Clinical Laboratory Aspect of Carbapenem-Resistant Enterobacteriaceae
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초록
The correct distinction of carbapenem-resistant Enterobacteriaceae (CRE) and ccarbapenemase producing Enterobacteriaceae (CPE) and the rapid detection of CPE are important for instituting the correct treatment and management of clinical infections. Screening protocols are mainly based on cultures of rectal swab specimens on selective media followed by phenotypic tests to confirm a carbapenem-hydrolyzing activity, the rapid carbapenem inactivation method, lateral flow immunoassay, the matrix-assisted laser desorption ionization–time-of-flight test and molecular methods. The CPE is accurate for detection, and is essential for the clinical treatment and prevention of infections. A variety of phenotypic methods and gene-based methods are available for the rapid detection of carbapenemases, and these are expected to be routinely used in clinical microbiology laboratories. Therefore, to control the spread of carbapenemase, many laboratories around the world will need to use reliable, fast, high efficiency, simple and low cost methods. Optimal effects in patient applications would require rapid testing of CRE to provide reproducible support for antimicrobial management interventions or the treatment by various types of clinicians. For the optimal test method, it is necessary to combine complementary test methods to discriminate between various resistant bacterial species and to discover the genetic diversity of various types of carbapenemase for arriving at the best infection control strategy.
Abstract
The correct distinction of carbapenem-resistant Enterobacteriaceae (CRE) and ccarbapenemase producing Enterobacteriaceae (CPE) and the rapid detection of CPE are important for instituting the correct treatment and management of clinical infections. Screening protocols are mainly based on cultures of rectal swab specimens on selective media followed by phenotypic tests to confirm a carbapenem-hydrolyzing activity, the rapid carbapenem inactivation method, lateral flow immunoassay, the matrix-assisted laser desorption ionization–time-of-flight test and molecular methods. The CPE is accurate for detection, and is essential for the clinical treatment and prevention of infections. A variety of phenotypic methods and gene-based methods are available for the rapid detection of carbapenemases, and these are expected to be routinely used in clinical microbiology laboratories. Therefore, to control the spread of carbapenemase, many laboratories around the world will need to use reliable, fast, high efficiency, simple and low cost methods. Optimal effects in patient applications would require rapid testing of CRE to provide reproducible support for antimicrobial management interventions or the treatment by various types of clinicians. For the optimal test method, it is necessary to combine complementary test methods to discriminate between various resistant bacterial species and to discover the genetic diversity of various types of carbapenemase for arriving at the best infection control strategy.
참고문헌 (56)
- Logan LK, "The epidemiology of carbapenem-resistant Enterobacteriaceae : the impact and evolution of a global menace", J Infect Dis, 215(Suppl 1), pp.28~36 (2017)[DOI]
- Evan J. Zasowski, "The β-Lactams Strike Back: Ceftazidime-Avibactam", Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy, 35(8), pp.755~770 (2015), Wiley[DOI]
- R. Köck, "Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review", Clinical Microbiology and Infection, 24(12), pp.1241~1250 (2018), Elsevier BV[DOI]
- Marion Vittecoq, "VIM-1 carbapenemase-producing Escherichia coli in gulls from southern France", Ecology and Evolution, 7(4), pp.1224~1232 (2017), Wiley[DOI]
- Gautier G, "Detection of different classes of carbapenemases : adaptation and assessment of a phenotypic method applied to Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii, and proposal of a new algorithm", J Microbiol Methods, 147, pp.26~35 (2018)[DOI]
