자궁경부의 편평상피내병소와 편평상피세포암종에서의 비만세포에 관한 연구

Abstract

Mast cells have recently been found to be well correlated with the inhibition of invasiveness and metastasis in various cancer. This work aimed to investigate the number of mast cells and ratio of mast cell granulation in squamous intraepithelial lesions and squamous cell carcinoma of the cervix. Cervical biopsy specimens were divided into low grade squarnous intraepithelial lesion(n=20), high grade squamous intraepithelial lesion(n=75) and squamous cell carcinoma(n=40) by The Bethesda System. The sections were cut from routinely formalin- fixed and paraffin-embedded tissues and stained with 0.5% toluidine blue(pH0.5)-nuclear fast red. The mast cells were quantificated the numbers per square millimeter using a computerized image analysis system. Mean number of mast cells was significantly higher in high grade squamous intraepithelial lesion than in low grade squamous intraepithelial lesion and significantly lower in squamous cell carcinoma than in high grade squamous intraepithelial lesion(p<0.01). The percent of mast cell ganulation also was significantly higher in high grade squamous intraepithelial lesion than in lowgrade squamus intraeptithelial lesion and significantly lower in squamous cell carcinoma than in high grade intraepithelial lesion (p<0.01). These results suggested that mast cell number and percent of mast cell granulation were to be correlated to the development from precancerous lesion to cervical cancerous lesion and may be useful as markers of cervical squamous cell carcinoma.