사염화탄소 유도 간섬유증 발생에 있어서 비만세포와 근섬유모세포의 상호 관련성

Abstract

m2 was determined by counting mast cells using a computerized image analyser system in liver sections stained with toluidine blue and alcian blue-safranin O, respectively. Also, The morphological relation between mast cells and myofibloblasts was examined by elecπon microscopy. The collagen content was significantly increased at 4 to 6week after CCl4 treatment compared with control rats. These results showed significant correlations (Spearman's, r=0.76, p<0.001) with those of a numerical scoring system. In control rats,mast cells were found principally in portal areas, and their average density was 4.76±2.23/㎟ in liver sections stained with alcian blue-safranin O. Whereas in CCl4-treated rats mast cells were diffusely distributed in fibrous septa and fibrous capsule on liver surface and were significantly increased at 2(8.86±1.94/㎟), 4(12.26±3.59/㎟) to 6week(28.09±12.7/㎟) after CCl4 treatment compared with control rats. In cαltrol rats, myofibroblasts were not found in portal areas and observed only a-SMA positive staining on vessel walls in portal areas. In CCl4-treated rats, myofibroblasts were occasionally found at 2week after CCl4 treatment in portal areas, fibrous septa and liver parencyma, but were statistically not significant compared with control rats. Myofibrobasts were significantly increased at 4 to 6week after CCl4 treatment. Ultrastructurally, mast cells were observed in close contact with myofibroblasts in fibrous septa and showed the various stages of degranulation. These results suggested that mast cell may play role in development of hepatic fibrosis, probably because of the production of extracellular maπix components by myofibroblasts which activate bysome mediators released from mast cells.