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사염화탄소, Dimethylnitrosamine, Thioacetamide의 3가지 시약과 쥐에게 간섬유증을 유도하는데 이용되는 담관결찰로 인한 조직병리학적 변화의 비교

Comparison of Histopathological Changes on the Three Drugs of Carbon Tetrachloride, Dimethylnitrosamine, Thioacetamide, and Bile Duct Ligation used for Induction of Liver Fibrosis in Rat

김진영|박미정|김정훈|박수영|신진희|제갈승주
대한임상검사과학회 (Korean Society for Clinical Laboratory Science)|2011.12|대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)|제43권 제4호|pp. 194-204

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Abstract

This study was carried out to compare the histopathological differences of liver lesions in carbon tetrachloride (CCI4), dimethylnitrosamine (DMN), thioacetamide (TAA) and bile duct ligation (BDL)-induced rats. CCl4, DMN and TAA were administered intraperitoneally and conducted bile duct ligation for 4 weeks to induce hepatic fibrosis. Indices of liver cell injury (steatosis, hydropic degeneration, bile duct hyperplasia, hemorrhage & hemosiderin deposition), the extent of liver fibrosis (fibrotic area) and the rate of regeneration (number of PCNA-positive cells) were investigated in each group. Liver tissues were stained with hematoxylin-eosin (HE), sirius red, prussian blue and immunostained with α-smooth muscle actin (α-SMA), transforming growth factor-β1 (TGF-β1), proliferative cell nuclear antigen (PCNA), and quantified using a computerized image analysis system. Liver cell steatosis was significantly increased in CCl4 and TAA groups, and hydropic degeneration and bile duct hyperplasia were significantly increased in TAA and BDL groups when compared with that in normal control, respectively. Fibrosis area was significantly increased in all four groups, especially in CCl4 group. Correlation between α-SMA and TGF-β1 expressions in four groups was good. Hemorrhage area in liver parenchyma was significantly increased in DMN group only when compared with that in normal control, while hemosiderin deposition area was significantly increased in TAA and BDL groups as well as DMN group. The Number of PCNA-positive cells was significantly increased in all four groups, especially in TAA group. These results indicate that the duration and methods of hepatotoxic drug treatment are very important factors to make plans for animal experimentation on the induction of hepatic fibrogenesis in rats.

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사염화탄소와 Dimethylnitrosamine의 반복투여가 백서간의 형태학적 변화에 미치는 영향이태숙영남대학교 의과대학 · Journal of Yeungnam Medical Science1987 · 제4권 제1호사염화탄소 유도 간섬유증 발생에 있어서 비만세포와 근섬유모세포의 상호 관련성제갈승주대한임상검사과학회 · 대한임상검사과학회지2001 · 제33권 제2호실험적 흰쥐 간 질환 모델의 이해 -형태학적 특징을 중심으로-박도윤, 서강석대한병리학회 · Journal of Pathology and Translational Medicine2003 · 제37권 제3호사염화탄소 투여가 Dimenthylnitrosamine의 급성중독 간세포의 구조에 미치는 영향강영천, 김동석, 남혜주, 이태숙, 최원희영남대학교 의과대학 · Journal of Yeungnam Medical Science1991 · 제8권 제2호사염화탄소의 長期 投與로 유발된 白鼠 肝硬變에 대한 荊防地黃湯의 조직병리학적 영향김승모대한한방내과학회 · 대한한방내과학회지2008 · 제29권 제2호DMN으로 유발된 흰쥐의 간섬유화에 미치는 대계의 효과길재호, 박성규, 배현수, 신민규, 이은주, 홍무창한의병리학회 · 동의생리병리학회지2004 · 제18권 제2호

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