Real-time PCR을 이용한 치주질환 원인균의 정량적 분석
Quantitative detection of periodontopathic bacteria using real-time PCR
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Real-time PCR 기법을 이용하여 구강내 치태에서 특정 세균의 존재여부를 알 수 있을 뿐 아니라 정량적 분석이 가능하게 되었다. 이 연구는 real-time PCR 기법을 이용하여 8-18세의 청소년에서 치주질환 원인균의 조성을 알아보고자 전신질환이 없는 어린이 및 청소년 65명을 대상으로 치은연하의 치태를 채취하여 5종의 치주질환 원인균의 출현율과 그 양을 측정하고, 또한 함께 측정한 치태지수 및 치은지수와의 상관관계를 조사하여 다음과 같은 결론을 얻었다. 1. 치태지수의 평균은 1.33이었으며, 치은지수의 평균은 0.97이었다. 치태지수는 나이가 증가할수록 낮아졌다(p<0.05). 2. 치주질환 원인균의 출현율은 P. gingivalis 61.5%, T. forsythia 53.8%, T. denticola 29.2%, A. actinomycetemcomitans 15.4%, F. nucleatum은 100%였다. P. gingivalis, T. denticola는 10세 이후 출현율이 증가하였고 A. actinomycetemcomitans는 8-10세 군에서 출현율이 높았다(p<0.05). 3. 정량적 분석에서 치태지수와 치은지수는 F. nucleatum의 양과 유의한 상관성이 있었으며, 치태지수는 치은지수와 유의한 상관성이 있었다. T. forsythia는 A. actinomycetemcomitans, T. denticola양과 강한 상관성을 보였으며, T. denticola양과 A. actinomycetemcomitans도 강한 상관관계를 가지고 있었다.
Abstract
The detection of special bacteria of oral plaque and quantitative analysis are possible through the real-time PCR technique. The aim of this study was to investigate the prevalence and the amount of five periodontopathic bacteria using real-time PCR, and evaluate the relationship among amount of bacteria, plaque index and gingival index. Sixty five subjects ranging from 8 to 18 years old were enrolled. Plaque index and gingival index were examined and five periodontopathic bacteria were surveyed in subgingival plaque samples. The results were analyzed by ANOVA and Pearson's correlation. Results: The prevalence of P. gingivalis was 61.5%, T. forsythia was 53.8%, T. denticola was 29.2%, A. actinomycetemcomitans was 15.4% and F. nucleatum was 100%. Plaque index and gingival index have a significant relationship with the amount of F. nucleatum. The amount of T. forsythia has a strong relationship with the amount of T. denticola and A. actinomycetemcomitans(p<0.05).
참고문헌 (40)
- Mandell RL, "A longitudinal microbiological investigation of Actinobacillus actinomycetemcomitans and Eikenella corrodens in juvenile periodontitis", InfectImmun, 45, pp.778~780 (1984)
- Haffajee AD, "Socransky SS : Microbial etiological agents of destructive periodontal diseases", Periodontol, 5, pp.78~111 (1994)
- Frisken KW, "Suspected periodontopathic microorganisms and their oral habitats in young children", Oral Microbiol Immunol, 2, pp.60~64 (1987)
- Delaney JE, "Microbiology of subgingival plaque from children with localized prepubertal periodontitis", Oral Microbiol Immunol, 2, pp.71~76 (1987)
- Slots J, "Detection of putative periodontal pathogens in subgingival specimens by 16S ribosomal DNA amplification with the polymerase chain reaction", Clin Infect Dis, 20(2), pp.304~307 (1995)
- Conrads G, "Reaction and dot-blot hybridization to monitor the distribution of oral pathogens within plaque samples of periodontally healthy individuals", J Periodontol, 67, pp.994~1003 (1996)
- Watanabe K, "Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Treponema denticola detection in oral plaque samples using the polymerase chain reaction", J Clin Periodontol, 23, pp.212~219 (1996)
- Okada M, "PCR detection of 5 putative periodontal pathogens in dental plaque samples from children 2 to 12 years of age", J Clin Periodontol, 28, pp.576~582 (2001)
- Sakamoto M, "Rapid detection and quantification of five periodontopathic bacteria by real-time PCR", Microbiol Immunol, 45, pp.39~44 (2001)
- Kawada M, "Prevalence of Porphyromonas gingivalis in relation to periodontal status assessed by real-time PCR", Oral Microbiol Immunol, 19, pp.289~292 (2004)
- K¨on¨onen E, "Development of oral bacterial flora in young children", Ann Med, 32, pp.107~112 (2000)
- Sweeny EA, "Prevalence and microbiology of localized prepubertal periodontitis", Oral Microbiol Immunol, 2, pp.65~70 (1987)
