PER3, a novel target of miR-103, plays a suppressive role in colorectal cancer in vitro
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Colorectal cancer has become the third most common cancerand leads to high mortality worldwide. Although colorectalcancer has been studied widely, the underlying molecularmechanism remains unclear. PER3 is related to tumordifferentiation and the progression of colorectal cancer. Highexpression of miR-103 is associated with poor prognosis inpatients with colorectal cancer. However, the relationshipbetween miR-103 and PER3 in CRC cells remains unclear. Inthis study, we found that PER3 was downregulated in CRCtissues and CRC cell lines, whereas miR-103 was upregulatedin CRC cell lines. We also found that PER3 promoted CRCcells apoptosis. These results indicate that PER3 plays asuppressive role in CRC cells. Moreover, we found that PER3was targeted, at least partially, by miR-103. Taken together, weprovide evidence to characterize the role of PER3 in CRC, which may be a new therapeutic target for CRC.
Abstract
Colorectal cancer has become the third most common cancerand leads to high mortality worldwide. Although colorectalcancer has been studied widely, the underlying molecularmechanism remains unclear. PER3 is related to tumordifferentiation and the progression of colorectal cancer. Highexpression of miR-103 is associated with poor prognosis inpatients with colorectal cancer. However, the relationshipbetween miR-103 and PER3 in CRC cells remains unclear. Inthis study, we found that PER3 was downregulated in CRCtissues and CRC cell lines, whereas miR-103 was upregulatedin CRC cell lines. We also found that PER3 promoted CRCcells apoptosis. These results indicate that PER3 plays asuppressive role in CRC cells. Moreover, we found that PER3was targeted, at least partially, by miR-103. Taken together, weprovide evidence to characterize the role of PER3 in CRC, which may be a new therapeutic target for CRC.
참고문헌 (22)
- Gery, S., "The role of circadian regulation in cancer", Cold. Spring. Harb. Symp. Quant. Biol, 72, pp.459~464 (2007)
- Buhr, E. D, "Molecular components of the Mammalian circadian clock", Handb. Exp. Pharmacol, 217, pp.3~27 (2013)
- Wang, X., "Reduced expression of PER3 is associated with incidence and development of colon cancer", Ann. Surg. Oncol, 19, pp.3081~3088 (2012)
- Matsuo, T., "Control mechanism of the circadian clock for timing of cell division in vivo", Science, 302, pp.255~259 (2003)
- Calin, G. A., "MicroRNA signatures in human cancers", Nat. Rev. Cancer, 6, pp.857~866 (2006)
- Yu, D., "microRNA-103 regulates the growth and invasion of endometrial cancer cells through the downregulation of tissue inhibitor of metalloproteinase 3", Oncol. Lett, 3, pp.1221~1226 (2012)
- Annibali, D., "A new module in neural differentiation control: two microRNAs upregulated by retinoic acid, miR-9 and -103, target the differentiation inhibitor ID2", PLoS One, 7, pp.e40269~ (2012)
- Chen, H. Y., "miR-103", 107 promote metastasis of colorectal cancer by targeting the metastasis suppressors DAPK and KLF4, 2012, pp.72, 3631~3641
- Sahar, S., "Metabolism and cancer: the circadian clock connection", Nat. Rev. Cancer, 9, pp.886~896 (2009)
- Zhu, Y., "Testing the circadian gene hypothesis in prostate cancer: a population-based case-control study", Cancer Res, 69, pp.9315~9322 (2009)
- Sato, F., "PERIOD1 (PER1) has anti-apoptotic effects, and PER3 has pro-apoptotic effects during cisplatin (CDDP) treatment in human gingival cancer CA9-22 cells", Eur. J. Cancer, 47, pp.1747~1758 (2011)
- Mazzoccoli, G., "Clock gene expression levels and relationship with clinical and pathological features in colorectal cancer patients", Chronobiol. Int, 28, pp.841~851 (2011)
- Gaziel-Sovran, A., "miR-30b", 30d regulation of GalNAc transferases enhances invasion and immunosuppression during metastasis, 2011, pp.20, 104~118
- Xu, Y., "MicroRNA- 335 acts as a metastasis suppressor in gastric cancer by targeting Bcl-w and specificity protein 1", Oncogene, 31, pp.1398~1407 (2012)
- Liu, M., "miR-137 targets Cdc42 expression, induces cell cycle G1 arrest and inhibits invasion in colorectal cancer cells", Int. J. Cancer, 128, pp.1269~1279 (2011)
- Asangani, I. A., "MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer", Oncogene, 27, pp.2128~2136 (2008)
- Weber, D. G., "Identification of miRNA-103 in the cellular fraction of human peripheral blood as a potential biomarker for malignant mesothelioma--a pilot study", PLoS One, 7, pp.e30221~ (2012)
- Niquet, J., "Bim, Bad, and Bax: a deadly combination in epileptic seizures", J. Clin. Invest, 113, pp.960~962 (2004)
- Danial, N. N., "Cell death: critical control points", Cell, 116, pp.205~219 (2004)
- Shapiro, G. I., "Cyclin-dependent kinase pathways as targets for cancer treatment", J. Clin. Oncol, 24, pp.1770~1783 (2006)
- Xu, Y., "Induction of genetic instability by gain-offunction p53 cancer mutants", Oncogene, 27, pp.3501~3507 (2008)
- Vousden, K. H., "p53 in health and disease", Nat. Rev. Mol. Cell Biol, 8, pp.275~283 (2007)