Reference Values for Peripheral Blood Lymphocyte Subsets in a Healthy Korean Population
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초록
Flow cytometric immunophenotyping of peripheral bloodlymphocyte subsets is a powerful tool for evaluating cellularimmunity and monitoring immune-mediated diseases. Thenumbers and proportions of blood lymphocyte subsets are influencedby factors such as gender, age, ethnicity, andlifestyle. This study aimed to establish reference ranges forperipheral blood lymphocyte subsets in a healthy Koreanpopulation. Blood samples from 294 healthy adults werecollected. Lymphocyte subsets were analyzed using a single-platform method with a flow cytometer; white blood cellsand lymphocytes were analyzed using an automated hematologyanalyzer. The mean value of the white blood cell countwas 5,665 cells/μl, and the mean values of the subtypecounts (percentages) were as follows: lymphocytes, 1,928cells/μl (35.08%); CD3＋ cells, 1,305 cells/μl (67.53%);CD3＋CD4＋ cells, 787 cells/μl (40.55%); CD3＋CD8＋ cells,479 cells/μl (25.23%); CD3−CD19＋ cells, 203 cells/μl(10.43%); and CD3−CD56＋ cells, 300 cells/μl (15.63%). Additionally, the CD4＋/CD8＋ ratio was 1.81. In this study,gender and age significantly influenced blood lymphocytesubsets. Our results demonstrate that, as with other populations,a healthy Korean population has its own, region-specific, lymphocyte subset reference ranges.
Abstract
Flow cytometric immunophenotyping of peripheral bloodlymphocyte subsets is a powerful tool for evaluating cellularimmunity and monitoring immune-mediated diseases. Thenumbers and proportions of blood lymphocyte subsets are influencedby factors such as gender, age, ethnicity, andlifestyle. This study aimed to establish reference ranges forperipheral blood lymphocyte subsets in a healthy Koreanpopulation. Blood samples from 294 healthy adults werecollected. Lymphocyte subsets were analyzed using a single-platform method with a flow cytometer; white blood cellsand lymphocytes were analyzed using an automated hematologyanalyzer. The mean value of the white blood cell countwas 5,665 cells/μl, and the mean values of the subtypecounts (percentages) were as follows: lymphocytes, 1,928cells/μl (35.08%); CD3＋ cells, 1,305 cells/μl (67.53%);CD3＋CD4＋ cells, 787 cells/μl (40.55%); CD3＋CD8＋ cells,479 cells/μl (25.23%); CD3−CD19＋ cells, 203 cells/μl(10.43%); and CD3−CD56＋ cells, 300 cells/μl (15.63%). Additionally, the CD4＋/CD8＋ ratio was 1.81. In this study,gender and age significantly influenced blood lymphocytesubsets. Our results demonstrate that, as with other populations,a healthy Korean population has its own, region-specific, lymphocyte subset reference ranges.
참고문헌 (25)
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