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어린이 치면세균막에서 치주질환원인균과 항생제 내성유전자의 출현율

PERIODONTOPATHIC BACTERIA AND ANTIBIOTIC RESISTANCE GENES OF ORAL BIOFILMS IN CHILDREN

조성훈|이석우|오종석|김선미|강미선|최남기|임회순|임회정
대한소아치과학회 (The Korean Academy Of Pediatric Dentistry)|2011.05|대한소아치과학회지 (Journal of the Korean academy of pediatric dentistry)|제38권 제2호|pp. 170-178

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키워드

중합효소연쇄반응치주질환 원인균항생제 내성항생제 내성유전자치면세균막Periodontopathic bacteriaAntibiotic resistanceAntibiotic resistance determinantsOral biofilmPolymerase chain reaction
주제분류치의학

초록

항생제 사용에 따라 구강내 세균들이 가지게 되는 내성의 발현이 문제가 될 수 있다. 어린이 치면세균막에서 치주질환의 원인균들과 흔히 사용하고 있는 항생제들에 대한 내성유전자의 출현율을 알아보고자 중합효소연쇄반응을 이용하여 조사하였다. 1. 치주질환 원인균의 출현율은 F. nucleatum 95.4%, T. forsythia 55.2%이었으며, P. gingivalis 40.2%, A. actinomycetemcomitans 5.7%, T. denticola는 3.4% 순이었다. 2. 항생제 내성유전자의 출현율 조사에서 cephalosporin 분해효소인 cfxA는 100%에서 발견되었으며 β-lactam 분해효소인 blaTEM과 tetracycline 내성유전자인 tet(M)도 100%의 출현율을 보였다. tet(Q)는 88.5%, β-lactam 분해효소인blaSHV는 29.9%, macrolide계 내성 ermF유전자는 87.4%, vancomycin 내성 vanA는 48.5%의 출현율을 보였다. Aminoglycoside에 대한 복합 내성을 보이는 aacA-aphD와 meticillin 내성유전자 mecA는 9.2%로 가장 낮은 출현율을 보였다. 3. 치주질환 원인균과 항생제 내성유전자와의 관련성 조사에서 T. forsythia와 blaSHV간에 그리고 P. gingivalis와 vanA간에 유의한 상관성이 있었다. 항생제 내성유전자 tet(Q)와 ermF (0.514)간에 중등도의 상관성을 나타내었으며, mecA 와 vanA (0.25)간에 유의한 상관성을 나타내었다. 건강한 어린이들의 치면세균막에 다양한 치주질환 원인균들과 항생제 내성유전자들이 존재하며, 상호 관련성을 가지고 존재함을 보여주었다.

Abstract

The purpose of this study was to assess the prevalence of periodontopathic bacteria and antibiotic resistance determinants from oral biofilm of children. Subgingival dental plaque was isolated from 87 healthy children, and PCR was performed to determine the presence of 5 periodontal pathogens including P. gingivalis, T. forsythia,T. denticola, F. nucleatum, A. actinomycetemcomitans, and nine resistance genes including tet(Q), tet(M),ermF, aacA-aphD, cfxA, blaSHV, blaTEM, vanA, mecA. 1. The prevalence of F. nucleatum, T. forsythia, and P. gingivalis was 95.4%, 55.2%, and 40.2%, respectively. In addition, the prevalence of A. actinomycetemc omitans was 5.7%, while T. denticola was 3.4%. 2. In analysis of antibiotic resistance determinants, cfxA, blaTEM and tet(M) were detected in all the samples tested. It was also found that the prevalence of tet(Q) showing tetracycline resistance, blaSHV associated with resistance to β-lactams, ermF exhibiting erythromycin resistance, and, vanA resulting vancomycin resistance was 88.5%, 29.9% 87.4%, and 48.5%, respectively. The aacA-aphD gene showing resistance to aminoglycosides and mecA gene harboring methicillin resistance exhibited the lowest prevalence with 9.2%. 3. In a correlation analysis between periodontopathic pathogens and antibiotic resistance determinants, it was found that there was a significant correlation between T. forsythia and blaSHV. Also, P. gingivalis and vanA showed a correlation. Finally, tet(Q) and ermF showed a significant correlation (phi: 0.514) while mecA and vanA also showed a correlation(phi: 0.25).

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