본문 바로가기
KERD
논문KCI 등재

향사양위탕이 시스플라틴 유발 랫트의 위장관 기능 장애에 미치는 영향

Beneficial Effect of Hyangsayangwi-tang on the Cisplatin-Induced Gastrointestinal Dysfunctions in Rats

0

조회

0

다운로드

2

인용

0

참고문헌

키워드

주제분류한의학

초록

Objectives This study aimed to observe the effect of Hyangsayangwi-tang on the cisplatin-induced gastrointestinal dysfunctions in rats. Methods Four groups(each of 8 rats per group) were used in this study. Saline and distilled water treated control rats are Intact vehicle control group. Delayed gatrointestinal mortility was induced by intraperitoneal treatment of cisplatin 2㎎/㎏, once a week for 5 weeks(Cisplatin control group). Hyangsayangwi-tang aqueous extracts(HY) were orally administered in a volume of 5㎖/㎏, once a day for 14 days from 4th ciplatin treatmernt(HY group). Ondansetron 1㎎/㎏ was subcutaneously treated, in a volume of 1㎖/㎏, as same as HY(ondansetron group). We measured the body weights, intestinal charcoal transit ratio, fecal parameters, fundus MDA, GSH contents and SOD, CAT activities, TPH and MAO activities, pyloric gastrin and serotonin contents with their immunoraective cells, colonic serotonin-immunoreactive cells, the histopathology of pylorus, fundus mucosa and colon. Results and Conclusions (1) The body weight gains, the small intestinal charcoal transfer rates, the fecal parameters(numbers, weights and water contents) were increased in HY, ondansetron group. (2) The inhibit of fundus antioxidant defense systems by cisplatin were decreased in HY, ondansetron group. (3) The pyloric TPH activities were increased and the pyloric MAO activities were decreased in HY group. (4) The pyloric gastric contents and the gastrin-immunoreactive cells were increased in HY group. And the pyloric serotonin contents and the pyloric and colonic serotonin-immunoreactive cells were decreased in HY group. (5) The pyloru atrophic changes and the gastric surface erosive damage regions by cisplatin were favorably inhibited by treatment of HY.HY, a representative Soeumin prescription improve GI dysfunctions and constipation retarded by cisplatin through modulations of GI enterochromaffin cells, serotonin and gastrin-producing cells and antioxidative systems. Especially HY showed the highest favorable effects more than those of ondansetron.

Abstract

Objectives This study aimed to observe the effect of Hyangsayangwi-tang on the cisplatin-induced gastrointestinal dysfunctions in rats. Methods Four groups(each of 8 rats per group) were used in this study. Saline and distilled water treated control rats are Intact vehicle control group. Delayed gatrointestinal mortility was induced by intraperitoneal treatment of cisplatin 2㎎/㎏, once a week for 5 weeks(Cisplatin control group). Hyangsayangwi-tang aqueous extracts(HY) were orally administered in a volume of 5㎖/㎏, once a day for 14 days from 4th ciplatin treatmernt(HY group). Ondansetron 1㎎/㎏ was subcutaneously treated, in a volume of 1㎖/㎏, as same as HY(ondansetron group). We measured the body weights, intestinal charcoal transit ratio, fecal parameters, fundus MDA, GSH contents and SOD, CAT activities, TPH and MAO activities, pyloric gastrin and serotonin contents with their immunoraective cells, colonic serotonin-immunoreactive cells, the histopathology of pylorus, fundus mucosa and colon. Results and Conclusions (1) The body weight gains, the small intestinal charcoal transfer rates, the fecal parameters(numbers, weights and water contents) were increased in HY, ondansetron group. (2) The inhibit of fundus antioxidant defense systems by cisplatin were decreased in HY, ondansetron group. (3) The pyloric TPH activities were increased and the pyloric MAO activities were decreased in HY group. (4) The pyloric gastric contents and the gastrin-immunoreactive cells were increased in HY group. And the pyloric serotonin contents and the pyloric and colonic serotonin-immunoreactive cells were decreased in HY group. (5) The pyloru atrophic changes and the gastric surface erosive damage regions by cisplatin were favorably inhibited by treatment of HY.HY, a representative Soeumin prescription improve GI dysfunctions and constipation retarded by cisplatin through modulations of GI enterochromaffin cells, serotonin and gastrin-producing cells and antioxidative systems. Especially HY showed the highest favorable effects more than those of ondansetron.

