Regulation of odontoblast differentiation by Cpne7, a dental epithelium-derived factor, via the Runx2–Nfic–Osx–Dspp pathway
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Tooth development involves complex epithelial–mesenchymal interactions. In previous studies, disruption of these interactions was observed in nuclear factor I-C (Nfic)-deficient mouse incisors. Cpne7, a preameloblast-derived factor that can induce the promoter activity of the odontogenic marker dentin sialophosphoprotein (DSPP), was identified in a recent study. However, its relationship with the Nfic-mediated odontogenic pathway is unknown. To confirm the odontogenic ability of epithelial factors, MDPC-23 odontoblastic cells were cultured in a preameloblast-conditioned medium (PA-CM) obtained from mouse apical bud cells. The effects of dental epithelium on odontoblast differentiation were compared by culturing WT and Nfic−/− mouse incisor and molar primary pulp cells. To investigate the role of Cpne7 in the odontogenic signaling pathway, MDPC-23 cells were grown in PA-CM with CPNE7-Ab, and human dental pulp cells (hDPCs) were treated with recombinant Cpne7 (rCPNE7). Then, the expression of odontoblast-related genes was analyzed. Histological analysis of Nfic−/− mouse incisors revealed strong expression of RUNX2 in abnormal hard tissues adjacent to the dental epithelium, whereas the expression of OSX was not detected. Increased levels of Runx2 and down-regulation of Osx and Dspp were detected in Nfic−/− mouse incisor pulp cells, whereas no significant change was observed in molar pulp cells. In MDPC-23 cells, inactivation of Cpne7 in PA-CM inhibited the stimulatory effect of PA-CM on Runx2 and Osx. rCPNE7 treatment of hDPCs produced elevated levels of Osx, Dmp1, and Dspp. These findings suggest that the dental epithelial factor Cpne7 controls odontoblast differentiation of dental mesenchymal cells by inducing the Runx2–Nfic–Osx–Dspp signaling pathway.
Abstract
Tooth development involves complex epithelial–mesenchymal interactions. In previous studies, disruption of these interactions was observed in nuclear factor I-C (Nfic)-deficient mouse incisors. Cpne7, a preameloblast-derived factor that can induce the promoter activity of the odontogenic marker dentin sialophosphoprotein (DSPP), was identified in a recent study. However, its relationship with the Nfic-mediated odontogenic pathway is unknown. To confirm the odontogenic ability of epithelial factors, MDPC-23 odontoblastic cells were cultured in a preameloblast-conditioned medium (PA-CM) obtained from mouse apical bud cells. The effects of dental epithelium on odontoblast differentiation were compared by culturing WT and Nfic−/− mouse incisor and molar primary pulp cells. To investigate the role of Cpne7 in the odontogenic signaling pathway, MDPC-23 cells were grown in PA-CM with CPNE7-Ab, and human dental pulp cells (hDPCs) were treated with recombinant Cpne7 (rCPNE7). Then, the expression of odontoblast-related genes was analyzed. Histological analysis of Nfic−/− mouse incisors revealed strong expression of RUNX2 in abnormal hard tissues adjacent to the dental epithelium, whereas the expression of OSX was not detected. Increased levels of Runx2 and down-regulation of Osx and Dspp were detected in Nfic−/− mouse incisor pulp cells, whereas no significant change was observed in molar pulp cells. In MDPC-23 cells, inactivation of Cpne7 in PA-CM inhibited the stimulatory effect of PA-CM on Runx2 and Osx. rCPNE7 treatment of hDPCs produced elevated levels of Osx, Dmp1, and Dspp. These findings suggest that the dental epithelial factor Cpne7 controls odontoblast differentiation of dental mesenchymal cells by inducing the Runx2–Nfic–Osx–Dspp signaling pathway.
