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지의류 추출물의 구강세균에 대한 항균효과

Antimicrobial activities of oral bacteria by lichen extracts

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Objectives : By investigating antimicrobial activity of natural extracts, identifying its usefulness as antibiotic material to oral bacteria. Methods : Antimicrobial activity tests of 25 natural extracts were implemented on 6 type strains alleged to cause dental caries and 10 clinical strains isolated and identified from dental caries. Results : Among medicinal plants, Coptis japonica, Scutellaria baicalensis, Dictamnus dasycarpus showed antimicrobial activity. Among lichens, 3 methanol extracts and 6 acetone extracts showed antimicrobial activity. Minimum Inhibitory Concentration(MIC) test of Usnea aurantiacoatra KoLRI 004184, an acetone extract of lichen with the highest antimicrobial activity, on 6 type strains involved in dental caries and 10 strains isolated from dental caries resulted as follows; Actinomyces oris 1041 12㎍/ml, for Corynebacterium durum 3151 13㎍/ml, for Rothia dentocariosa KCTC 3204T 14㎍/ml, for R. dentocariosa 911 15㎍/ml, for R. dentocariosa 1822 12㎍/ml, for Lactobacillus casei KACC 12413T12㎍/ml, for L. acidophilus KACC 12419T 16㎍/ml, for L. rhamnosus 2421 15㎍/ml, for Streptococcus mutans KCTC 3065T 15 ㎍/ml, for S. mutans 121 13㎍/ml, for Streptococcus oralis 2221 18㎍/ml, for S. salivarius KCTC 5512 14㎍/ml, for S. salivarius 122 12㎍/ml, for S. sanguinis KCTC 3284T 14㎍/ml, for S. sanguinis 912 14㎍/ml, for Neisseria sp. KEM232 12㎍/ml. Conclusions : Even a small amount of extract from lichen Usnea aurantiacoatra KoLRI 004184 showed very high level of antimicrobial activation against all 16 bacterial strains involved in dental caries.

Abstract

Objectives : By investigating antimicrobial activity of natural extracts, identifying its usefulness as antibiotic material to oral bacteria. Methods : Antimicrobial activity tests of 25 natural extracts were implemented on 6 type strains alleged to cause dental caries and 10 clinical strains isolated and identified from dental caries. Results : Among medicinal plants, Coptis japonica, Scutellaria baicalensis, Dictamnus dasycarpus showed antimicrobial activity. Among lichens, 3 methanol extracts and 6 acetone extracts showed antimicrobial activity. Minimum Inhibitory Concentration(MIC) test of Usnea aurantiacoatra KoLRI 004184, an acetone extract of lichen with the highest antimicrobial activity, on 6 type strains involved in dental caries and 10 strains isolated from dental caries resulted as follows; Actinomyces oris 1041 12㎍/ml, for Corynebacterium durum 3151 13㎍/ml, for Rothia dentocariosa KCTC 3204T 14㎍/ml, for R. dentocariosa 911 15㎍/ml, for R. dentocariosa 1822 12㎍/ml, for Lactobacillus casei KACC 12413T12㎍/ml, for L. acidophilus KACC 12419T 16㎍/ml, for L. rhamnosus 2421 15㎍/ml, for Streptococcus mutans KCTC 3065T 15 ㎍/ml, for S. mutans 121 13㎍/ml, for Streptococcus oralis 2221 18㎍/ml, for S. salivarius KCTC 5512 14㎍/ml, for S. salivarius 122 12㎍/ml, for S. sanguinis KCTC 3284T 14㎍/ml, for S. sanguinis 912 14㎍/ml, for Neisseria sp. KEM232 12㎍/ml. Conclusions : Even a small amount of extract from lichen Usnea aurantiacoatra KoLRI 004184 showed very high level of antimicrobial activation against all 16 bacterial strains involved in dental caries.

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