Virulence Genes of Staphylococcus aureus Isolated in Daegu and Gyeongsangbuk-do Areas
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Abstract
Nine types of staphylococcal enterotoxin (SE) genes (sea~see, seg~sej), 3 types of virulence genes (eta, etb, tst), mecA and 16S rRNA as internal positive control were detected from 187 clinical MRSA (methicillin resistance Staphylococcus aureus) strains isolated from a variety hospitalized patients in Daegu and Gyeongsangbuk-do areas using the multiplex PCR. The frequency of the S. aureus strains harboring recently reported SE genes (seg~sej) were found to be very high (65.9%) and greater than that of the strains harboring classical SE (sea~see) genes (47.8%) as previously established. Taking into account that the newly described pairs form SE genes (i.e., sec+seg+sei, seg+sei) were many, in the other hand, single form SE genes (i.e., seg, seh, sei and sej) were rarely detected. The S. aureus with pairs form enterotoxigenic genes become more potentially toxigenic strains. Furthermore, this work indicated a systematic association between the seg and sei genes and their high incidence among the S, aureus strains, which suggests that these two SE’s could be an important phylogenetic link among the staphylococcal enterotoxins.
참고문헌 (19)
- Altschul SF, "Basic local alignment search tool", J Mol Biol, 215, pp.403~410 (1990)
- Betley MJ, "Staphylococcal enterotoxin A is encoded by phage", Science, 229, pp.185~187 (1985)
- Bohach, GA, "Staphyloccocal and streptococcal pyrogenic toxins involved in toxic shock syndrome and related illnesses", Crit Rev Microbiol, 17, pp.251~272 (1990)
- Boyce JM, "The staphylococci in human disease", pp.309~329 (1997), Churchill Livingstone Publishing
- Holeckova B, "Occurrence of Enterotoxigenic Staphylococcus aureus in Food", Ann Agric Environ Med, 9, pp.179~182 (2002)
- Hookey JV, "Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of coagulase gene", J Clin Microbiol, 36, pp.1083~1089 (1998)
- Jackson MP, "Cloning and expression of the exfoliative toxin B gene from Staphylococcus aureus", J Bacteriology, 166, pp.574~580 (1986)
- Jarraud S. Cozon G, "Involvement of enterotoxins G and I in staphylococcal toxic shock syndrome and staphylococcal scarlet fever", J Clin Microbiol, 3, pp.2446~2449 (1999)
- John VH, "Molecular typing of Staphylococcus based on PCR restriction fragment length ploymophism and DNA sequence analysis of the coagulase gene", J Clin Microbiol, 36(4), pp.1083~1089 (1998)
- Lindsay JA, "Clinical isolates of Staphylococcus aureus encode TSST-1 on genetic elements related to S. aureus pathogenicity, island-1(SaPI1)", European Conference on Toxic Shock Syndrome (1997), Royal Society of Medicine
- Mac Lauchlin J, "The detection of enterotoxins and toxic shock syndrome toxin genes in Staphylococcus aureus by polymerase chain reaction", J Food Port, 63, pp.479~488 (2000)
- Munson SH, "Identification and characterization of staphylococcal enterotoxin types G and I from Staphylococcus aureus", Infect Immun, 66, pp.3337~3348 (1998)
- Projan, SJ, "The staphylococci in human disease", pp.5~81 (1997), Churchill Livingstone, Publishing
- Rosec JP, "Staphylococcal isolates de produits alimentaires : detection des souches enterotoxinogenes par PCR", Ann Falsif Expert Chim, 943, pp.157~173 (1998)
- Tornadijo ME, "Population levels, species and characteristics of Micrococcaceae during the manufacturing and preparing of Armada-sobado goat's milk cheese", J Food Prot, 59, pp.1200~ (1996)
- Vincent JL, "The prevalence of nosocomial infection in intensive care units in Europe. Result of the european prevalence of infection in intensive care (EPIC) study. EPIC International Advisory Committee", JAMA, pp.639~644 (1995)
- Yamaguchi TT, "Phage conversion of exfoliative toxin A production in Staphylococcus aureus", Mol Microbiol, 38, pp.694~705 (2000)
- Yoshizawa Y, "An exfoliative toxin A-converting phage isolated from Staphylococcus aureus strain ZM", Microbiol Immunol, 44, pp.189~191 (2000)
- Zhang S, "The enterotoxin D plasmid of Staphylococcus aureus encodes a second enterotoxin determinant(sej)", FEMS Microbiol Lett, 168, pp.227~233 (1998)