- Hesna Yigit, "Novel Carbapenem-Hydrolyzing β-Lactamase, KPC-1, from a Carbapenem-Resistant Strain of Klebsiella pneumoniae", Antimicrobial Agents and Chemotherapy, 45(4), pp.1151~1161 (2001), American Society for Microbiology[DOI]
- Dongeun Yong, "Characterization of a New Metallo-β-Lactamase Gene, blaNDM-1, and a Novel Erythromycin Esterase Gene Carried on a Unique Genetic Structure in Klebsiella pneumoniae Sequence Type 14 from India", Antimicrobial Agents and Chemotherapy, 53(12), pp.5046~5054 (2009), American Society for Microbiology[DOI]
- Zhongju Chen, "First Report in China of Enterobacteriaceae Clinical Isolates Coharboring bla NDM-1 and bla IMP-4 Drug Resistance Genes", Microbial Drug Resistance, 21(2), pp.167~170 (2015), Mary Ann Liebert Inc[DOI]
- Biwei Yang, "Occurrence of Enterobacter hormaechei carrying bla NDM-1 and bla KPC-2 in China", Diagnostic Microbiology and Infectious Disease, 90(2), pp.139~142 (2018), Elsevier BV[DOI]
- Juan Wang, "First Report of Klebsiella oxytoca Strain Simultaneously Producing NDM-1, IMP-4, and KPC-2 Carbapenemases", Antimicrobial Agents and Chemotherapy, 61(9), pp.E0877-17~ (2017), American Society for Microbiology[DOI]
- Jorgensen SCJ, "Real-world experience with ceftazidime-avibactam for multidrug-resistant gram-negative bacterial infections", Open Forum Infect Dis, 6, pp.ofz522~ (2019)[DOI]
- Sofia Vourli, "Novel GES", IBC extended-spectrum β-lactamase variants with carbapenemase activity in clinical enterobacteria, 2004, pp.234(2), 209~213[DOI]
- Amélie Carrër, "Spread of OXA-48-Positive Carbapenem-Resistant Klebsiella pneumoniae Isolates in Istanbul, Turkey", Antimicrobial Agents and Chemotherapy, 52(8), pp.2950~2954 (2008), American Society for Microbiology[DOI]
- Assia Mairi, "OXA-48-like carbapenemases producing Enterobacteriaceae in different niches", European Journal of Clinical Microbiology & Infectious Diseases, 37(4), pp.587~604 (2018), Springer Science and Business Media LLC[DOI]
- Romney M. Humphries, "First Report of Ceftazidime-Avibactam Resistance in a KPC-3-Expressing Klebsiella pneumoniae Isolate", Antimicrobial Agents and Chemotherapy, 59(10), pp.6605~6607 (2015), American Society for Microbiology[DOI]
- Hong Zhong, "Accuracy and applicability of different phenotypic methods for carbapenemase detection in Enterobacteriaceae: A systematic review and meta-analysis", Journal of Global Antimicrobial Resistance, 21, pp.138~147 (2020), Elsevier BV[DOI]
- Carole Ayoub Moubareck, "Assessment of the performance of CHROMagar KPC and Xpert Carba-R assay for the detection of carbapenem-resistant bacteria in rectal swabs: First comparative study from Abu Dhabi, United Arab Emirates", Journal of Global Antimicrobial Resistance, 20, pp.147~152 (2020), Elsevier BV[DOI]
- Ozlem Genc, "Chromogenic culture media or rapid immunochromatographic test: Which is better for detecting Klebsiella pneumoniae that produce OXA-48 and can they be used in blood and urine specimens", Journal of Microbiological Methods, 148, pp.169~173 (2018), Elsevier BV[DOI]
- Vladimira Hinić, "Comparison of two rapid biochemical tests and four chromogenic selective media for detection of carbapenemase-producing Gram-negative bacteria", Journal of Microbiological Methods, 135, pp.66~68 (2017), Elsevier BV[DOI]
- Shawn Vasoo, "Comparison of the CHROMagar™ KPC, Remel Spectra™ CRE, and a direct ertapenem disk method for the detection of KPC-producing Enterobacteriaceae from perirectal swabs", Diagnostic Microbiology and Infectious Disease, 78(4), pp.356~359 (2014), Elsevier BV[DOI]
- P. Zarakolu, "Evaluation of a new chromogenic medium, chromID OXA-48, for recovery of carbapenemase-producing Enterobacteriaceae from patients at a university hospital in Turkey", European Journal of Clinical Microbiology & Infectious Diseases, 34(3), pp.519~525 (2015), Springer Science and Business Media LLC[DOI]
- Perry JD, "Prevalence of faecal carriage of Enterobacteriaceae with NDM-1 carbapenemase at military hospitals in Pakistan, and evaluation of two chromogenic media", J Antimicrob Chemother, 66, pp.2288~2294 (2011)[DOI]
