Preparation and characterization of hydroxyapatite from portunus sanguinolentus crab shell

Abstract

Hydroxyapatite a bio-ceramic is one of the main compounds present in our body tissues. Generally, bones have a high calciumcontent and Arthritis, Fracture, Infection, Tumours are the main factors that affect bones directly. Presently, steel plates areused in bone replacement surgeries. Natural hydroxyapatite and bones have similar physical and chemical characteristics andthis thus makes it biocompatible. Hydroxyapatite can be synthetically derived from crab shells, eggshells and fishbone. Amongst them, crab shells have a high calcium content and as a huge amount of crab shells are produced daily, the same iseasily available. Additionally, the cost of crab shells is less and hence, the shells can be used effectively for derivinghydroxyapatite (HA) powder synthetically. A huge quantity of Poryunus Sanguinolentus crab shells is available in and aroundRameshwaram coastal area. In this investigation, the Poryunus Sanguinolentus crab shell is used for HA preparation. Thereactions between Poryunus Sanguinolentus crab shell powder (C8H1305N) and calcium pyrophosphate (Ca2P2O7) wereperformed through grinding, followed by the heat treatment to produce Hydroxy Apatite (HA). Further, the physical andchemical characteristics of the HA were analysed and the results derived from the Scanning Electron Microscope (SEM)micrograph, Energy Dispersive X-Ray Analysis (EDAX), X-Ray Diffraction (XRD) analysis and Fourier Transform InfraredSpectroscopy analysis (FTIR), Biocompatibility analysis, Compressive strength and Tensile strength analysis had shown thathence HA can be produced from Poryunus Sanguinolentus crab shells. These results show that the HA powder derived fromthe shells can be used as a biocompatible material.