소아의 치면세균막에 존재하는 mutans streptococci의 분포
Distribution of mutans streptococci in dental plaque of children
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초록
한국인 소아의 치면세균막에 존재하는 mutans streptococci 종 및 생물형의 발현빈도와 치아우식경험지수와 상관관계를 알아보기 위하여, 조선대학교 치과병원에 내원한 12세 미만 113명의 소아 환자의 치아우식경험지수를 구하고, 이들의 상하악 유전치 및 유구치의 협면 및 설면의 치면세균막 샘플을 채취하여 mutans streptococci을 MSB 배지에서 선택적으로 분리하였다. 이들의 biotype을 알아보기 위해 생화학적 검사를 실하였고, 이들의 종 수준에서의 동정을 위해 dextranase 유전자를 표적으로 하는 중합효소연쇄반응을 시행하여 하였다. 113명의 환자 중에서 40명의 치면세균막에서 40 균주의 mutans streptococci이 검출되었다. 이들 중 생물형 제I형(45%)이 가장 많이 검출되었으며, 그 다음으로 제Ⅳ형(32.5%), 제Ⅱ형(15%), 제V형(5%), 제III형(2.5%) 순으로 검출되었다. 또한, 종 수준에서의 발현빈도를 알아본 결과 S. mutans가 69%, S. sobrinus는 31%였다. mutans streptococci 종 또는 생물형에 따른 환자의 치아우식경험지수간의 차이는 없는 것으로 조사되었다(p>0.05). 이상의 결과를 종합할 때, 한국인의 소아의 구강 내에 존재하는 mutans streptococci 종의 발현빈도는 S. mutans가 가장 높았으며, 생물형으로는 제Ⅰ형이 가장 빈번하게 검출되었다. 또한 이들과 치아우식경험지수간의 상관관계가 없는 것으로 조사되었으며(p>0.05), 이는 치아우식증이 세균학적 요소만이 아닌 기타 여러원인 요소에 의해 발병되는 다는 여러 연구 결과와 일치함을 알 수 있었다.
Abstract
The aim of this study is to survey the frequency of mutans streptococci species and biotypes isolated from dental plaque in Korean children and the relationship between species and biotypes of mutans streptococci and dft index. Dental plaques were collected from the anterior and molar teeth of upper and lower jaws in the subjects, aged below 12 years old. A dental examination was performed for dft (decayed, filled, total) with the WHO caries diagnostic criteria. The mutans streptococci from the sample were cultured selectively on mitis salivarius-bacitracine (MSB) agar plate. For biotyping of mutans streptococci, biochemical test was performed. From the culture, bacterial genomic DNA was prepared for using of PCR template for the identification of mutans streptococci species. Forty strains of mutans streptococci were cultured from 40 patients. The biotype I (45%) and biotype IV (32.5%) were most frequently detected. The prevalence of S. mutans and S. sobrinus was 69% and 31%, respectively. There was no positive relationship between species and biotypes of mutans streptococci and dft index. Our results revealed that biotype I and S. mutans were frequently detected in Korean children and support that dental caries incidents by many causative factors not only bacterial factor.
참고문헌 (27)
- "A review of dental research using germfree animals", 78, pp.285~289 (1959)
- "Current classification of the oral streptococci", 13, pp.195~216 (1998)
- "Isolation and serotyping of Streptococcus mutans from teeth and feces of children", 11, pp.314~318 (1980)
- "Caries prevalence and salivary mutans streptococci in 0-2-year-old children of Japan", 19, pp.151~154 (1991)
- "A selective medium for Streptococcus mutans", 18, pp.1357~1364 (1973)
- "Cultural methods for the identification and quantitation of Streptococcus mutans and lactobacilli in oral samples", 1, pp.23~30 (1986)
- "Direct detection of Streptococcus mutans in human dental plaque by polymerase chain reaction", 11, pp.294~298 (1996)
- "PCR for detection and identification of Streptococcus sobrinus", 49, pp.1069~1074 (2000)
- "Detection of Streptococcus mutans by PCR amplification of spaP gene", 41, pp.231~235 (1994)
- "Identification of mutans streptococcal species by the PCR products of the dex genes", 46, pp.99~105 (2001)
- "A biochemical scheme for the separation of the five varieties of Streptococcus mutans", pp.1079~1081 (1974)
- "Let your antibody work-immunize early", 55 (1976)
- "The isolation of characterization of Streptococcus mutans serotype h from dental plaque of monkeys", pp.271~279 (1981)
- "Demonstration of five serological groups of streptococcal strains resembling streptococcus mutans", 21, pp.143~152 (1970)
- "Relationship between the caries experience of Korean school children and the distribution of Streptococci mutans in dental plaque", 18, pp.11~21 (1983)
- "Survey of the seven serological types of Streptococcus mutans in six-year-old children", 56, pp.1421~ (1977)
- "Occurrence of Streptococcus mutans and Streptococcus sanguis in the oral cavity and feces of young children microbial aspect of dental caries special supplement to Microbiology Abstracts"
- "Streptococcus mutans serotypes in young schoolchildren", 11, pp.141~152 (1977)
- "Biology,immunology and cariogenicity of Streptococcus mutans", 44, pp.331~384 (1980)
- "Longitudinal survey of the distribution of various serotypes of Streptococcus mutans in infants", 10, pp.497~502 (1979)
- "Prevalence of Streptococcus mutans serotypes Actinomyces and other bacteria in the plaque of children", 59, pp.1581~1589 (1980)
- "Predominant plaque flora of Tanzanian children exposed to high and low water fluoride concentrations", 13, pp.330~343 (1979)
- "Enumeration of mutans streptococci in clinical samples by using monoclonal antibodies", 28, pp.2467~2472 (1990)
- "Close association between Streptococcus sobrinus in the saliva of young children and smooth-surface caries increment", 27, pp.292~297 (1993)
- "Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction", 15, pp.258~262 (2000)
- "Biochemical and serological properties of Streptococcus mutans from various human and animal sources", 82, pp.357~370 (1974)
- "A biochemical scheme for the separation of the five varieties of Streptococcus mutans", 19, pp.1079~1081 (1974)