세포원심분리기를 사용한 뇌척수액산정검사의 세포회수율
Cell Yield of Cerebrospinal Fluid Cell Count Using Cytocentrifuges
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The cells are concentrated approximately 20-fold by cytocentrifugation. This study evaluated the nucleated cell number for cells recovered on slide by using Cytospin-3 (Thermo Shandon Ltd. UK) and Cytopro-7620 (Wescor Inc., USA) cytocentrifuges to hematocytometer cell count of 0∼5 WBCs/μL of hematocytometer in the cerebrospinal fluid cell count. One hundred forty eight samples of 0∼5 WBCs/μL on hematocytometer, were cytocentrifuged by Cytospin-3 and Cytopro-7620 instruments. The nucleated cell number for cells recovered on slide was counted after Wright stain. The nucleated cell number for cells recovered on slide was 0∼40 cells in the 44 samples of 0 WBC/μL, and 3∼95 cells in the 31 samples of 1 WBC/μL. It was observed that the nucleated cell number for cells recovered on slide was 13∼100 cells in the 44 samples of 2 WBCs/μL, and more than 100 cells in the 29 samples of 3∼5 WBCs/μL, respectively. In addition, extremely normal lymphocyte, monocyte and polymorphonuclear neutrophil were observed in the 143 samples of 0∼5 WBCs/μL. Macrophage and eosinophil were also rarely observed. The nucleated cell number for cells recovered on slide was 20 cells, which were regarded as 1 WBC/μL in body fluid cell count. However, in this study, we made alterations to report nucleated cell percentage as 0% without preparing the cytocentrifuged slide at 0 WBC/μL by using the cell yield in a comparison between the value of 0∼5 WBCs/μL and nucleated cell number for cells recovered on slide.
Abstract
The cells are concentrated approximately 20-fold by cytocentrifugation. This study evaluated the nucleated cell number for cells recovered on slide by using Cytospin-3 (Thermo Shandon Ltd. UK) and Cytopro-7620 (Wescor Inc., USA) cytocentrifuges to hematocytometer cell count of 0∼5 WBCs/μL of hematocytometer in the cerebrospinal fluid cell count. One hundred forty eight samples of 0∼5 WBCs/μL on hematocytometer, were cytocentrifuged by Cytospin-3 and Cytopro-7620 instruments. The nucleated cell number for cells recovered on slide was counted after Wright stain. The nucleated cell number for cells recovered on slide was 0∼40 cells in the 44 samples of 0 WBC/μL, and 3∼95 cells in the 31 samples of 1 WBC/μL. It was observed that the nucleated cell number for cells recovered on slide was 13∼100 cells in the 44 samples of 2 WBCs/μL, and more than 100 cells in the 29 samples of 3∼5 WBCs/μL, respectively. In addition, extremely normal lymphocyte, monocyte and polymorphonuclear neutrophil were observed in the 143 samples of 0∼5 WBCs/μL. Macrophage and eosinophil were also rarely observed. The nucleated cell number for cells recovered on slide was 20 cells, which were regarded as 1 WBC/μL in body fluid cell count. However, in this study, we made alterations to report nucleated cell percentage as 0% without preparing the cytocentrifuged slide at 0 WBC/μL by using the cell yield in a comparison between the value of 0∼5 WBCs/μL and nucleated cell number for cells recovered on slide.
참고문헌 (27)
- Abbott BL, "Clinical significance of central nervous system involvement at diagnosis of pediatric acute myeloid leukemia: a single institution’s experience", Leukemia, 17, pp.2090~2096 (2003)
- Aulesa C, "Use of the Advia-120 hematology analyzer in the differential cytologic analysis of biological fluids (cerebrospinal, peritoneal, pleural, pericardial, synovial, and others)", Lab Hematol, 9, pp.214~224 (2003)
- Bames PW, "An evaluation of the utility of performing body fluid counts on the Coulter LH-750", Lab Hematol, 10, pp.127~131 (2004)
- Chapin-Robertson K, "Clinical and laboratory analysis of cytopsin-prepared Gram stains for recovery and diagnosis of from sterile body fluids", J Clin Microbiol, 30, pp.377~380 (1992)
- Chow G, "Lysis of erythrocytes and leukocytes in traumatic lumbar punctures", Arch Neurol, 41, pp.1084~1085 (1984)
- Clinical and Laboratory Standards Institute, "Body fluid analysis for cellular composition; approved guideline", Clinical and Laboratory Standards Institute, 6~41 (2006)
- College of American Pathologists, "Hematology and coagulation checklist", College of American Pathologists, 85~92 (2010)
- Collins L, "Body fluids. In Hematology", pp.592~ (2002), WB Saunders
- de Jonge R, "Automated analysis of pleural fluid total and differential leukocyte counts with the Sysmex XE-2100", Clin Chem Lab Med, 44, pp.1367~1371 (2006)
- Galagan KA, "Color atlas of body fluids", pp.12~13 (2009), College of American Pathologists
- Grover ML, "Effect of sample volume on cell recovery in cytocentrifugation", Acta Cytologica, 39, pp.387~390 (1995)
- Jones CD, "Wright-Giemsa cytology of body fluids: techniques for optimal cytocentrifuge slide preparation", Lab Med, 28, pp.713~716 (1997)
- Kjeldsberg CR, "Body fluids", pp.321~323 (1993), American Society for Clinical Pathology
- Kobayashi TK, "Evaluation of cytocentrifuge apparatus with special reference to the cellular recovery rate", Diagn Cytopathol, 8, pp.420~423 (1992)
- Linne JJ, "Basic techniques in clinical laboratory science", pp.413~ (1992), Mosby
- McPherson RA, "Henry’s clinical diagnosis and management by laboratory methods", pp.426~430 (2006), WB Saunders
- Muller TH, "Manual and automated methods for the determination of leukocyte counts at extreme low levels: comparative evaluation of the Nageotte chamber and the Abbott Cell Dyn 3500 analyzer", Transfus Sci, 18, pp.505~515 (1997)
- Nanos NE, "Evaluation of Sysmex UF-1000i for use in cerebrospinal fluid analysis", Clin Chim Acta, 392, pp.30~33 (2008)
- Rabinovitch A, "Body fluid microscopy in US laboratories. Data from two College of American Pathologists surveys, with practice recommendation", Arch Pathol Lab Med, 118, pp.13~17 (1994)
- Steele RW, "Leukocyte survival in cerebrospinal fluid", J Clin Microbiol, 23, pp.965~966 (1986)
- Strasinger SK, "Urinalysis and body fluids", pp.182~ (2008), FA Davis
- Timerley WR, "Cerebrospinal fluid examination and direct brain preparation. In Diagnostic cytopathology", pp.943~954 (2003), Churchil & Livingston
- "기준법령: 상대가치점수"[URL]
- 대한진단검사의학회, "진단검사의학", pp.432~ (2009), 이퍼블릭
- 유숙원, "새로운 혈구 수 측정법을 이용한 뇌척수액내 백혈구 수의 측정: 투명격자테이프를 이용한 방법", Annals of Laboratory Medicine, 27(6), pp.394~399 (2007), 대한진단검사의학회
- 이자영, "뇌척수액의 세포수 측정을 위한자동요침사분석기 UF-100의 유용성 평가", 임상병리와 정도관리, 29, pp.211~217 (2007)
- 이중달, "진단세포병리학", pp.315~319 (1999), 대학서림