치성상피가 골수줄기세포의 유전자 발현에 미치는 영향

Abstract

The dental epithelium produces the first inductive sign외s for od이ltogenesis such as FGF8, BMP4 and other unidentified factors These signals induce the expression of homeobox genes such as Lhx6, Lhx7, Pax9 and Msx1 which trigger off a cascade of events that res비t in the formation of toα，h. The aim of this study was identified the basic possibility of biotooth formation through interaction of odontogenic epithelium and bone marrow stem cell instead of ectomesenchymal cell on the basis of these tissue interaction in 않rly tooth development Bone marrow stem cell extracted from ICR mouse 없ld odontogenic epithelium and skin epithelium isolated from homologous embryo 암e recombinated by tissue recombination technique. Induction of early development marker such as Lhx6, Lhx7, Msx1, Pax9 and Axin2 by recombinated population is ar때yzed by RT-PCR All the genes (Lhx6, Lhx7, Pax9, Msx1, and Axin2l used in our experiment were expressed by recombinated population of dental epithelium and bone marrow stem cell. In recombinated population of skin epithelium and bone marrow stem cell, the expression of genes except for Lhx6 was identified. In cultured bone marrow stem cell, Pax9 and Msx1 were expressed. These results suggest that bone marrow stem cell can be used to rutificial tooth formation. Also as for the role of epithelial factor in the tooth formation, understanding of precise interaction of epithelium and stem cell will be important in the success of biotooth formation