구강 미생물 7종에 대한 멘톨과 불소치약의 항균 활성 비교 연구
A Study on Antibacterial Activity of Menthol against Oral Microbes via Comparison with Fluoride Toothpaste
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구강에는 600종 이상의 미생물이 존재하는 것으로 보고되고 있다. 알코올과 항생제 제품을 대체하여 천연물을 사용한 구강 위생 제제의 개발연구가 보고되고 있으나, 특정 성분들의 균주별 항균 활성 등에 관한 연구는 부족하다. 본 연구에서는 멘톨, 녹차 추출물, 불소치약, 플루오린화나트륨을 사용하여 구강 미생물 7종 –S. mutans, E. coli, B. subtilis, S. aureus, B. cereus, P. aeruginosa, L. brevis- 에 대한 항균효능을 확인하고자 하였다. 디스크 확산법을 통해 멘톨(200 mg/mL)은 구강 미생물 7종, 치약의 경우는 2종의 균주 -S. mutans, B. cereus- 에서 10.4~ 21.9 mm의 clear zone이 형성되는 것을 확인하였다. 불소치약과 멘톨의 복합 처리 시에는 구강 미생물 4종에서 증가된 항균 활성을 확인하였다. 또한 MIC 분석을 통해, 멘톨은 200~500 ㎍/mL의 농도에서 모든 균주에 대한 최소억제농도를 확인할 수 있었고 플루오린화나트륨의 경우 E. coli에서 40 ㎍/mL의 억제농도를 확인할 수 있었다. 결과적으로, 구강청결제 및 구강질환 항생물질의 대체소재로서 멘톨의 활용 가능성을 제시하고자 한다.
Abstract
More than 600 kinds of microorganisms exist in the oral cavity. Studies on the development of oral hygiene preparations using natural products as a substitute for alcohol and antibiotic products have been reported, but studies on the antibacterial activity of specific ingredients by strain are insufficient. In this study, menthol, green tea extract, fluoride toothpaste, and sodium fluoride were used against 7 kinds of oral microorganisms, S. mutans, E. coli, B. subtilis, S. aureus, B. cereus, P. aeruginosa, L. brevis. Using the disk diffusion method, It was confirmed that clear zones of 10.4 to 21.9 mm are formed in 7 types of oral microorganisms when menthol (200 mg/mL) is treated and 2 strains, S. mutans and B. cereus, when treated with fluoride toothpaste. When combined treatment with fluoride toothpaste and menthol, increased antibacterial activity was observed in four oral microbes. Through the MIC analysis, the minimum inhibitory concentration of menthol for all strains was confirmed at a concentration of 200-500 ㎍/mL, and the inhibitory concentration of sodium fluoride was confirmed at 40 ㎍/mL in E. coli. As a result, we suggest that the possibility of using menthol as a substitute for oral cleansers and antibiotics for oral diseases.
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