생쥐 두개안면 형성과정에서 Npr3의 발현양상
Expression of Npr3 during the craniofacial morphogenesis of mouse
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Natriuretic peptides play physiological roles via their specific receptors, natriuretic peptide receptor-A, -B and -c. Unlike NPR-A and NPR-B, NPR-C does not mediate the intracellular signalling processes and called as silence or clearance receptor. A study with knockout mouse, NPR-C mediate the developmental processes of skeletal tissues. However, it is little known that NPR-C mediated signaling mechanisrns in the craniofacial development. Therefore, we purpcsed to analyse expression pattems of 1'1φr3 encoding NPR-C during craniofacial rnorphogenesis of mouse. At EIO.5, 1'1φr3 transcripts were specifically expressed in dorsal root ganglia, trigeminal ganglia, heart and meningeal 따tery. In the lower jaw with tongue of E12.5, Npr3 was restrictedIy expressed in the dorsum of tongue and incisor buds. In the rnaxilla of E13.5 and E14.5, expression of Npr3 was specifically observed in the intermaxillay segment extended to dorsum of the nose. In the developing tooth germs, 1\φr3 was expressed in the epithelial components of tooth germ from E13.5 to E16.5, corresponding to bud to bell stages of tooth development. In addition, expression of Npr3 were restricted in conjunctival and olfactory epithelium, trigeminal ganglion and salivary gland primordia. In conclusion, l\φr3 was specifically expressed in the neuronal structures, intermaxillary segment, intramembranous bone forming regions, tongue and conjunctival epitheliurn. Therefore, it is suggested that NPR-C may mediate the morphogenesis of craniofacial structures via altemative signaling processes.
Abstract
Natriuretic peptides play physiological roles via their specific receptors, natriuretic peptide receptor-A, -B and -c. Unlike NPR-A and NPR-B, NPR-C does not mediate the intracellular signalling processes and called as silence or clearance receptor. A study with knockout mouse, NPR-C mediate the developmental processes of skeletal tissues. However, it is little known that NPR-C mediated signaling mechanisrns in the craniofacial development. Therefore, we purpcsed to analyse expression pattems of 1'1φr3 encoding NPR-C during craniofacial rnorphogenesis of mouse. At EIO.5, 1'1φr3 transcripts were specifically expressed in dorsal root ganglia, trigeminal ganglia, heart and meningeal 따tery. In the lower jaw with tongue of E12.5, Npr3 was restrictedIy expressed in the dorsum of tongue and incisor buds. In the rnaxilla of E13.5 and E14.5, expression of Npr3 was specifically observed in the intermaxillay segment extended to dorsum of the nose. In the developing tooth germs, 1\φr3 was expressed in the epithelial components of tooth germ from E13.5 to E16.5, corresponding to bud to bell stages of tooth development. In addition, expression of Npr3 were restricted in conjunctival and olfactory epithelium, trigeminal ganglion and salivary gland primordia. In conclusion, l\φr3 was specifically expressed in the neuronal structures, intermaxillary segment, intramembranous bone forming regions, tongue and conjunctival epitheliurn. Therefore, it is suggested that NPR-C may mediate the morphogenesis of craniofacial structures via altemative signaling processes.