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二陳湯이 정상 및 위 유문부가 부분폐색된 흰 쥐의 위 운동성에 미치는 효과

Effect of Yijin-tang on Gastric Motility Between Normal Intact and Partial Pyloric Obstructed Rats

윤상협|한숙영
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2009.03|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제30권 제1호|pp. 107-118

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키워드

Yijin-tangratgastric emptyingpartial pyloric obstructiongastric myoelectrical activity
주제분류한의학

초록

Objectives : This study was aimed to investigated the effect of Yijin-tang on gastric motility and its mechanism of action in normal intact and partial pyloric obstructed rats. Methods : Gastric emptying was measured by the number of glass beads expelled from the stomach (containing one hundred of glass beads, ∅1㎜) in 1 hour or 2 hours after glass beads and test drugs(normal saline, Yijin-tang 90㎎/㎏, Yijin-tang 270㎎/㎏) administration in normal intact and partial pyloric obstructed rats. In another series of experiments to evaluate the mechanism of Yijin-tang 270㎎/㎏ under delayed conditions, normal intact rats were treated with atropine sulfate(1㎎/㎏,s.c.), cisplatin(10㎎/㎏,i.p.), quinpirole HCl(0.3mg/kg,i.p.)and NAME(NG-nitro-L-arginine methyl ester, 75㎎/㎏,s.c.) respectively. Partial pyloric obstructed rats were performed by wrapping the nonabsorbable rubber ring(D:6mm, W:4mm, T:1mm) around 1st portion of duodenum for 8 weeks. The myoelectrical activity of the gastric smooth muscle was recorded by a bipolar electrode placed at the abdominal surface in normal intact and partial pyloric obstructed rats. The gastric myoelectrical activity was measured for 30 minutes before and after orogastric administration of each solution(normal saline, Yijin-tang 270㎎/㎏) and expressed as dominant frequency, percent of normogastria and power ratio. Results : Yijin-tang improved gastric emptying more than normal saline in normal intact(p<0.001) and partial pyloric obstructed rats(p=0.002). Under the delayed gastric emptying induced by atropine sulfate, cisplatin, quinpirole HCl and NAME, Yijin-tang enhanced gastric emptying significantly in the cisplatin treated group(p<0.001), but didn't in other treated groups. Administration of Yijin-tang 270㎎/㎏ has no significance effect on the myoelectrical activity of the gastric smooth muscle in both normal intact rats and partial pyioric obstructed rats. Conclusions : Yijin-tang seems to stimulate the gastric motility through suppressing the 5HT3 receptor and promoting the antroduodenal flow. We expect that Yijin-tang would be effective especially in dysmotility-like functional dyspepsia with partial pyloric obstruction or the side effects of cisplatin such as nausea, vomiting, abdominal discomfort, and delay of gastric emptying.

Abstract

Objectives : This study was aimed to investigated the effect of Yijin-tang on gastric motility and its mechanism of action in normal intact and partial pyloric obstructed rats. Methods : Gastric emptying was measured by the number of glass beads expelled from the stomach (containing one hundred of glass beads, ∅1㎜) in 1 hour or 2 hours after glass beads and test drugs(normal saline, Yijin-tang 90㎎/㎏, Yijin-tang 270㎎/㎏) administration in normal intact and partial pyloric obstructed rats. In another series of experiments to evaluate the mechanism of Yijin-tang 270㎎/㎏ under delayed conditions, normal intact rats were treated with atropine sulfate(1㎎/㎏,s.c.), cisplatin(10㎎/㎏,i.p.), quinpirole HCl(0.3mg/kg,i.p.)and NAME(NG-nitro-L-arginine methyl ester, 75㎎/㎏,s.c.) respectively. Partial pyloric obstructed rats were performed by wrapping the nonabsorbable rubber ring(D:6mm, W:4mm, T:1mm) around 1st portion of duodenum for 8 weeks. The myoelectrical activity of the gastric smooth muscle was recorded by a bipolar electrode placed at the abdominal surface in normal intact and partial pyloric obstructed rats. The gastric myoelectrical activity was measured for 30 minutes before and after orogastric administration of each solution(normal saline, Yijin-tang 270㎎/㎏) and expressed as dominant frequency, percent of normogastria and power ratio. Results : Yijin-tang improved gastric emptying more than normal saline in normal intact(p<0.001) and partial pyloric obstructed rats(p=0.002). Under the delayed gastric emptying induced by atropine sulfate, cisplatin, quinpirole HCl and NAME, Yijin-tang enhanced gastric emptying significantly in the cisplatin treated group(p<0.001), but didn't in other treated groups. Administration of Yijin-tang 270㎎/㎏ has no significance effect on the myoelectrical activity of the gastric smooth muscle in both normal intact rats and partial pyioric obstructed rats. Conclusions : Yijin-tang seems to stimulate the gastric motility through suppressing the 5HT3 receptor and promoting the antroduodenal flow. We expect that Yijin-tang would be effective especially in dysmotility-like functional dyspepsia with partial pyloric obstruction or the side effects of cisplatin such as nausea, vomiting, abdominal discomfort, and delay of gastric emptying.

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