조직 표본의 병원체 증명에 었어서 Epon-toluidine blue 염색과 전자현미경적 검색 -P. carinii의 미세구조 관찰 -

Abstract

Pneumocystis carinii is well known as a opportunistic pathogens. Pneumocystis pneumonia is thought to be occured in immunodeficient patients by reactivation of a latent infection and immune function is responsible for maintaining P. carinii in latent state. Although the structure and characters of various infectious pathogens is progressively knowning due to the development of electron microscopical and molecular bological techiques, P. carinii phylogeny has not been determined with certainty ye t. P. carinii can be diagnosed after direct smear and special staining of sputum or BAL specimens. Since an observer’ s subjective state or equivocal shape can make wrong diagnosis, electron microscopical observation is performed to detect P. carinii more accurately. Rats were immunosuppressed by weekly subcutaneous injection of 20 mg/kg methylprednisolone. on the 6th week of immunosuppression , the lungs were removed and fixed for electron microscopical observation. Both trophic and cystic forms of P. carinii could be detected. Trophic forms have been obseπed 2-5 um in size with much variation and multishape of surface, have one nucleus for each trophic form. Cysts have various number of intracystic bodies and are surrounded by thick well. The empty cyst wall remained banana shape. P. cariniÍ was easily detected in immunosuppressed rat lung tissues using electronmicroscope. So, ultrastructural observation might help in correct diagnosis of pathogenic organism located in biopsied tissues.