Distribution of Virulence Genes in spa Types of Methicillin-resistant Staphylococcus aureus Isolated from Patients in Intensive Care Units
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Background: Various virulence factors and superantigens are encoded by mobile genetic elements. The relationship between clonal background and virulence factors differs in different geographic regions. We compared the distribution and relationship of spa types and virulence genes among methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from a tertiary hospital in 2000-01 and 2007-08. Methods: In 2000-01 and 2007-08, 94 MRSA strains were collected from 3 intensive care units at a Korean tertiary hospital. We performed spa typing and multiplex PCR for 19 superantigen genes. Results: Relatively frequent spa types were t037 (40.5%), t002, t601, and t2138 in 2000-01, and t2460 (43.9%), t002, t037, t601, t324, and t2139 in 2007-08. We identified 4 novel spa types, 2 of which were designated as t5076 and t5079. Superantigen profiles were closely linked to spa types. For example, sea, sek, and seq superantigen genes were mainly detected in t037 strains. Conclusions: Major spa types differed depending on study periods, and the distribution of superantigen genes correlated with spa type.
Abstract
Background: Various virulence factors and superantigens are encoded by mobile genetic elements. The relationship between clonal background and virulence factors differs in different geographic regions. We compared the distribution and relationship of spa types and virulence genes among methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from a tertiary hospital in 2000-01 and 2007-08. Methods: In 2000-01 and 2007-08, 94 MRSA strains were collected from 3 intensive care units at a Korean tertiary hospital. We performed spa typing and multiplex PCR for 19 superantigen genes. Results: Relatively frequent spa types were t037 (40.5%), t002, t601, and t2138 in 2000-01, and t2460 (43.9%), t002, t037, t601, t324, and t2139 in 2007-08. We identified 4 novel spa types, 2 of which were designated as t5076 and t5079. Superantigen profiles were closely linked to spa types. For example, sea, sek, and seq superantigen genes were mainly detected in t037 strains. Conclusions: Major spa types differed depending on study periods, and the distribution of superantigen genes correlated with spa type.
참고문헌 (16)
- Holtfreter S, "Clonal distribution of superantigen genes in clinical Staphylococcus aureus isolates", J Clin Microbiol, 45, pp.2669~2680 (2007)
- Diep BA, "Roles of 34 virulence genes in the evolution of hospital- and community-associated strains of methicillin-resistant Staphylococcus aureus", J Infect Dis, 193, pp.1495~1503 (2006)
- Niemeyer DM, "Role of mecA transcriptional regulation in the phenotypic expression of methicillin resistance in Staphylococcus aureus", J Bacteriol, 178, pp.5464~5471 (1996)
- Koreen L, "spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro- and macrovariation", J Clin Microbiol, 42, pp.792~799 (2004)
- Frénay HM, "Molecular typing of methicillin- resistant Staphylococcus aureus on the basis of protein A gene polymorphism", Eur J Clin Microbiol Infect Dis, 15, pp.60~64 (1996)
- Lindsay JA, "Microarrays reveal that each of the ten dominant lineages of Staphylococcus aureus has a unique combination of surface- associated and regulatory genes", J Bacteriol, 188, pp.669~676 (2006)
- National Committee for Clinical Laboratory Standards, "Performance standards for antimicrobial susceptibility testing" (2002), National Committee for Clinical Laboratory Standards
- Clinical and Laboratory Standards Institute, "Performance standards for antimicrobial susceptibility testing" (2009), Clinical and Laboratory Standards Institute
- Mehrotra M, "Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance", J Clin Microbiol, 38, pp.1032~1035 (2000)
- Hwang SY, "Novel multiplex PCR for the detection of the Staphylococcus aureus superantigen and its application to raw meat isolates in Korea", INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 117, pp.99~105 (2007), ELSEVIER SCIENCE BV
- Aires de Sousa M, "Comparison of genetic backgrounds of methicillin-resistant and -susceptible Staphylococcus aureus isolates from Portuguese hospitals and the community", J Clin Microbiol, 43, pp.5150~5157 (2005)
- Witte W, "Methicillin-resistant Staphylococcus aureus containing the Panton-Valentine leucocidin gene in Germany in 2005 and 2006", J Antimicrob Chemother, 60, pp.1258~1263 (2007)
- Varshney AK, "Diverse enterotoxin gene profiles among clonal complexes of Staphylococcus aureus isolates from the Bronx, New York", Appl Environ Microbiol, 75, pp.6839~6849 (2009)
- Jarraud S, "egc, a highly prevalent operon of enterotoxin gene, forms a putative nursery of superantigens in Staphylococcus aureus", J Immunol, 166, pp.669~677 (2001)
- Hu DL, "Comparative prevalence of superantigenic toxin genes in meticillin- resistant and meticillin-susceptible Staphylococcus aureus isolates", J Med Microbiol, 57, pp.1106~1112 (2008)
- 백경란, "Comparison of Genotypes and Enterotoxin Genes Between Staphylococcus aureus Isolates from Blood and Nasal Colonizers in a Korean Hospital", Journal of Korean Medical Science, 24(4), pp.585~591 (2009), 대한의학회