Nuclear Factor I-C 결손생쥐에서 치아의 형태학적 변화와 Smad4의 발현
Morphology of Tooth and Smad4 Expression in NFI-C Deficient Mouse
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Over expression of TGF-β1 revealed the same phenotype as NFI-C deficient mouse. It has been reported that NFI-C deficient mice demonstrated abnormal odontoblast differentiation and aberrant dentin formation during root development. In the present study, in order to investigate the histological differences between wild type (WT) mouse and NFI-C deficient mouse, we compared morphological characteristics and smad4 expression between those mice. Hematoxyline-eosin (H-E) staining was used to investigate morphological changes and immunohistochemistry was also performed to observe the Smad4 expression pattern. In H-E staining, incisor of NFI-C deficient mouse showed an open area in the lingual root, irregular odontoblasts and osteodentin. Also, NFI-C deficient mouse showed short root and osteodentin in molar. In addition, Smad4 protein was strongly expressed in NFI-C deficient mouse compared with wild type. These findings suggest that NFI-C deficiency affects odontoblast differentiation and result in the formation of abnormal roots. Therefore, balancing between NFI-C and TGF-β signaling including Smad4 is important for the regulation of normal odontoblast differentiation and dentin formation.
Abstract
Over expression of TGF-β1 revealed the same phenotype as NFI-C deficient mouse. It has been reported that NFI-C deficient mice demonstrated abnormal odontoblast differentiation and aberrant dentin formation during root development. In the present study, in order to investigate the histological differences between wild type (WT) mouse and NFI-C deficient mouse, we compared morphological characteristics and smad4 expression between those mice. Hematoxyline-eosin (H-E) staining was used to investigate morphological changes and immunohistochemistry was also performed to observe the Smad4 expression pattern. In H-E staining, incisor of NFI-C deficient mouse showed an open area in the lingual root, irregular odontoblasts and osteodentin. Also, NFI-C deficient mouse showed short root and osteodentin in molar. In addition, Smad4 protein was strongly expressed in NFI-C deficient mouse compared with wild type. These findings suggest that NFI-C deficiency affects odontoblast differentiation and result in the formation of abnormal roots. Therefore, balancing between NFI-C and TGF-β signaling including Smad4 is important for the regulation of normal odontoblast differentiation and dentin formation.
참고문헌 (21)
- Mina M, "The induction of odontogenesis in nondental mesenchyme combined with early murine mandibular arch epithelium", Arch Oral Biol, 32, pp.123~127 (1987)
- Maas R, "The genetic control of early tooth development", Crit Rev Oral Biol Med, 8(1), pp.4~39 (1997)
- Ruch JV, "Odontoblast differenciation", Int J Dev Biol, 39(1), pp.51~68 (1995)
- Antonio Nanci, "Ten cate’s oral histology: Development, structure, and function. 7th ed", pp.89~103 (2007), MOSBY
- Garant PR, "The organization of microtubules within rat odontoblast processes revealed by perfusion fixation with glutaraldehyde", Arch Oral Biol, 17(7), pp.1047~1058 (1972)
- Bosshardt DD, "Hertwig's epithelial root sheath matrix proteins and initiation of cemetogenesis in porcine teeth", J Periodontal, 31(3), pp.184~192 (2004)
- Steele- parkins G, "Essental role NFI-C", CTF transcription _ replication factor in tooth root development, 2003, pp.23(3), 1075~1084
- Chaudhry AZ, "Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development", Dev Dye, 208(3), pp.313~325 (1997)
- Chaudhry AZ, "Nuclear factor I (NFI) isoforms differentially activate simple versus complex NFI-responsive promoters", J Biol Chem, 273(29), pp.18538~18546 (1998)
- Mukhopadhyay SS, "Differential interactions of specific nuclear factor I isoforms with the glucocorticoid receptor and STAT5 in the cooperative regulation of WAP gene transcription", Mol Cell Biol, 21(20), pp.5859~6869 (2001)
- Ling G, "Transcriptional regulation of rat CYP2A3 by nuclear factor 1: Identification of a novel NFI-A isoform, and evidence for tissue-selective interaction of NFI with the CYP2A3 promoter in vivo", J Biol Chem, 279(27), pp.27888~27895 (2004)
- Shu T, "Abnormal development of forebrain midline glia and commissural projections in Nfia knock-out mice", J Neurosci Jan, 23(1), pp.203~212 (2003)
- Park JC, "Nfic gene disruption inhibits differentiation of odontoblasts responsible for root formation and results in formation of short and abnormal roots in mice", J Periodontol, 78(9), pp.1795~1802 (2007)
- 배현숙, "상아모세포 분화과정에서 nuclear factor Ι-C의 역할", 대한구강악안면병리학회지, 29(2), pp.83~92 (2005), 대한구강악안면병리학회
- 배현숙, "Nuclear factor I-C 과발현과 발현억제가 MDPC-23 상아모세포주의 상아질 기질유전자 발현에 미치는 영향", 치위생과학회지, 9(4), pp.427~433 (2009), 한국치위생과학회
- Thyagarajan T, "Reduced expression of dentin sialophosphoprotein is associated with dysplastic dentin in mice over expressing transforming growth factor-beta 1 in teeth", J Biol Chem, 276(14), pp.11016~11020 (2001)
- 윤성호, "NFI-C가 Smad와 TGF-βR1의 발현에 미치는 영향", Anatomy and Cell Biology, 40(2), pp.127~135 (2007), 대한해부학회
- KwangYoulLee, "TGF-β-dependent Cell Growth Arrest and Apoptosis", BMB reports, 35(1), pp.47~53 (2002), 한국생화학분자생물학회
- Bruce Alberts, "Molecular biology of the cell. 5th ed", pp.393~940 (2008), Garland Science
- Yuanrong Gao, "Disruption of Smad4 in odontoblast causes multiple keratocystic odontogenic tumors and tooth malformation in mice", Mol Cell Biol, 29(21), pp.1942~1944 (2009)
- Aristidis M, "Smad regulation in TGF-β signal transduction", J Cell Sci, 114(24), pp.4363~4366 (2001)