치면열구전색제의 광중합 후 표면 처리 방법에 따른미반응 모노머 용리 비교
Comparison of monomer release from pit and fissure sealant following various surface treatment
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초록
본 연구에서는 치면열구전색제 중합 후 표면의 미반응 모노머 층을 임상에서 이용할 수 있는 다양한 방법으로 제거하고, 고성능 액체 크로마토그래피를 이용하여 미반응 모노머를 정량분석 함으로써 미반응 모노머의 제거효과를 비교 평가하고자 하였다. 표면처리 방법에 따라 광중합 후 표면을 어떤 처리도 시행하지 않은 군을 대조군, 증류수로 10초간 세척한 군을 I군(Group I), 75% 알콜에 젖은 면구로 10초간 문지른 군을 II군(Group II), 퍼미스와 러버컵을 이용하여 10초간 처리하고 증류수로 가볍게 세척한 군을 III군(Group III)으로 분류하였다. 표면처리 후 각각의 시편을 3차 증류수에 넣은 후 37℃ 항온수조에서 10분간 보관한 후 각각의 용리액을 HPLC를 이용하여 정성 및 정량분석하여 다음과 같은 결과를 얻었다. 1. 표면처리를 하지 않은 군과 표면처리를 시행한 모든 군에서 TEGDMA를 제외한 어떠한 모노머들도 검출되지 않았다. 2. 표면처리를 한 모든 군은 표면처리를 하지 않은 군에 비하여, 용리되는 모노머량이 유의할 만하게 감소하였다(P<0.05). 3. 퍼미스와 러버컵을 이용해 표면처리한 군에서 잔존 모노머 제거효과가 다른 군에 비해 유의성 있게 높았다(P<0.05).
Abstract
The aim of this study was comparison of effectiveness of surface treatment methods in reducing the oxygen-inhibited layer of a commercially available freshly polymerized, light cured dental sealant(concise™, 3M, St Paul, USA). Surface treatment groups were consisted of no treatment(negative control group) and 3 experimental groups according to surface treatment of light-cured sealant. Experimental group Ⅰ was 10 seconds' exposure to distilled water syringe, group Ⅱ was 10 seconds' manual application using a cotton pellet wetted with 75% alcohol and group Ⅲ was 10 seconds' prophylaxis with pumice/water slurry using rubber cup on a slow-speed handpiece. All specimens were immersed in 5㎖ distilled water and stored at 37c water bath for 10 minutes. All eluates were analyzed by HLPC for identification and quantitive analysis of monomers. The results of this study can be summarized as follows. 1. None of the chromatograms of the tested sealant displayed peaks with the same retention time as that of the standard solution, except for TEGDMA. 2. All surface treatment group had a statistically significant decrease of monomer release in comparison with no treatment group. 3. Removal effects of unreacted monomer in group Ⅲ was statistically significant in comparison with group Ⅰ and group Ⅱ. These results revealed that mechanical method using pumice and rubber cup is the most effective in removing residual monomer and may be valuable to be used effectively in clinic.
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