참고문헌 (32)
- Thesleff I, "Cell-matrix interactions in tooth development", Int J Dev Biol, 33, pp.91~97 (1989)
- Marianna Bei, "Molecular genetics of tooth development", Current Opinion in Genetics & Development, 19(5), pp.504~510 (2009), Elsevier BV[DOI]
- Thesleff I, "Signalling networks regulating dental development", Mech Dev, 67, pp.111~123 (1997)
- Nanci A, "Ten Cate's oral histology : development, structure, and function" (2013), Elsevier
- Nanci A., "Ten Cate's Oral Histology: Development, Structure, and Function" (2013), Elsevier (Singapore) Pte Limited
- Ten Cate AR, "The role of epithelium in the development, structure and function of the tissues of tooth support", Oral Dis, 2, pp.55~62 (1996)
- Makiko Arakaki, "Role of Epithelial-Stem Cell Interactions during Dental Cell Differentiation", Journal of Biological Chemistry, 287(13), pp.10590~10601 (2012), American Society for Biochemistry & Molecular Biology (ASBMB)[DOI]
- Ali Z. Chaudhry, "Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development", Developmental Dynamics, 208(3), pp.313~325 (1997), Wiley
- Gronostajski RM, "Roles of the NFI", CTF gene family in transcription and development, 2000, pp.249, 31~45
- Steele-Perkins G, "Essential role for NFI-C", CTF transcription-replication factor in tooth root development, 2003, pp.23, 1075~1084
- Hyun-Jung Oh, "Zinc balance is critical for NFI-C mediated regulation of odontoblast differentiation", Journal of Cellular Biochemistry, 113(3), pp.877~887 (2012), Wiley[DOI]
- Dong-Seol Lee, "NFI-C Regulates Osteoblast Differentiation via Control of Osterix Expression", STEM CELLS, 32(9), pp.2467~2479 (2014), Wiley[DOI]
- Hua Zhang, "Essential Role of Osterix for Tooth Root but not Crown Dentin Formation", Journal of Bone and Mineral Research, 30(4), pp.742~746 (2015), Wiley[DOI]
- Joo-Cheol Park, "Nfic Gene Disruption Inhibits Differentiation of Odontoblasts Responsible for Root Formation and Results in Formation of Short and Abnormal Roots in Mice", Journal of Periodontology, 78(9), pp.1795~1802 (2007), Wiley[DOI]
- Xiaofeng Huang, "Smad4-Shh-Nfic Signaling Cascade-Mediated Epithelial-Mesenchymal Interaction is Crucial in Regulating Tooth Root Development", Journal of Bone and Mineral Research, pp.091105035637074~40 (2009), Wiley[DOI]
- Dong-Seol Lee, "Nuclear Factor I-C Is Essential for Odontogenic Cell Proliferation and Odontoblast Differentiation during Tooth Root Development", Journal of Biological Chemistry, 284(25), pp.17293~17303 (2009), American Society for Biochemistry & Molecular Biology (ASBMB)[DOI]
- Hyun-Jung Oh, "CPNE7, a preameloblast-derived factor, regulates odontoblastic differentiation of mesenchymal stem cells", Biomaterials, 37, pp.208~217 (2015), Elsevier BV[DOI]
- Couble ML, "Odontoblast differentiation of human dental pulp cells in explant cultures", Calcif Tissue Int, 66, pp.129~138 (2000)
- Ji-Hyun Lee, "Odontogenic differentiation of human dental pulp stem cells induced by preameloblast-derived factors", Biomaterials, 32(36), pp.9696~9706 (2011), Elsevier BV[DOI]
- Paul Trainor, "Nuclear factor one transcription factors: Divergent functions in developmental versus adult stem cell populations", Developmental Dynamics, 244(3), pp.227~238 (2015), Wiley[DOI]
- Xiang Chen, "Expression of Nfic during root formation in first mandibular molar of rat", Journal of Molecular Histology, 45(6), pp.619~626 (2014), Springer Science and Business Media LLC[DOI]
- Hye-Kyung Lee, "Nuclear Factor I-C (NFIC) Regulates Dentin Sialophosphoprotein (DSPP) and E-cadherin via Control of Krüppel-like Factor 4 (KLF4) During Dentinogenesis", Journal of Biological Chemistry, 289(41), pp.28225~28236 (2014), American Society for Biochemistry & Molecular Biology (ASBMB)[DOI]
- Tong Ming Liu, "Transcriptional Regulatory Cascades in Runx2-Dependent Bone Development", Tissue Engineering Part B: Reviews, 19(3), pp.254~263 (2013), Mary Ann Liebert Inc[DOI]
- Monica Prasad Gibson, "The Rescue of Dentin Matrix Protein 1 (DMP1)-deficient Tooth Defects by the Transgenic Expression of Dentin Sialophosphoprotein (DSPP) Indicates That DSPP Is a Downstream Effector Molecule of DMP1 in Dentinogenesis", Journal of Biological Chemistry, 288(10), pp.7204~7214 (2013), American Society for Biochemistry & Molecular Biology (ASBMB)[DOI]
- Harada H, "Localization of putative stem cells in dental epithelium and their association with Notch and FGF signaling", J Cell Biol, 147, pp.105~120 (1999)
- Harada H, "FGF10 maintains stem cell compartment in developing mouse incisors", Development, 129, pp.1533~1541 (2002)
- Jingyuan Li, "BMP-SHH Signaling Network Controls Epithelial Stem Cell Fate via Regulation of Its Niche in the Developing Tooth", Developmental Cell, 33(2), pp.125~135 (2015), Elsevier BV[DOI]
- D'Souza RN, "Cbfa1 is required for epithelial-mesenchymal interactions regulating tooth development in mice", Development, 126, pp.2911~2920 (1999)
- Thomas Åberg, "Runx2 mediates FGF signaling from epithelium to mesenchyme during tooth morphogenesis", Developmental Biology, 270(1), pp.76~93 (2004), Elsevier BV[DOI]
- Karen Sagomonyants, "Biphasic effects of FGF2 on odontoblast differentiation involve changes in the BMP and Wnt signaling pathways", Connective Tissue Research, 55(sup1), pp.53~56 (2014), Informa UK Limited[DOI]
- Hu Zhao, "Secretion of Shh by a Neurovascular Bundle Niche Supports Mesenchymal Stem Cell Homeostasis in the Adult Mouse Incisor", Cell Stem Cell, 14(2), pp.160~173 (2014), Elsevier BV[DOI]
- Michael J. Dunn, "Conditioned media from cell lines: A complementary model to clinical specimens for the discovery of disease-specific biomarkers", PROTEOMICS, 11(4), pp.794~804 (2011), Wiley[DOI]