임상검체에서 분리된 MRSA 검출법과 항생제 감수성검사의 성상

Abstract

Nosocomial infection due to methicillin resistance Staphylococcus aureus(MRSA) is increasing and poses a serious problem in our country, this study were conducted to assess the characteristics of susceptibility test and detection method of MRSA isolated from clinical specimens. 386 S. aureus strains isolated from Dong - A hospital from January, 1994 to December, 1994. Among 386 clinical isoletes of S. aureus which were tested, 180 strains( 46.6%) were oxacillin resistant, and 5 strains( 1.3%) showed intermediate results. Distribution of MRSA isolated from clinical specimens were pus(71.1%), respiratory(17.8%), blood(5.6%), urine(3.3%), fluid(2.2%). 180 strains methicillin resistant S. aureus isolates , of which 166 strains were definitive resistant and 14 strains were occult resistant, were examined. All definitive resistant strains were resistant to all cooler temperature( 30℃ ), prolonged incubation(48hr's) the presence of sodium chloride(NaCl 2 or 4%), gentamicin disk methods, but occult resistant strains were difference result. Definitive resistant isolates were characterized by a very high and stable frequency of resistant, occult resistant strains were chracterized by a lower frequency of resistance, althouth the true frequency of resistance may be difficult to ascertain because of heterogeneity in growth rates. Most of MRSA were simultaneously resistant to various antibiotics. Simultaneously resistant of ampicillin, clindamycin, penicillin, tetracycline were 24 %, which ampicillin, ciprofloxacin, clindamycin, erythromycin, penicillin, tetracycline was 56.1 %, resistant strains of over five antibiotics were 88.9 %. Particularly, vancomycin and teichoplamin showed absence of resistance. On the basis of these results , we can conclude that MRSA were quite prevalent at hospital and multiple resistant pattern against various antibiotics.