少陰人 十二味寬中湯, 吳茱萸附子理中湯이 흰쥐의 腦와 肝組織의 抗酸化 機轉에 미치는 影響

The free radical theory of aging was introduced in 1956 by Denham Harman. This aging theory proposed that normal aging results from random deleterious damage to tissues by free radical and supplying antioxidant lead to decrease oxidative damage, inhibit aging process. In this study, we investigated antioxidantive effects of four Korean constitutional prescriptions for 'Soum' constitution - Palmulgunjatang(Y1), Sipyimiguanjungtang(Y2), Osuyubujayijungtang(Y3) and Seungyangyikkibujatang(Y4). Antioxidative activity of this prescriptions was examined by 1,1-diphenyl-2-picrylhyrdazyl radicals, superoxide anion radicals, peroxyl radical, hydroxyl radical scavenging effects and erythrocyte hemolysis inhibitory effects. Y2 and Y3 were shown to have relatively high antioxidative activity on this methods. In additions, result of the cytoprotective effects of Korean constitutional prescriptions agianst 2,2'-azobis(amidinopropane) dihydrochloride (AAPH), a free radical initiator, induced cytotoxcity in human hepatoblastoma cell line was similarly obtained. On the basis of this result, we assayed the antioxidative effects of Y2 and Y3 on experimental oxidative damage, induced in mouse by 100mg/kg AAPH. Male ICR mouse were given oral administration of 500mg/kg Y2 and Y3 for 4 weeks. Thiobarbuturic acid reactive substance (TBARS) and protein degradation level in liver, plasma and brain as index of oxidative damage were decreased and thiol compound, total antioxidant status in plasma were increased by Y2 administration. But, Y3 injected group was decreased only protein degradation level in brain. Also, glutathione, a potent water-soluble endogenous antioxidant, concentration was increased by Y2 and Y3 administration in liver and brain. However, superoxide dismutase and catalase activity as a major antioxidative enzyme in vivo were not shown change by Y2 and Y3 administration. On the basis of these result, Y2 have an antioxidative effects on both water-soluble fraction and lipid-solube fraction in cell and tissues. But, Y3 has a lower antioxidative effects on lipid-soluble fraction than Y2 in cell and tissues. These results suggest that Y2 has a antioxidative effects by protect the tissue against oxygen free radical mediated oxidative damage and Y3 has a limited antioxidaitve effects on water-soluble fraction in vivo. Therefore, we make report that Y2 is more effective prescriptions for anti-aging or therapeutics of diseases. 少陰人 十二味寬中湯과 吳茱萸附子理中湯의 抗酸化 效能을 檢證하기 위해, 藥材의 抗酸化力을 測定하였으며, 흰쥐에 投與하여 腦와 肝 組織의 抗酸化力 增强 效果를 實驗한 결과 有意性 있는 成績을 얻었기에 報告하는 바이다. 十二味寬中湯과 吳茱萸附子理中湯의 化學的 抗酸化力을 檢證한 結果 비교적 높은 抗酸化 活性을 보여주었다. HepG2 細胞株와 赤血球를 이용한 抗酸化力 檢證에서도 높은 活性을 보여주었으며. 특히 十二味寬中湯은 강한 수용성 抗酸化劑로 알려진 vitamin C와 유사한 결과를 보였다. 實驗用 흰쥐에게 湯劑 抽出物을 4週間 投與한 뒤 體重增加量과 GOT 活性을 測定한 결과 湯劑 非投與群과 有意的 差異를 보이지 않아 湯劑 投與에 의한 毒性은 없는 것으로 思料된다. 活性酸素 發生劑인 2.2'-azobis(amidinopropane) dihydrochloride (AAPH)로 酸化的 損傷을 誘發한 實驗用 흰쥐에서 十二味寬中湯 投與群은 血漿 總抗酸化力, 血漿 脂質過酸化가 抑制되었으며, 血漿 thiol 그룹의 減少도 抑制되었으나. 吳茱萸附子理中湯 投與群에서는 有意的 差異가 나타나지 않았다. 十二味寬中湯 投與群은 肝 및 腦組織에서 AAPH로 誘導된 酸化的 損傷을 有意的으로 抑制하였으며, 體內 重要 抗酸化 物質인 glutathione을 保護하여 體內 抗酸化 能力을 增進시킨 것으로 思料된다. 吳茱萸附子理中湯 投與群은 制限的인 效果만을 보였다. 十二味寬中湯은 抗酸化 效果가 높은 處方으로 볼 수 있는 바 臨床에서 老化防止에 널리 活用될 수 있을 것으로 思料된다.