A comparison of odontogenic gene expression in crown and root odontoblasts
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
With the new functional discovery of stem cells from apical papilla (SCAP), there has been identified difference between SCAP and dental pulp stem cells (DPSCs) in gene expression. SCAP and DPSC, which are both human mesenchymal stem cells extracted from two histologically similar but distinct tissues, have different potential regeneration abilities. For deeper understanding of distinct regeneration ability, the difference among SCAP, crown pulp cells, and root pulp cells must be studied. The purpose of this paper is to identify the main differences of gene expression among SCAP, crown pulp, and root pulp and to discover the possibility of origin difference of crown dentin and root dentin. In the Real time PCR gene expression chart, crown pulp showed higher expression level of Runx2 than that of SCAP and root pulp. In the western blot assay, both expression levels c-IAP and CDK2 showed similar pattern. The gene expression levels were higher in SCAP and root pulp cells than in crown pulp cells. In addition, c-IAP and CDK2 expression levels in SCAP and root pulp cells were similar. The results suggested that these comparison data show that the origin of the crown pulp could be different from the origin of the root pulp. Thus, there is a possibility that the crown dentin and root dentin have different origin.
Abstract
With the new functional discovery of stem cells from apical papilla (SCAP), there has been identified difference between SCAP and dental pulp stem cells (DPSCs) in gene expression. SCAP and DPSC, which are both human mesenchymal stem cells extracted from two histologically similar but distinct tissues, have different potential regeneration abilities. For deeper understanding of distinct regeneration ability, the difference among SCAP, crown pulp cells, and root pulp cells must be studied. The purpose of this paper is to identify the main differences of gene expression among SCAP, crown pulp, and root pulp and to discover the possibility of origin difference of crown dentin and root dentin. In the Real time PCR gene expression chart, crown pulp showed higher expression level of Runx2 than that of SCAP and root pulp. In the western blot assay, both expression levels c-IAP and CDK2 showed similar pattern. The gene expression levels were higher in SCAP and root pulp cells than in crown pulp cells. In addition, c-IAP and CDK2 expression levels in SCAP and root pulp cells were similar. The results suggested that these comparison data show that the origin of the crown pulp could be different from the origin of the root pulp. Thus, there is a possibility that the crown dentin and root dentin have different origin.
참고문헌 (17)
- Potdar PD, "Human dental pulp stem cells : Applications in future regenerative medicine", World Journal of Stem Cells, 7(5), pp.839~851 (2015)
- Gronthos S, "Stem cell properties of human dental pulp stem cells", J Dent Res, 81, pp.531~535 (2002)
- Sonoyama W, "Mesenchymal stem cell-mediated functional tooth regeneration in Swine", PLoS ONE, 1, pp.e79~ (2006)
- Huang G, "The hidden treasure in apical papilla: The potential role in pulp", dentin regeneration and BioRoot Engineering, 2008, pp.34, 645~651
- Mauth C, "Topics in Tissue Engineering", pp.1~30 (2007)
- Lee DS, "Nuclear factor i-c is essential for odontogenic cell proliferation and odontoblast differentiation during tooth root development", J Biol Chem, 284(25), pp.17293~17303 (2009)
- Lee DS, "Nfi-c regulates osteoblast differentiation via control of osterix expression", Stem Cells, 32(9), pp.2467~2479 (2014)
- Wu J, "Basic fibroblast growth factor enhances stemness of human stem cells from the apical papilla", J Endod, 38(5), pp.614~622 (2012)
- Shoi K, "Characterization of pulp and follicle stem cells from impacted supernumerary maxillary incisors", Pediatr Dent, 36, pp.79~84 (2014)
- Zhang W, "Multilineage differentiation potential of stem cells derived from human dental pulp after cryopreservation", Tissue Eng, 12, pp.2813~2823 (2006)
- Sonoyama W, "Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study", J Endod, 34, pp.166~171 (2008)
- Sato M, "Characterization of mesenchymal progenitor cells in crown and root pulp from human mesiodentes", Oral Dis, 21, pp.e86~e97 (2015)
- Seo BM, "Investigation of multipotent postnatal stem cells from human periodontal ligament", Lancet, 364, pp.149~155 (2004)
- Bakopoulou A, "Comparative analysis of in vitro osteo", odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP), 2011, pp.56(7), 709~721
- Ge Y, "The role of odontogenic genes and proteins in tooth epithelial cells and their niche cells during rat tooth root development", Arch Oral Biol, 58(2), pp.151~159 (2013)
- Wang J, "Effects of Wnt", β-catenin signalling on proliferation and differentiation of apical papilla stem cells, 2012, pp.45(2), 121~131
- Wang S, "Insulin-like growth factor 1 can promote the osteogenic differentiation and osteogenesis of stem cells from apical papilla", Stem Cell Res, 8(3), pp.346~356 (2012)