- Sj¨odin B, "A retrospective radiographic study of alveolar bone loss in the primary dentition in patients with localized juvenile periodontitis", J Clin Periodontol, 16, pp.125~127 (1989)
- Watanabe K, "Detection of Porphyromonas gingivalis in oral plaque samples by use of the polymerase chain reaction", J Dent Res, 72, pp.1040~1044 (1993)
- Silness J, "Periodontal disease in pregnancy. Correlation between oral hygiene and periodontal condition", Acta Odontol Scand, 22, pp.112~135 (1964)
- L¨oe H, "The gingival index, the plaque index and the retention index system", J Dent Res, 38, pp.610~616 (1927)
- Kato H, "Quantitative Mdetection of volatile sulfur compound producing microorganism in oral specimens using real-time PCR", Oral Dis, 11, pp.67~71 (2005)
- Slots J, "The predominant cultivable organisms in juvenile periodontitis", Scand J Dent Res, 84, pp.1~10 (1976)
- Slots J, "The predominant cultivable microflora of advanced periodontitis", Scand J Dent Res, 85, pp.114~121 (1997)
- Schroeder HE, "The structure of microbial dental plaque", pp.49~74 (1970), Dental plaque
- Listgarten MA, "Structure of the microbial flora associated With periodontal health and disease in man. A light and electron microscopic study", J Periodontol, 47, pp.1~18 (1976)
- Consensus report, "Anonymous. Periodontal diseases: Pathogenesis and microbial factors", Ann Periodontol, 1, pp.926~932 (1996)
- Slots J, "Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation", Infect Immun, 29, pp.1013~1020 (1980)
- Newman MG, "Studies of the microbiology of periodontosis", J Periodontol, 47, pp.373~379 (1976)
- Lopez NJ, "Occurrence of Actinobacillus actimomycetemcomitans, Porphyromons gingivalis and Prevotella intermedia in progressing adult periodontitis", J Periodontol, 71, pp.948~954 (2000)
- Papapanou PN, ""Checkerboard" assessments of periodontal microbiota and their antibody responses: a case control study", J Periodontol, 71, pp.885~987 (2000)
- Lai CH, "Bacteroides forsythus in adult gingivitis and periodontitis", Oral Microbiol and Immunol, 2, pp.152~157 (1987)
- Listgarten MA, "Electron microscopic observations of the bacterial flora of acute necrotizing ulcerative gingivitis", J Periodontol, 36, pp.328~339 (1965)
- Simonson LG, "Quantitative relationship of Treponema denticola to severity of periodontal disease", Infect Immun, 56, pp.726~728 (1988)
- Dzink JL, "Gramnegative species associated with active destructive periodontal lesions", J Clin Periodontol, 12, pp.648~659 (1985)
- Ashimoto A, "Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions", Oral Microbiol Immunol, 11, pp.266~273 (1996)
- Kimura S, "Periodontopathic bacterial infection in childhood", J Periodontol, 73, pp.20~26 (2002)
- Umeda M, "The distribution of periodontopathic bacteria among Japanese children and their parents", J Periodontol Res, 39, pp.398~404 (2004)
- 정우성, "어린이의 치은연하 치태내 치주질환 원인균의 출현율 조사" (2004), 경희대학교 대학원
- 김선미, "다운증후군 환자의 치주질환 원인균의 출현율", 대한소아치과학회지, 32(4), pp.717~725 (2005), 대한소아치과학회
- Suzuki N, "Quantitative microbiological study of subgingival plaque by realtime PCR shows correlation between levels of Tannerella forsythensis and Fusobacterium spp", J Clin Microbiol, 42, pp.2255~2257 (2004)
- Yoshida A, "Development of a 5' fluorogenic nuclease-based realtime PCR assay for quantitative detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis", J Clin Microbiol, 41, pp.863~866 (2003)
- Lyons SR, "Quantitative realtime PCR for Porphyromonas gingivalis and total bacteria", J Clin Microbiol, 38, pp.2362~2365 (2000)
- Asai Y, "Detection and quantification of oral treponemes in subgingival plaque by real-time PCR", J Clin Microbiol, 40, pp.3334~3340 (2002)
- Sawamoto Y, "Detection of periodontopathic bacteria and an oxidative stress marker in saliva from periodontitis patients", Oral Microbiol Immunol, 20, pp.216~220 (2005)