참고문헌 (41)

  1. Kishibayashi N, "Pharmacological properties of KF18259, a novel 5-HT3-receptor antagonist, inrats:inhibition of the distal colonic function", Jpn J Pharmacol, 63(4), pp.495~502 (1993)
  2. Lebwohl D, "Clinical development of platinum complexes in cancer therapy: an historical perspective and an update", Eur J Cancer, 34(10), pp.1522~1534 (1998)
  3. Andrews PL, "Signals for nausea and emesis: Implications for models of upper gastrointestinal diseases", Auton Neurosci, 125(1-2), pp.100~115 (2006)
  4. Martin C, "Anti-emetic effect of ondansetron and granisetron after exposure to mixed neutron and gamma irradiation", Radiat Res, 149(6), pp.631~636 (1998)
  5. Hafermann MJ, "Effect of intravenous ondansetron on QT interval prolongation in patients with cardiovascular disease and additional risk factors for torsades: a prospective, observational study", Drug Healthc Patient Saf, 3, pp.53~58 (2011)
  6. 김진철, "苦參이 抗癌劑 cisplatin의 肝?腎臟 副作用 減少에 미치는 影響", Korean Journal of Acupuncture, 22(3), pp.165~174 (2005), 경락경혈학회
  7. 남주영, "半枝蓮과 항암제 병용 투여에 의한 암세포 성장 저해에 관한 연구", 대한한방부인과학회지, 22(1), pp.125~139 (2009), 대한한방부인과학회
  8. 김현경, "시스플라틴과 녹차 추출물의 병용에 의한 세포성장 저해 효과", Journal of Gynecologic Oncology, 17(4), pp.347~353 (2006), 대한부인종양학회
  9. Rho YS, "Preventive Effect of Crude Drug Preparation (E-kong-san) on Cisplatin induced Nephrotoxicity", Kor. J. Pharmacogn, 29(3), pp.258~264 (1998)
  10. 조수인, "Beneficial Effect of Nodus Nelumbinis Rhizomatis Extract on Cisplatin-Induced Kidney Toxicity n Rats", 대한본초학회지, 18(4), pp.16~134 (2003), 대한본초학회
  11. 안창범, "黃芩藥鍼液이 Cisplatin에 의해 유발된 急性腎不全에 미치는 영향", 대한침구의학회지, 19(3), pp.156~167 (2002), 대한침구의학회
  12. 조시용, "車前子藥鍼이 急性腎不全 白鼠에 미치는 影響", Korean Journal of Acupuncture, 22(4), pp.117~127 (2005), 경락경혈학회
  13. Lee JM, "Donguisusebowon", pp.27~278 (2002), Eulyoo Publishing Co., Ltd
  14. Institute for Laboratory Animal Research, "Guide for the Care and Use of Laboratory Animals", pp.162~166 (1985), The national academics press
  15. Tyers MB, "5-HT3 receptors and the therapeuticpotential of 5-HT3 receptor antagonists", Therapie (Paris), 46(6), pp.431~435 (1991)
  16. Chirino YI, "Role of oxidative and nitrosative stress in cisplatin induced nephrotoxicity", Exp Toxicol Pathol, 61(3), pp.223~242 (2009)
  17. Chang B, "L-Carnitine inhibits cisplatin induced injury of the kidney and small intestine", Arch Biochem Biophys, 405(1), pp.55~64 (2002)
  18. SM Ahn, "Anti-Emetic Efficacy of Ondansetron Plus Desamethasone in Cisplatin- Based Combination Chemotherapy", Korean Journal of Obstetrics and Gynecology, 37(5), pp.976~994 (1994)
  19. Malik NM, "Differential effects of dexamethasone, ondansetron and a tachykinin NK1 receptor antagonist (GR205171) on cisplatin-induced changes in behaviour, food intake, pica and gastric function in rats", Eur J Pharmacol, 555(2-3), pp.164~173 (2007)
  20. Martin C, "Anti-emetic effect of ondansetron and granisetron after exposure to mixed neutron and gamma irradiation", Radiat Res, 149(6), pp.631~636 (1998)
