Combination of Aptamer-Functionalized Quantum Dots and Electrophoretic Mobility Shift Assay for Specific Detection and Quantitation of Proteins of Interest
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Quantum dots (QDs) are semiconducting nanoparticles with interesting optical properties. The outstanding fluorescent properties of QDs including photostability, high quantum yield, narrow emission bands, broad excitation spectra, and accessibility to versatile functionalization are one of the key advantages for their sensing applications. In the present study, we report QD-functionalized aptamers for specific detection and quantitation of proteins of interest, based on electrophoretic mobility shift assay (EMSA). Among the wide variety of potential biofunctional groups, we used the highly selective interaction between aptamers and oligohistidine or human chorionic gonadotropin (hCG), and the results demonstrate that the combination of the QD-functionalized aptamers and EMSA can be one of the best sensing options for protein quantitation with low limit of detection. In principle, multiplex detection for several proteins on a single gel can be achieved by our method, leading to facilitation and simplification of the routinely used protein detection procedure.
Abstract
Quantum dots (QDs) are semiconducting nanoparticles with interesting optical properties. The outstanding fluorescent properties of QDs including photostability, high quantum yield, narrow emission bands, broad excitation spectra, and accessibility to versatile functionalization are one of the key advantages for their sensing applications. In the present study, we report QD-functionalized aptamers for specific detection and quantitation of proteins of interest, based on electrophoretic mobility shift assay (EMSA). Among the wide variety of potential biofunctional groups, we used the highly selective interaction between aptamers and oligohistidine or human chorionic gonadotropin (hCG), and the results demonstrate that the combination of the QD-functionalized aptamers and EMSA can be one of the best sensing options for protein quantitation with low limit of detection. In principle, multiplex detection for several proteins on a single gel can be achieved by our method, leading to facilitation and simplification of the routinely used protein detection procedure.
참고문헌 (19)
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