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Streptococcus mutans GS5의 세포막 단백질 Ag Ⅰ/Ⅱ에 대한 단항체의 생산

Generation of a monoclonal antibody against AgI/II, a cellularsurface protein of Streptococcus mutans GS5

한지혜|김재곤|양연미|백병주|전철완
대한소아치과학회 (The Korean Academy Of Pediatric Dentistry)|2006.11|대한소아치과학회지 (Journal of the Korean academy of pediatric dentistry)|제33권 제4호|pp. 587-596

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참고문헌

키워드

Streptococcus mutansMonoclonal antibody연쇄상 구균Antigen I/II단항체
주제분류치의학

초록

치아우식증의 원인균으로 알려져 있는 Streptococcus mutans의 여러 균주 중 GS-5 균주는 AgI/II 유전자 내에 생기는 nonsense mutation에 의하여 절편의 분비형 AgI/II 단백질을 발현한다. 이러한 사실은 S. mutans GS-5가 독특한 임상 기능을 가질 수 있음을 암시하며, 최근의 보고는 임상적으로 분리된 S. mutans 균주를 이용한 실험 결과에 근거하여 이러한 가능성을 지지한다. 본 연구는 이전의 실험을 통하여 S. mutans의 agI/II 유전자를 확보한 후 재조합 단백질인 N-terminal AgI/II 단백질을 생산하였다. 이 후 하이브리도마를 통하여 1C11A라 명명되는 세포막 단백질 AgI/II에 대한 단항체를 생산하였으며, Western blot과 ELISA를 통하여 이 항체가 AgI/II 단백질에 매우 높은 특이성을 나타냄을 알 수 있었다. 새로이 얻어진 단항체는 AgI/II 와 관련된 질환의 기전을 규명하는데 기초 재료가 될 것이다.

Abstract

Most of oral streptococci express the Antigen I/II (AgI/II) proteins, cell wall anchored adhesions. AgI/II protein binds to salivary agglutinin glycoprotein, a component of tooth pellicle and to ligands in other bacteria. These associations play important roles in bacterial colonization. Recently, it was reported that diverse host molecules also interact with AgI/II protein and that these interactions induce inflammatory responses from host cells. Among mutans streptococci containing -type hemolytic activity, Streptococcus mutans is a causative agent for dental caries. Compared with many other strains of S. mutans, GS-5 strain is unique in that this bacterium expresses truncated secretory AgI/II protein due to the nonsense mutation in the agI/II gene. This indicates that S. mutans GS-5 has a different clinical role and a recent report supported this idea based on the results from clinically isolated S. mutans strains. Previously, we had cloned agI/II gene from S. mutans GS-5 and generated recombinant N-terminal AgI/II protein. In this study, we further produced a hybridoma line expressing anti-AgI/II monoclonal antibodies named as 1C11A. This antibody showed high sensitivity to AgI/II protein in Western blot and ELISA. This new reagent will provide a basis for investigating the mechanisms of AgI/II-related diseases.

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관련 논문AI추천

연쇄상구균(Streptococcus mutans GS-5)의 항원단백질 AgI/II의N-terminus절편에 대한 항체형성김재곤, 박정렬, 백병주, 양연미, 한지혜대한소아치과학회 · 대한소아치과학회지2006 · 제33권 제3호연쇄상구균 GS-5의 Ag I/II와 gtfD유전자 클로닝김재곤, 백병주, 서정아, 양연미, 정진우대한소아치과학회 · 대한소아치과학회지2005 · 제32권 제2호단일클론항체를 사용한 타액 내 Streptococcus mutans 검출김미아, 김재곤, 백병주, 양연미, 이경열, 이대우, 이민정대한소아치과학회 · 대한소아치과학회지2015 · 제42권 제1호Streptococcus mutans GS-5 Glucosyltransferase의 클로닝과 발현김수경, 김재곤, 박정렬, 백병주, 양연미, 이경열대한소아치과학회 · 대한소아치과학회지2008 · 제35권 제1호소아와 성인의 타액 내 AgⅠ/Ⅱ 특이 IgA 와 우식경험도의 관계김수경, 김재곤, 백병주, 양연미, 이경열, 임수민대한소아치과학회 · 대한소아치과학회지2008 · 제35권 제4호단클론항체를 이용한 타액 내 Streptococcus mutans 수준의 측정김미아, 김재곤, 김추성, 백병주, 양연미, 이경열, 임수민대한소아치과학회 · 대한소아치과학회지2010 · 제37권 제2호

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