Streptomyces sp. AMLK -1357가 생산하는 항 메치실린 내성균성 항생물질의 분리 정제 및 이화학적 성질 조사

Abstract

This study was carried out to isolate and purify anti-MRSA (methicillin-resistant Staphylococcus aureus) TK784 antibiotics produced by Streptomyces sp. AMLK-135 which isolated from soil. Consequently, characteristics of purified antibiotics were investigated. The results obtained were summarized as follows 1. The culture broth of Streptomνces sp. AMLK-135 possessed anti-MRSA activity against Staphylococcus aureus TK-784, and it showed anti-microbial activity against Bacillus subtilis IAM 1069, Staphylococcus aureus FDA 2089, Escherichia coli AB 1157, Pseudomonas jluorescence IAM 1201, Chlorella regularis and Chlorella sp. A2dl 2. By the separation and purification of anti-MRSA antibiotic from Streptomνces sp. AMLK-135 using amberlite lRA-400 ion exchange column chromatography, butanol extraction, cellulose column chromatography, sephadex LH-20 gel permeation column chromatography and HPLC (mobile phase: 35% methanol), the antibiotic AM-1 was obtained as a single peak with excellent resolution. 3. The anti-MRSA antibiotic AM-1 possessed a oily property, soluble in MeOH and water and had a maximum UV absorption of 230 nm4. The infrared spectrum of the antibiotic AM-1 showed absorption bands at 3500-3000cm-1 (hydroxyl, -OH), 2520 cm-1 (amine, N-H) and 1451 cm-1 (β-lactone, C=O, C-O).