- Morgan A. Pence, "Comparison of Chromogenic Media for Recovery of Carbapenemase-Producing Enterobacteriaceae (CPE) and Evaluation of CPE Prevalence at a Tertiary Care Academic Medical Center", Journal of Clinical Microbiology, 53(2), pp.663~666 (2015), American Society for Microbiology[DOI]
- Elizabeth B. Hirsch, "An evaluation of multiple phenotypic screening methods for Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae", Journal of Infection and Chemotherapy, 20(3), pp.224~227 (2014), Elsevier BV[DOI]
- Patricia J. Simner, "Evaluation of Five Chromogenic Agar Media and the Rosco Rapid Carb Screen Kit for Detection and Confirmation of Carbapenemase Production in Gram-Negative Bacilli", Journal of Clinical Microbiology, 53(1), pp.105~112 (2014), American Society for Microbiology[DOI]
- Ergina Malli, "Evaluation of rapid polymyxin NP test to detect colistin-resistant Klebsiella pneumoniae isolated in a tertiary Greek hospital", Journal of Microbiological Methods, 153, pp.35~39 (2018), Elsevier BV[DOI]
- Vincent J. LaBombardi, "Evaluation of Remel Spectra CRE Agar for Detection of Carbapenem-Resistant Bacteria from Rectal Swabs Obtained from Residents of a Long-Term-Care Facility", Journal of Clinical Microbiology, 53(9), pp.2823~2826 (2015), American Society for Microbiology[DOI]
- Tomokazu Kuchibiro, "Evaluation of the modified carbapenem inactivation method for the detection of carbapenemase-producing Enterobacteriaceae", Journal of Infection and Chemotherapy, 24(4), pp.262~266 (2018), Elsevier BV[DOI]
- Vincent van Almsick, "Rapid detection of carbapenemase-producing Acinetobacter baumannii and carbapenem-resistant Enterobacteriaceae using a bioluminescence-based phenotypic method", Journal of Microbiological Methods, 147, pp.20~25 (2018), Elsevier BV[DOI]
- Pierre Bogaerts, "Evaluation of the BYG Carba Test, a New Electrochemical Assay for Rapid Laboratory Detection of Carbapenemase-Producing Enterobacteriaceae", Journal of Clinical Microbiology, 54(2), pp.349~358 (2016), American Society for Microbiology[DOI]
- Hossein Salimnia, "Evaluation of the FilmArray Blood Culture Identification Panel: Results of a Multicenter Controlled Trial", Journal of Clinical Microbiology, 54(3), pp.687~698 (2016), American Society for Microbiology[DOI]
- M. Tato, "Multisite Evaluation of Cepheid Xpert Carba-R Assay for Detection of Carbapenemase-Producing Organisms in Rectal Swabs", Journal of Clinical Microbiology, 54(7), pp.1814~1819 (2016), American Society for Microbiology[DOI]
- Vanessa Gaia Rocco, "Rapid Identification of Carbapenemase-producing Klebsiella pneumoniae strains by Matrix-Assisted Laser Desorption", Ionization-Time of Flight using Vitek® Mass Spectrometry System, 2019, pp.51(3), 209~213[DOI]
- Jiajia Yu, "Rapid detection of carbapenemase activity of Enterobacteriaceae isolated from positive blood cultures by MALDI-TOF MS", Annals of Clinical Microbiology and Antimicrobials, 17(1), pp.22~ (2018), Springer Science and Business Media LLC[DOI]
- Ellionor Rapp, "Detection of carbapenemases with a newly developed commercial assay using Matrix Assisted Laser Desorption Ionization-Time of Flight", Journal of Microbiological Methods, 146, pp.37~39 (2018), Elsevier BV[DOI]
- Dortet L, "Evaluation of the RAPIDECⓇ CARBA NP, the Rapid CARB ScreenⓇand the Carba NP test for biochemical detection of carbapenemase-producing Enterobacteriaceae", J Antimicrob Chemother, 70, pp.3014~3022 (2015)[DOI]
- Nils Kunze, "Point-of-care multiplex PCR promises short turnaround times for microbial testing in hospital-acquired pneumonia – an observational pilot study in critical ill patients", Annals of Clinical Microbiology and Antimicrobials, 14(1), pp.33~ (2015), Springer Science and Business Media LLC[DOI]
- Nathan A. Ledeboer, "Identification of Gram-Negative Bacteria and Genetic Resistance Determinants from Positive Blood Culture Broths by Use of the Verigene Gram-Negative Blood Culture Multiplex Microarray-Based Molecular Assay", Journal of Clinical Microbiology, 53(8), pp.2460~2472 (2015), American Society for Microbiology[DOI]