  21. Badary OA, "In vitro and in vivo effects of ferulic acid on gastrointestinal motility: inhibition of cisplatin-induced delay in gastric emptying in rats", World J Gastroenterol, 12(33), pp.5363~5367 (2006)
  22. Kim YB, "A Randomized Comparison of Tropisetron versus Ondansetron in the Control of Nausea and Vomiting in Gynecologic Cancer Patients Undergone Cisplatin- Based Chemotherapy", Korean Journal of Obstetrics and Gynecology, 41(10), pp.2544~2550 (1998)
  23. National federation of department of sasang constitutional medicine, "Sasang constitutional medicine", pp.260~480 (2004), Jipmoondang
  24. 한경석, "少陰人 藥理의 形成過程 관한 硏究- 甲午本과 辛丑本을 중심으로 -", 사상체질의학회지, 18(2), pp.15~24 (2006), 사상체질의학회
  25. Kim KY, "The Theoretical Analysis on Soeumin-Hyangsayangwi-tang", J Sasang Constitut Med, 4(1), pp.213~219 (1992)
  26. Kim KY, "An Experimental Study on Some Effects of Soeumin-Hyangsayangwi-tang", J Sasang Constitut Med, 1(1), pp.153~170 (1989)
  27. 최병철, "香砂養胃湯 전탕액이 노화쥐의 비장, 췌장, 위장 세포의 항산화능에 미치는 영향", 사상체질의학회지, 20(2), pp.72~84 (2008), 사상체질의학회
  28. Wintola OA, "The effect of Aloeferox Mill. in the treatment of loperamide-induced constipationin Wistarrats", BMC Gastroenterol, 10, pp.95~ (2010)
  29. Sagar L, "Evaluation of antimotility effect of Lantanacamara L. var. acuelata constituents on neostigmine induced gastrointestinal transit in mice", BMC Complement Altern Med, 5, pp.18~ (2005)
  30. Yang ZH, "Cellular mechanisms underlying the laxative effect of flavonol naringenin on rat constipation model", PLoS One, 3(10), pp.e3348~ (2008)
  31. Comporti M., "Lipid peroxidation and cellular damage in toxic liver injury", Lab Invest, 53(6), pp.599~623 (1985)
  32. Odabasoglu F, "Gastroprotective and antioxidant effects of usnic acid on indomethacin-induced gastric ulcer in rats", J Ethnopharmacol, 103(1), pp.59~65 (2006)
  33. Cheeseman KH, "An introduction to free radical biochemistry", Br Med Bull, 49(3), pp.481~493 (1993)
  34. Süleyman H, "Comparative study on the gastroprotective potential of some antidepressants in indomethacininduced ulcer in rats", Chem Biol Interact, 180(2), pp.318~324 (2009)
  35. Lee HS, "Regional distribution and relative frequency of gastrointestinal endocrine cells in the ddN mice: an immunohistochemical study", Anat Histol Embryol, 39(6), pp.521~528 (2010)
  36. Bradner WT, "Toxicology screening in small animals", Cancer Treat Rev, 8(2), pp.93~102 (1981)
  37. Wang Y, "Role of vitamin d on the inhibition of gastrin production after Cisplatin treatment", Met Based Drugs, 7(3), pp.115~119 (2000)
  38. Gershon MD, "Release of endogenous 5-hydroxytryptamine from resting and stimulated enteric neurons", Neuroscience, 6(11), pp.2277~2286 (1981)
  39. Lee GN, "File of Clinical Pathology", pp.526~527 (1993), Medical Culture
  40. Huh So, "A 6.1 kb 5' upstream region of the mouse tryptophan hydroxylase gene directs expression of E, coli lacZ to major serotonergic brain region and pineal pland in transgenic mice", Brain Res Mol Brain Res, 24(1-4), pp.145~152 (1994)
  41. Lee JC, "New Sasang Constitutional Medicine 2", pp.79~249 (2003), Wood and Earth

관련 논문AI추천

이 간행물의 다른 논문 (15건)