the production of pro-inflammatory cytokines(Interleukin (IL)-6), tumor necrosis factor (TNF)-α, and IL-1β)through Toll like receptor-4 (TLR-4) signaling by directly bindingwith TLR4. MAP1305 activates the phosphorylation of MAPKs,such as ERK, p38MAPK, and JNK, which is essential for DCmaturation. Furthermore, MAP1305-treated DCs transform naïveT cells to polarized CD4+ and CD8+ T cells, thus indicating akey role for this protein in the Th1 polarization of the resultingimmune response. Taken together, M. avium subsp. paratuberculosisMAP1305 is important for the regulation of innateimmune response through DC-mediated proliferation of CD4+and CD8+ T cells.
Abstract
In this study, we show that Mycobacterium avium subsp. paratuberculosis MAP1305 induces the maturation of bonemarrow-derived dendritic cells (BMDCs), a representativeantigen presenting cell (APC). MAP1305 protein induces DCmaturation and the production of pro-inflammatory cytokines(Interleukin (IL)-6), tumor necrosis factor (TNF)-α, and IL-1β)through Toll like receptor-4 (TLR-4) signaling by directly bindingwith TLR4. MAP1305 activates the phosphorylation of MAPKs,such as ERK, p38MAPK, and JNK, which is essential for DCmaturation. Furthermore, MAP1305-treated DCs transform naïveT cells to polarized CD4+ and CD8+ T cells, thus indicating akey role for this protein in the Th1 polarization of the resultingimmune response. Taken together, M. avium subsp. paratuberculosisMAP1305 is important for the regulation of innateimmune response through DC-mediated proliferation of CD4+and CD8+ T cells.
참고문헌 (23)
- Banchereau, J., "Immunobiology of dendritic cells", Annu. Rev. Immunol, 18, pp.767~811 (2000)
- Alvarez, D., "Mechanisms and consequences of dendritic cell migration", Immunity, 29, pp.325~342 (2008)
- Mosmann, T. R., "Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins", J. Immunol, 136, pp.2348~2357 (1986)
- Liu, Y. J., "Dendritic cell subsets and lineages, and their functions in innate and adaptive immunity", Cell, 106, pp.259~262 (2001)
- Lipscomb, M. F., "Dendritic cells: immune regulators in health and disease", Physiol. Rev, 82, pp.97~130 (2002)
- Hermon-Taylor, J., "Protagonist. Mycobacterium avium subspecies paratuberculosis is a cause of Crohn's disease", Gut, 49, pp.755~756 (2001)
- Abraham, C., "Inflammatory bowel disease", N. Engl. J. Med, 361, pp.2066~2078 (2009)
- Lee, J. S., "Mycobacterium avium subsp. paratuberculosis fibronectin attachment protein activates dendritic cells and induces a Th1 polarization", Infect. Immun, 77, pp.2979~2988 (2009)
- Hoek, A., "Epitopes of Mycobacterium avium ssp. paratuberculosis 70kDa heat-shock protein activate bovine helper T cells in outbred cattle", Vaccine, 28, pp.5910~5919 (2010)
- Basagoudanavar, S. H., "Cellular immune responses to 35 kDa recombinant antigen of Mycobacterium avium paratuberculosis", Vet. Res. Commun, 30, pp.357~367 (2006)
- Mogensen, T. H., "Pathogen recognition and inflammatory signaling in innate immune defenses", Clin. Microbiol. Rev, 22, pp.240~273 (2009)
- Akira, S., "Toll-like receptors: critical proteins linking innate and acquired immunity", Nat. Immunol, 2, pp.675~680 (2001)
- Luger, R., "Toll-like receptor 4 engagement drives differentiation of human and murine dendritic cells from a pro- into an anti-inflammatory mode", PLoS One, 8, pp.e54879~ (2013)
- Means, T. K., "Human toll-like receptors mediate cellular activation by Mycobacterium tuberculosis", J. Immunol, 163, pp.3920~3927 (1999)
- Rescigno, M., "Dendritic cell survival and maturation are regulated by different signaling pathways", J. Exp. Med, 188, pp.2175~2180 (1998)
- Elftman, M. D., "Corticosterone impairs dendritic cell maturation and function", Immunology, 122, pp.279~290 (2007)
- Takeda, K., "Toll-like receptors in innate immunity", Int. Immunol, 17, pp.1~14 (2005)
- Jung, I. D., "Enhanced efficacy of therapeutic cancer vaccines produced by co-treatment with Mycobacterium tuberculosis heparin-binding hemagglutinin, a novel TLR4 agonist", Cancer Res, 71, pp.2858~2870 (2011)
- Lei, L., "Limited phenotypic and functional maturation of bovine monocyte-derived dendritic cells following Mycobacterium avium subspecies paratuberculosis infection in vitro", Vet. Immunol. Immunopathol, 120, pp.177~186 (2007)
- Noh, K. T., "Protein kinase C delta (PKCdelta)-extracellular signal-regulated kinase 1", 2 (ERK1, J. Biol. Chem, pp.2012, 287[DOI]
- 노경태, "The Mycobacterium avium subsp. paratuberculosis fibronectin attachment protein, a toll-like receptor 4 agonist, enhances dendritic cell-based cancer vaccine potency", Experimental and Molecular Medicine, 44(5), pp.340~349 (2012), 생화학분자생물학회
- 박민철, "CD83 expression induced by CpG-DNA stimulation in a macrophage cell line RAW 264.7", BMB Reports, 46(9), pp.448~453 (2013), 생화학분자생물학회
- 지향, "Connexin32 inhibits gastric carcinogenesis through cell cycle arrest and altered expression of p21Cip1 and p27Kip1", BMB Reports, 46(1), pp.25~30 (2013), 생화학분자생물학회