- Yi Wang, "Multiplex, Rapid, and Sensitive Isothermal Detection of Nucleic-Acid Sequence by Endonuclease Restriction-Mediated Real-Time Multiple Cross Displacement Amplification", Frontiers in Microbiology, 7, pp.753~ (2016), Frontiers Media SA[DOI]
- Shoukui Hu, "Identification of Acinetobacter baumannii and its carbapenem-resistant gene blaOXA-23-like by multiple cross displacement amplification combined with lateral flow biosensor", Scientific Reports, 9(1), pp.17888~ (2019), Springer Science and Business Media LLC[DOI]
- Amy J. Mathers, "Klebsiella pneumoniae Carbapenemase (KPC)-Producing K. pneumoniae at a Single Institution: Insights into Endemicity from Whole-Genome Sequencing", Antimicrobial Agents and Chemotherapy, 59(3), pp.1656~1663 (2015), American Society for Microbiology[DOI]
- Cancan Cheng, "Rapid Detection of bla NDM , bla KPC , bla IMP , and bla VIM Carbapenemase Genes in Bacteria by Loop-Mediated Isothermal Amplification", Microbial Drug Resistance, 20(6), pp.533~538 (2014), Mary Ann Liebert Inc[DOI]
- Frickmann H, "Emerging rapid resistance testing methods for clinical microbiology laboratories and their potential impact on patient management", Biomed Res Int, 2014, pp.375681~ (2014)[DOI]
- Haofu Niu, "Rapid Nanopore Assay for Carbapenem-Resistant Klebsiella pneumoniae", Frontiers in Microbiology, 10, pp.1672~ (2019), Frontiers Media SA[DOI]
- Kim JS, "Evaluation of Verigene blood culture test systems for rapid identification of positive blood cultures", Biomed Res Int, 2016, pp.1081536~ (2016)[DOI]
- Rosemary Swayne, "Utility of a novel multiplex TaqMan PCR assay for metallo-β-lactamase genes plus other TaqMan assays in detecting genes encoding serine carbapenemases and clinically significant extended-spectrum β-lactamases", International Journal of Antimicrobial Agents, 42(4), pp.352~356 (2013), Elsevier BV[DOI]
- Delphine Girlich, "Evaluation of the BD MAX Check-Points CPO Assay for the Detection of Carbapenemase Producers Directly from Rectal Swabs", The Journal of Molecular Diagnostics, 22(2), pp.294~300 (2020), Elsevier BV[DOI]
- Imane Saad Albichr, "Evaluation of the automated BD Phoenix CPO Detect test for detection and classification of carbapenemases in Gram negatives", Diagnostic Microbiology and Infectious Disease, 96(2), pp.114911~ (2020), Elsevier BV[DOI]
- Jung-Hyun Byun, "An agar plate-based modified carbapenem inactivation method (p-mCIM) for detection of carbapenemase-producing Enterobacteriaceae", Journal of Microbiological Methods, 168, pp.105781~ (2020), Elsevier BV[DOI]
- Xiaopeng Jing, "The Rapid Carbapenemase Detection Method (rCDM) for Rapid and Accurate Detection of Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa", Frontiers in Cellular and Infection Microbiology, 9, pp.371~ (2019), Frontiers Media SA[DOI]
- Masanori Watahiki, "Single-Tube Multiplex Polymerase Chain Reaction for the Detection of Genes Encoding Enterobacteriaceae Carbapenemase", Japanese Journal of Infectious Diseases, 73(2), pp.166~172 (2020), Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis[DOI]
- Gina K. Thomson, "CPO Complete, a novel test for fast, accurate phenotypic detection and classification of carbapenemases", PLOS ONE, 14(12), pp.e0220586~ (2019), Public Library of Science (PLoS)[DOI]
- Jung-Hyun Byun, "Evaluation of Xpert Carba-R Assay v.2 to Detect Carbapenemase Genes in Two Hospitals in Korea", Annals of Laboratory Medicine, 40(3), pp.209~215 (2020), Annals of Laboratory Medicine[DOI]
- Francesca Del Bianco, "Comparison of Four Commercial Screening Assays for the Detection of blaKPC, blaNDM, blaIMP, blaVIM, and blaOXA48 in Rectal Secretion Collected by Swabs", Microorganisms, 7(12), pp.704~ (2019), MDPI AG[DOI]
- Elizabeth Pinet, "A simple phenotypic test for detecting the contribution of outer membrane permeability to carbapenem resistance", Journal of Medical Microbiology, 69(1), pp.63~71 (2020), Microbiology Society[DOI]
- Delphine Girlich, "Evaluation of the Revogene Carba C Assay for Detection and Differentiation of Carbapenemase-Producing Gram-Negative Bacteria", Journal of Clinical Microbiology, 58(4) (2020), American Society for Microbiology[DOI]
